Difference between revisions of "Team:UC San Diego/Notebook"

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<a href="/Team:UC_San_Diego/Practices#library" class="cd-read-more">Read more.</a>
 
<a href="/Team:UC_San_Diego/Practices#library" class="cd-read-more">Read more.</a>
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<span class="cd-date">Week 14</span>
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<span class="cd-date">WEEK 14</span>
 
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<span class="cd-date">WEEK 14</span>
 
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<p> +Mapped out errors in the parts that we assembled
 +
                                <br>+ Q5 Mutagenesis to fix recurring errors in C sequence
 +
</p>
 
<span class="cd-date">WEEK 13</span>
 
<span class="cd-date">WEEK 13</span>
 
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<span class="cd-date">WEEK 12</span>
 
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<p></p>
 
<span class="cd-date">WEEK 9</span>
 
<span class="cd-date">WEEK 9</span>
 
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<h2>Wet Lab</h2>
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<p>+ SGI finally sent us our DNA fragments.
 +
                                <br>+ Attempted to PCR and transform our fragments.</p>
 
<span class="cd-date">WEEK 8</span>
 
<span class="cd-date">WEEK 8</span>
 
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<h2>Wet Lab</h2>
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<p>+ Assembled Interlab Devices. Resuspended and measured them successfully.
 +
                                <br>+ Prepared YPD plates.</p>
 
<span class="cd-date">WEEK 7</span>
 
<span class="cd-date">WEEK 7</span>
 
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<h2>Wet Lab</h2>
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<p>+ Continued interlab study with miniprep, restriction digests, ligation, gel electrophoresis and gel purification.</p>
 
<span class="cd-date">WEEK 6</span>
 
<span class="cd-date">WEEK 6</span>
 
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<h2>Wet Lab</h2>
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<p>Started interlab study.</p>
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<p>+ Started interlab study with transformation.</p>
 
<span class="cd-date">WEEK 5</span>
 
<span class="cd-date">WEEK 5</span>
 
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<h2>Wet Lab</h2>
 
<h2>Wet Lab</h2>
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<p>+Sent our genes to SGI and ordered lab supplies. Almost ready to go!</p>
+
<p>+ Sent our genes to SGI and ordered lab supplies. Almost ready to go!</p>
 
<span class="cd-date">WEEK 4</span>
 
<span class="cd-date">WEEK 4</span>
 
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<h2>Wet Lab</h2>
 
<h2>Wet Lab</h2>
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<p>Added frp gene sequence from Vibrio Harveyi to our plasmid to stabilize the production of luciferase.
+
<p>+ Added frp gene sequence from Vibrio Harveyi to our plasmid to stabilize the production of luciferase.
−
<br>+Optimized our plasmid sequences.
+
<br>+ Optimized our plasmid sequences.
 
</p>
 
</p>
 
<span class="cd-date">WEEK 3</span>
 
<span class="cd-date">WEEK 3</span>
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<div class="cd-timeline-content">
 
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<h2>Wet Lab</h2>
 
<h2>Wet Lab</h2>
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<p>+Created a preliminary design for the plasmids using ApE.
+
<p>+ Created a preliminary design for the plasmids using ApE.
−
<br>+Improved them over the week by adding tags, removed illegal restriction sites, and changing repetitive sequences.
+
<br>+ Improved them over the week by adding tags, removed illegal restriction sites, and changing repetitive sequences.
  
 
</p>
 
</p>

Revision as of 04:35, 18 September 2015

TIMELINE

September 24-28

Giant Jamboree!

Giant Jamboree!

September 16

Library presentation at La Jolla Riford Library.

Read more. WEEK 14

Computational

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WEEK 14

Wet Lab

+

WEEK 14

Title of section 2

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Read more about what we did WEEK 13

Section 13

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WEEK 13

Wet Lab

+Mapped out errors in the parts that we assembled
+ Q5 Mutagenesis to fix recurring errors in C sequence

WEEK 13

September 4

High school meet up.

Read more. WEEK 12

Section 13

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WEEK 12

Wet Lab

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WEEK 12

Section 13

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WEEK 11

Section 13

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WEEK 11

week ##

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WEEK 11

Section 13

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WEEK 10

Section 13

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WEEK 10

week 10

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WEEK 10

week 9

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WEEK 9/span>

week 9

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WEEK 9

Wet Lab

WEEK 9

August 6

Southern California iGEM Meetup

Read more. Week 8

Section 13

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WEEK 8

Wet Lab

+ SGI finally sent us our DNA fragments.
+ Attempted to PCR and transform our fragments.

WEEK 8

Section 13

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WEEK 7

Section 13

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WEEK 7

Wet Lab

+ Assembled Interlab Devices. Resuspended and measured them successfully.
+ Prepared YPD plates.

WEEK 7

Section 13

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WEEK 6

Section 13

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WEEK 6

Wet Lab

+ Continued interlab study with miniprep, restriction digests, ligation, gel electrophoresis and gel purification.

WEEK 6

Section 13

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WEEK 5

Section 13

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WEEK 5

Wet Lab

+ Started interlab study with transformation.

WEEK 5

Section 13

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WEEK 4

Section 13

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WEEK 4

Wet Lab

+ Sent our genes to SGI and ordered lab supplies. Almost ready to go!

WEEK 4

Section 13

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WEEK 3

Section 13

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WEEK 3

Wet Lab

+ Added frp gene sequence from Vibrio Harveyi to our plasmid to stabilize the production of luciferase.
+ Optimized our plasmid sequences.

WEEK 3

Website

More website ideas.

WEEK 2

Computational

Read papers on drive
Read about metabolic control analysis
Compiled notes on bioluminescent system
Literature research:understanding bioluminescent mechanism
> aldehyde synthesis
> FMNH2 production
> light production
Familiarized with COBRA module

Primer to Genome-Scale Modeled and COBRA Tutorial (Jahir)

Continued literature search for key mechanisms regarding our auto-induced bioluminescent reaction in a yeast model
>auto-induced therefore no coupling between cells (removed LuxI and LuxR genes)

WEEK 2

Wet Lab

+ Created a preliminary design for the plasmids using ApE.
+ Improved them over the week by adding tags, removed illegal restriction sites, and changing repetitive sequences.

WEEK 2

Website

Discussed and designed website prototypes.

WEEK 1

Computational

Developed Primer to MATLAB Programming
> lecture material from MIT course and UCSD course
> reading material from UCSD course
> study and practice material MIT course and UCSD course

WEEK 1

Wet Lab

+ Found genes coding for fatty acid reductase complex that have been validated in an in vitro synthesis paper.
+ Planned how to assemble our plasmids and determined nucleotide sequences for lux A-E of Photobacterium Phosphoreum.
+ Compared the amino acid sequences to those of other organisms on BLAST to check for significant discrepancies in our sequence.

WEEK 1