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The collection of parts described herein forms a modular platform to determine which of the enzymes of the fatty acid reductase complex is rate-limiting. Each permutation of the complex’s genes is compatible for coexpression with the LuxAB construct. This methodology of stoichiometric expression can also be used to determine rate-limiting steps in other metabolic systems.
LuxABfrp - Coding - Engineered LuxAB with Vibrio Harveyi frp; 2A linker present between each protein for stoichiometric expression.
LuxCDEC - Coding - Photobacterium Phosphoreum fatty acid reductase complex with overexpressed reductase; 2A linker present between each protein for stoichiometric expression.
LuxCDED - Coding - Photobacterium Phosphoreum fatty acid reductase complex with overexpressed transferase; 2A linker present between each protein for stoichiometric expression.
LuxCDEE - Coding - Photobacterium Phosphoreum fatty acid reductase complex with overexpressed synthetase; 2A linker present between each protein for stoichiometric expression.
LuxDEC - Coding - Photobacterium Phosphoreum fatty acid reductase complex with even stoichiometry for LuxCDE. 2A linker present between each protein for stoichiometric expression.