Difference between revisions of "Team:Columbia NYC/Safety"

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<h2>Safety in iGEM</h2>
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<p>Please visit <a href="https://2015.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h4>Safe Project Design</h4>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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</ul>
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<h4>Safe Lab Work</h4>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h4>Safe Shipment</h4>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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<a href= "https://2015.igem.org/Team:Columbia_NYC"> <img src="https://static.igem.org/mediawiki/2015/c/ca/Columbia_NYC_Logo.jpg" alt="Columbia iGEM Logo" style="width:100%"> </a>
  
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<a href = "#"> <li> THE PROJECT
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<ul class = "subTab">
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<a href = "https://2015.igem.org/Team:Columbia_NYC/Description"> <li> DESCRIPTION </li></a>
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<a href = "https://2015.igem.org/Team:Columbia_NYC/Design"> <li> DESIGN </li></a>
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<a href = "https://2015.igem.org/Team:Columbia_NYC/Experiments"> <li> EXPERIMENTS </li></a>
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<a href = "https://2015.igem.org/Team:Columbia_NYC/Results"> <li> RESULTS </li></a>
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</ul>
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</li></a>
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<a href = "https://2015.igem.org/Team:Columbia_NYC/Team"> <li> TEAM MEMBERS </li></a>
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<a href = "https://2015.igem.org/Team:Columbia_NYC/Parts"> <li> TEAM PARTS </li></a>
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<a href="https://2015.igem.org/Team:Columbia_NYC/Notebook"><li> THE NOTEBOOK </li></a>
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<a href = "https://2015.igem.org/Team:Columbia_NYC/Attributions"> <li> ATTRIBUTIONS </li></a>
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<a href = "https://2015.igem.org/Team:Columbia_NYC/Collaborations"><li> COLLABORATIONS </li></a>
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<a href = "https://2015.igem.org/Team:Columbia_NYC/Practices"><li> HUMAN PRACTICE </li></a>
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<a href="https://2015.igem.org/Team:Columbia_NYC/Safety"><li> LAB SAFETY </li></a>
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<a href = "https://2015.igem.org/Team:Columbia_NYC/Sponsors"> <li> OUR SPONSORS </a>
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<h1> LAB SAFETY </h1>
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<p> Working in a laboratory can have its hazards. As a result, we ensured that we took steps to keep ourselves safe and out of harm's way by taking the following steps: </p>
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<h2> <b> Safe Project Design </b> </h2>
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<p> In order to reduce the risks that our team members were exposed to while working on the project, certain decisions about the project design were made. </p>
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<ul>
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<li> <b> Choice of Chassis</b>: The chassis organisms we worked with are all BSL-1 organisms and fall within Risk Group 1 by NIH guidelines. The specific chassis used are: <i> Escherichia coli </i> MegaX, Nissle, and K12 and <i> Lactobacillus reuteri </i> and <i>casei </i>. As such, team members were not exposed to potentially harmful bacteria. </li>
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<li> <b> Choice of Parts</b>: The genetic parts we were working with were are, by the nature of our project, safe for humans. The signal peptides we are working with can be found naturally in <i> E. coli </i>and <i> Lactobacillus </i> and are not shown to impact human health and the gut peptides GLP-1, PYY, and Ghrelin are already naturally found in humans. In addition, the lysis mechanism involves the use of holins and endolysins that are not harmful to humans. </li>
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<li> <b> "Kill Switch" Device</b>: In addition to the secretion of gut peptides, the design of our device includes a "kill switch" device that will cause cells to undergo a delayed cell-death after the secretion of gut peptides. This system is regulated by AHL-based quorum sensing. For more information, please visit our <a href = "https://2015.igem.org/Team:Columbia_NYC/Design"> project design page</a>. </li> 
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</ul>
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<h2> <b> Safe Lab Work </b> </h2>
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<p> In order to ensure the safety of all of our team members, proper training had to be provided and safe lab procedures to be carried out on a daily basis. The following are steps taken to meet this goal: </p>
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<ul>
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<li> <b> Safety Training </b> : All team members completed the training and received certificates for "Lab Safety, Chemical Hygiene, and Waste Management", "Recombinant DNA Training", and "Biological/Bloodborne Pathogen Training" that is given by Columbia University. As such, team members are trained to work in a laboratory setting and handle potential emergencies before starting experiments. </li>
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<li> <b> Proper Lab Attire (PPE)</b>: In order to decrease potential exposures to biological materials and chemical reagents, team members are required to wear closed-toe shoes, long pants, and gloves while at the bench. In addition, goggles were used when necessary (i.e during exposures to higher frequency light). </li>
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<li> <b> Using Safe Substitutes</b>: To further decrease the number of potential risks in the laboratory, we used lab procedures that had the least inherent risks. Most notably, this involved the use of SYBR Gold E-Gel over agarose gels that need to be stained by the carcinogenic ethidium bromide. </li>
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</div>

Latest revision as of 00:52, 19 September 2015

LAB SAFETY

Working in a laboratory can have its hazards. As a result, we ensured that we took steps to keep ourselves safe and out of harm's way by taking the following steps:

Safe Project Design

In order to reduce the risks that our team members were exposed to while working on the project, certain decisions about the project design were made.

  • Choice of Chassis: The chassis organisms we worked with are all BSL-1 organisms and fall within Risk Group 1 by NIH guidelines. The specific chassis used are: Escherichia coli MegaX, Nissle, and K12 and Lactobacillus reuteri and casei . As such, team members were not exposed to potentially harmful bacteria.
  • Choice of Parts: The genetic parts we were working with were are, by the nature of our project, safe for humans. The signal peptides we are working with can be found naturally in E. coli and Lactobacillus and are not shown to impact human health and the gut peptides GLP-1, PYY, and Ghrelin are already naturally found in humans. In addition, the lysis mechanism involves the use of holins and endolysins that are not harmful to humans.
  • "Kill Switch" Device: In addition to the secretion of gut peptides, the design of our device includes a "kill switch" device that will cause cells to undergo a delayed cell-death after the secretion of gut peptides. This system is regulated by AHL-based quorum sensing. For more information, please visit our project design page.

Safe Lab Work

In order to ensure the safety of all of our team members, proper training had to be provided and safe lab procedures to be carried out on a daily basis. The following are steps taken to meet this goal:

  • Safety Training : All team members completed the training and received certificates for "Lab Safety, Chemical Hygiene, and Waste Management", "Recombinant DNA Training", and "Biological/Bloodborne Pathogen Training" that is given by Columbia University. As such, team members are trained to work in a laboratory setting and handle potential emergencies before starting experiments.
  • Proper Lab Attire (PPE): In order to decrease potential exposures to biological materials and chemical reagents, team members are required to wear closed-toe shoes, long pants, and gloves while at the bench. In addition, goggles were used when necessary (i.e during exposures to higher frequency light).
  • Using Safe Substitutes: To further decrease the number of potential risks in the laboratory, we used lab procedures that had the least inherent risks. Most notably, this involved the use of SYBR Gold E-Gel over agarose gels that need to be stained by the carcinogenic ethidium bromide.