Difference between revisions of "Team:OUC-China"

 
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  <div id="content">
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<!DOCTYPE html>
<a name="top" id="top"></a>
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<html lang="zh-CN">
<h1 class="firstHeading">Team:HKUST-Rice</h1>
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<div id="bodyContent">
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<p>
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<head>
<style type="text/css">
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/* WIKI SETTINGS*/
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.firstHeading {
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    <title>Team:OUC-China</title>
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/*left align Black Menu Bar */
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            .work-content .col-md-4{
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<style type="text/css">
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/* PAGE LAYOUT */
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text-align:center;
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+
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                        height: 75%;
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/*overflow:hidden;*/
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margin: 0px;
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text-align:center;
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} */
+
 
+
 
+
 
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/*VISUAL STYLE (TABLES, FONTS, LINKS, IMAGES, UL) */
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/*Change the styling of text for everything inside main container*/
+
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+
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font-weight: bold;
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  /* Styling links on hover- links are different inside the menu */
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+
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+
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+
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+
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+
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+
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+
    }
+
 
+
 
+
 
+
/*MENU STYLING */
+
 
+
#menuContainer {
+
text-align: center;
+
                                        padding-top: 10px;
+
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+
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+
 
+
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+
color: #FFF;
+
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+
+
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+
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+
margin-left: 14%;
+
padding: 0;
+
text-align: center;
+
                                        width: 70%
+
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+
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+
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text-align: center;
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+
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+
background: #000;
+
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+
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+
 
+
 
+
/* Level 1 Drop Down Menu */
+
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+
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+
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+
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+
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+
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+
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+
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+
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+
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+
+
+
/* Level 2 */
+
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+
text-align: left;
+
width: auto; /* change auto value with 200px if you want a bigger menu */
+
display: none;
+
background-color: #1b1c3a;
+
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+
top: 100%;
+
left: 0;
+
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+
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+
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+
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+
+
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+
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+
+
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+
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+
text-align: left;
+
display: none;
+
background: #1b1c3a;
+
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+
top: 0;
+
z-index: 9999999;
+
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+
#menuContainer > ul > li > ul > li:hover > ul {
+
display: block;
+
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+
#menuContainer > ul ul ul li {}
+
#menuContainer > ul ul ul li a {}
+
#menuContainer > ul ul ul li a:hover {}
+
#menuContainer.navItem {
+
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+
}
+
                        #Directory { 
+
width: 100%;
+
overflow:hidden;
+
float:left;
+
margin-left:20px;
+
                                margin-right: 20px;
+
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+
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+
border-bottom: 0px solid #565656;
+
border-right: 0px solid #565656;
+
border-left: 0px solid #565656;
+
border-top: 0px solid #565656;
+
font-family: "Trebuchet MS", Helvetica, sans-serif;
+
}               
+
                          /*Style the Directory*/     
+
                          #Directory table {
+
                                Background-color: #000000;
+
                                color: #FFFFFF;
+
                                margin-left: 0px;
+
                                padding: 20px;
+
                                line-height: 10px;
+
                                height: 100px;
+
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+
                                /*float:left;*/
+
                          }
+
               
+
 
+
                /*Style the Directory button*/     
+
                        #Directory li a{
+
                                color: #000000;
+
                                padding: 0px;
+
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+
 
+
img.button {
+
    opacity: 1.0;
+
    filter: alpha(opacity=100); /* For IE8 and earlier */
+
 
}
 
}
 
+
/* disable IGEM's style */
img.button:hover {
+
/*#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5, #bodyContent h6 {
    opacity: 0.7;
+
border-bottom: none;
    filter: alpha(opacity=80); /* For IE8 and earlier */
+
padding-top: 0;
 +
padding-bottom: 0;
 +
margin-bottom: 0;
 +
}
 +
#bodyContent p {
 +
font-size: 100%;
 +
line-height: 1em;
 +
}
 +
#bodyContent li {
 +
margin-bottom: 0;
 
}
 
}
 +
#bodyContent a {
 +
font-size: 100%;
 +
}*/
  
 +
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 +
</head>
  
/*CLASSES */
+
<body>
 
+
    <header class="header">
/*Clear class for all the pages, adds spacing too*/
+
        <nav class="navbar navbar-default">
.clear{
+
            <div class="container">
clear:both;
+
                <span class="menu"> Menu</span>
height: 10px;
+
                <div class="banner-top">
}
+
                    <ul class="nav banner-nav">
 
+
                        <li><a href="https://2015.igem.org/Team:OUC-China">HOME</a></li>
 
+
                        <li class="dropdown1"><a class="down-scroll" href="#">PROJECT</a>
/* highlight box for special messages */
+
                            <ul class="dropdown2">
    .highlightBox {
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Overview">Overview</a></li>
        width:500px;
+
                                 <li><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver">Magnetic Receiver</a></li>
                                 margin:auto;
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator">Thermosensitive Regulator</a></li>
        background-color: #E8E8E9;
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Captor">Captor</a></li>
margin-bottom: 15px;
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Results">Results</a></li>
margin-top: 15px;
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Parts">Parts</a></li>
padding: 15px;
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Project/Achievements">Achievements</a></li>
padding-top: 10px;
+
                            </ul>
    }
+
                        </li>
 
+
                        <li class="dropdown1"><a href="https://2015.igem.org/Team:OUC-China/Interlab">INTERLAB</a>
 
+
                        </li>
</style>
+
                        <li class="dropdown1"><a class="down-scroll" href="#">MODELING</a>
<!-- End of CSS -->
+
                            <ul class="dropdown2">
 
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling">Overview</a></li>
 
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Magnetic_Receiver">Magnetic Receiver</a></li>
<!-- Start of the template html elements. -->
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Heating">Heating</a></li>
<div id ="mainContainer"><!--The closing tag for mainContainer should be placed at the bottom of each content page.-->
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Modeling/Thermosensitive_Regulator">Thermosensitive Regulator</a></li>
 
+
                            </ul>
 
+
                        </li>
                <!-- <div id="titleContainer">
+
                        <li class="dropdown1"><a class="down-scroll" href="#">HUMAN PRACTISE</a>
<br>
+
                            <ul class="dropdown2">
                <image src="https://static.igem.org/mediawiki/2015/d/d4/Logo.jpg" style="width: 100px; height: 100px; float: left;">
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Practices">Overview</a></li>
 
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Safety">Safety</a></li>
        <p style="font-size:40px;color:#FFFFFF;line-height:1em;"> Potassium, Nitrate and Phosphate Biosensor </font><br>
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Education">Education</a></li>
                <font size= "5"> HKUST-Rice iGEM Team </font></p>
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Transportation">Transportation</a></li>
             
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Communication">Communication</a></li>
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Human_Practice/Guide_Of_Logo_Design">Guide of Logo Design</a></li>
        </div> -->
+
                            </ul>
 
+
                        </li>
                <!-- <div id="bannerContainer">
+
                        <li class="dropdown1"><a class="down-scroll" href="#">NOTE BOOK</a>
<p>You can make the image 980px  by  200px</p>
+
                            <ul class="dropdown2">
<p> Remember to call the file: "<i>Team_HKUST-Rice_banner.jpg</i>" </p>
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Lab_Journal">Lab Journal</a></li>
                 </div> -->
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Note_Book/Protocol">Protocol</a></li>
 
+
                            </ul>
        <!-- Start of menu -->
+
                        </li>
        <div id="menuContainer">
+
                        <li class="dropdown1"><a class="down-scroll" href="#">TEAM</a>
 
+
                            <ul class="dropdown2">
<!-- This list is your menu, every list item is a menu button and nested listed become submenu buttons -->
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Team/Member">Member</a></li>
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Attributions">Attribution</a></li>
                                   
+
                                <li><a href="https://2015.igem.org/Team:OUC-China/Collaborations">Collaboration</a></li>
 
+
                            </ul>
                 <a href= "https://igem.org/Main_Page"><image class="button" src="https://static.igem.org/mediawiki/2015/5/52/HKUST-Rice15_iGEMofficiallogo.jpg" style="width: 82px; height: 72px; margin: -10px; position: absolute; Left: 1.5%; top: 20px;"></a></li>
+
                        </li>
+
                    </ul>
                 <a><image src="https://static.igem.org/mediawiki/2015/4/48/HKUST-Rice15_wikilogo.jpg" style="width: 72px; height: 72px; margin: -10px; position: absolute; Left: 9.5%; top: 20px;"></a></li>                                                                      
+
                    <script>
 
+
                    $("span.menu").click(function() {
                 <ul>
+
                        $(" ul.nav").slideToggle("slow", function() {});
                                         
+
                    });
                <li><a href="https://2015.igem.org/Team:HKUST-Rice" style=" margin-top:10px;">HOME</a></li>
+
                    </script>
 
+
                 </div>
                 <li><a style= "background-color: white; color: black; font-weight: bold; margin-top:10px;" href="https://2015.igem.org/Team:HKUST-Rice/Description">DESCRIPTION</a>
+
                <div class="clearfix"> </div>
                <ul><li><a href="https://2015.igem.org/Team:HKUST-Rice/Description">OVERVIEW</a></li>
+
            </div>
 
+
            <!-- /.container-fluid -->
                <li><a>NITRATE</a>              
+
        </nav>
<ul>
+
    </header>
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Design">DESIGN</a></li>
+
    <div class="banner-container">
 
+
        <div class="banner-content">
        <li><a href="https://2015.igem.org/Team:HKUST-Rice/Experiments">EXPERIMENT</a></li>
+
            <img src="https://static.igem.org/mediawiki/2015/4/4b/OUC-China-Team_Logo.png" width="400" alt="" class="img-responsive center-block">
+
            <h2>Magthermo coli</h2>
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Modeling">MODELING</a></li>
+
        </div>
+
    </div>
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Results">RESULT</a></li>                                    
+
    <div class="container-fluid how-work">
</ul>  
+
        <div class="container">
 +
            <div class="work-header">
 +
                 <h2 class="text-uppercase">
 +
                    how does it <span>work?</span>
 +
                </h2>
 +
                <p>
 +
                    This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.
 +
                </p>
 +
            </div>
 +
            <div class="row work-content">
 +
                <div class="col-md-4">
 +
                    <img src="https://static.igem.org/mediawiki/2015/e/e1/OUC-China-home_receiver_regulator1.png" alt="" class="img-responsive">
 +
                    <h3 class="text-uppercase text-center">step 1</h3>
 +
                    <p>Our Magthermo coli contains 2 core components: <B>Magnetic Receiver & Thermosensitive Regulator.</B></p>
 +
                </div>
 +
                <div class="col-md-4">
 +
                    <img src="https://static.igem.org/mediawiki/2015/e/e7/OUC-China-home_receiver_regulator2.png" alt="" class="img-responsive">
 +
                    <h3 class="text-uppercase text-center">step 2</h3>
 +
                    <p>Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.</p>
 +
                </div>
 +
                 <div class="col-md-4">
 +
                    <img src="https://static.igem.org/mediawiki/2015/6/6d/OUC-China_home_receiver_regulator3.png" alt="" class="img-responsive">
 +
                    <h3 class="text-uppercase text-center">step 3</h3>
 +
                    <p>
 +
                        In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.
 +
                    </p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
    </div>
 +
    <div class="container-fluid index-details1 index-details">
 +
        <div class="container">
 +
            <h3 class="text-center index-headline">Magnetic <span>Receiver</span></h3>
 +
            <div class="row">
 +
                 <div class="col-md-7">
 +
                    <div class="caption">
 +
                        <p>
 +
                            For magnetic receiver,we chose <B>Ferritin</B>, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.
 +
                        </p>
 +
                        <p>
 +
                            In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.
 +
                        </p>
 +
                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Magnetic_Receiver" class="btn btn-primary" role="button">More details</a></p>
 +
                    </div>
 +
                 </div>
 +
                <div class="col-md-5">
 +
                    <img src="https://static.igem.org/mediawiki/2015/1/17/OUC-China-home-frritin.png" alt="" class="img-responsive">
 +
                </div>
 +
            </div>
 +
        </div>
 +
    </div>
 +
    <div class="container-fluid index-details2 index-details">
 +
        <div class="container">
 +
            <h3 class="text-center index-headline index-headline2">Thermosensitive <span>Regulator</span></h3>
 +
            <p class="text-center">For thermosensitive regulator,we chose <B>RNA thermometer</B> and designed a <B>thermosensitive T7 RNA polymerase.</B>  </p>
 +
            <div class="row">
 +
                <div class="col-md-6">
 +
                    <img src="https://static.igem.org/mediawiki/2015/b/b8/OUC-China-home-RNA.png" alt="" class="img-responsive">
 +
                </div>
 +
                <div class="col-md-6">
 +
                    <div class="caption">
 +
                        <p>
 +
                            <B>RNA thermometer</B> is a structured RNA which can expose SD sequences only at appropriate temperature.  In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
 +
                        </p>
 +
                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
            <div class="row">
 +
                <div class="col-md-6">
 +
                    <div class="caption">
 +
                        <p>
 +
                            <B>Thermosensitive T7 RNA polymerase</B> is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.
 +
                        </p>
 +
                        <p><a href="https://2015.igem.org/Team:OUC-China/Project/Thermosensitive_Regulator" class="btn btn-primary" role="button">More details</a></p>
 +
                    </div>
 +
                </div>
 +
                <div class="col-md-6">
 +
                    <img src="https://static.igem.org/mediawiki/2015/4/4d/OUC-China-home-T7.png" alt="" class="img-responsive">
 +
                </div>
 +
            </div>
 +
        </div>
 +
    </div>
 +
    <div class="returnTop">
 +
        <img src="https://static.igem.org/mediawiki/2015/a/a4/ReturnTop.png" alt="">
 +
    </div>
 +
    <footer>
 +
        <div class="container">
 +
            <address class="center-block">
 +
                Follow us on Facebook@ <a href="https://www.facebook.com/oucigem">iGEM OUC</a>
 +
                <br>Mail to: <a href="mailto:#">oucigem@163.com</a>
 +
            </address>
 +
            <center><ul class="footer-img">
 +
                <li>
 +
                    <img src="https://static.igem.org/mediawiki/2015/2/27/OUC-China_footer1.png" alt="" class="img-responsive">
 
                 </li>
 
                 </li>
 
+
                 <li>
                 <li><a>PHOSPHATE</a>
+
                    <img src="https://static.igem.org/mediawiki/2015/7/7c/OUC-China_footer2.png" alt="" class="img-responsive">
<ul>
+
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Design">DESIGN</a></li>
+
 
+
        <li><a href="https://2015.igem.org/Team:HKUST-Rice/Experiments">EXPERIMENT</a></li>
+
+
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Modeling">MODELING</a></li>
+
+
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Results">RESULT</a></li>                                    
+
</ul>  
+
 
                 </li>
 
                 </li>
 
+
                 <li>
                 <li><a>POTASSIUM</a>
+
                    <a href="http://www.genewiz.com/"><img src="https://static.igem.org/mediawiki/2015/0/08/OUC-China_footer3.png" alt="" class="img-responsive"></a>
<ul>
+
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Design">DESIGN</a></li>
+
 
+
        <li><a href="https://2015.igem.org/Team:HKUST-Rice/Experiments">EXPERIMENT</a></li>
+
+
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Modeling">MODELING</a></li>
+
+
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Results">RESULT</a></li>                                    
+
</ul>  
+
 
                 </li>
 
                 </li>
 
+
                 <li>
                 <li><a>EXPRESSION</a>
+
                    <img src="https://static.igem.org/mediawiki/2015/2/26/OUC-China_footer4.png" alt="" class="img-responsive">
<ul>
+
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Design">DESIGN</a></li>
+
 
+
        <li><a href="https://2015.igem.org/Team:HKUST-Rice/Experiments">EXPERIMENT</a></li>
+
+
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Modeling">MODELING</a></li>
+
+
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Results">RESULT</a></li>                                    
+
</ul>
+
                </li>                                     
+
 
+
                </ul>
+
 
                 </li>
 
                 </li>
 
+
                <li>
            <!-- <ul>
+
                    <img src="https://static.igem.org/mediawiki/2015/3/3e/OUC-China_footer5.png" alt="" class="img-responsive">
<a href="https://2015.igem.org/Team:HKUST-Rice/Description"><li>Description</li></a>
+
<a href="https://2015.igem.org/Team:HKUST-Rice/Experiments"><li>Experiments &amp; Protocols</li></a> 
+
<a href="https://2015.igem.org/Team:HKUST-Rice/Results"><li>Results</li></a> 
+
<a href="https://2015.igem.org/Team:HKUST-Rice/Design"><li>Design</li></a>
+
</ul> -->
+
 
+
                <li><a class="navItem" href style=" margin-top:10px;">RESULTS</a>
+
            <ul>
+
<li><a>NITRATE
+
                </a>
+
        <!-- <ul>
+
                <li><a>NITRATE
+
        </a></li>
+
 
+
        <li><a>PHOSPHATE
+
</a></li>
+
 
+
<li><a>POTASSIUM
+
</a></li>
+
 
+
<li><a>EXPRESSION
+
</a></li>                                     
+
 
+
</ul> -->
+
</li>
+
 
+
                <li><a>PHOSPHATE
+
                </a></li>
+
 
+
                <li><a>POTASSIUM
+
                </a></li>
+
 
+
                <li><a>EXPRESSION
+
                </a></li>                                     
+
 
+
                </ul>
+
                </li>
+
 
+
                                     
+
        <li><a class="navItem" href style=" margin-top:10px;">ACHIEVEMENT</a>
+
                <ul>
+
                <li><a>PARTS</a></li>
+
                <li><a>MEDAL REQUIREMENT</a></li>
+
                </ul>
+
                </li>
+
 
+
 
+
                <!-- <ul>
+
<a href="https://2015.igem.org/Team:HKUST-Rice/Parts"><li>Team Parts</li></a>
+
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Basic_Part">BASIC</a></li> 
+
<li><a href="https://2015.igem.org/Team:HKUST-Rice/Composite_Part">COMPOSITE</a></li>
+
        <a href="https://2015.igem.org/Team:HKUST-Rice/Part_Collection"><li>Part Collection</li></a>
+
</ul> -->
+
 
+
 
+
                <li><a class="navItem" href style=" margin-top:10px;">TEAM</a>
+
                <ul>
+
                <li><a href="https://2015.igem.org/Team:HKUST-Rice/Collaborations">COLLABORATION</a></li>
+
                <li><a href="https://2015.igem.org/Team:HKUST-Rice/Attributions">ATTRIBUTION</a></li>
+
                <li><a href="https://2015.igem.org/Team:HKUST-Rice/Team">MEMBERS</a></li>
+
                <li><a href="https://2015.igem.org/Team:HKUST-Rice/Advisors">ADVISORS</a></li>
+
                <li><a href="https://2015.igem.org/Team:HKUST-Rice/Instructors">INSTRUCTORS</a></li>
+
                <!-- <ul><li>TEAM</li>
+
                <li>ADVISORS</li>
+
                <li>INSTRUCTORS</li></ul> -->
+
                                                       
+
                </ul>
+
 
                 </li>
 
                 </li>
 
+
                 <div class="clr"></div>
 
+
             </ul></center>
     
+
         </div>   
<li><a class="navItem" href style=" margin-top:10px;">HUMAN PRACTICE</a>
+
    </footer>
                <ul><li><a href="https://2015.igem.org/Team:HKUST-Rice/Practices">INTRODUCTION</a></li>
+
                <li><a href="https://2015.igem.org/Team:HKUST-Rice/Practices_Debate">DEBATE</a></li>
+
                <li><a href="https://2015.igem.org/Team:HKUST-Rice/Practices_EvaluationPaper">EXPLORATORY RESEARCH</a></li>
+
                <li><a href="https://2015.igem.org/Team:HKUST-Rice/Practices_Analysis">ANALYSIS</a></li>
+
                </ul>
+
                </li>
+
 
+
        <li><a class="navItem" href style=" margin-top:10px;">WETLAB</a>
+
                <ul><li><a href="https://2015.igem.org/Team:HKUST-Rice/Notebook">NOTEBOOK</a></li>
+
                <li><a href="https://2015.igem.org/Team:HKUST-Rice/Protocol">PROTOCOL</a></li>
+
                <li><a href="https://2015.igem.org/Team:HKUST-Rice/Safety">SAFETY</a>
+
                <!--  <ul><li><a>DESIGN</a></li>
+
                <li><a>LAB WORK</a></li>
+
                <li><a>SHIPMENT</a></li></ul> -->
+
                </li>
+
                </ul>
+
                </li>
+
 
+
<!-- <a href="https://2015.igem.org/Team:HKUST-Rice/Modeling"><li>MODELING</li></a>
+
 
+
                <a href="https://2015.igem.org/Team:HKUST-Rice/Measurement"><li>MEASUREMENT</li></a>
+
 
+
<a href="https://2015.igem.org/Team:HKUST-Rice/Software"><li>SOFTWARE</li></a>
+
 
+
                <a href="https://2015.igem.org/Team:HKUST-Rice/Entrepreneurship"><li>ENTREPRENEURSHIP</li></a> -->
+
 
+
 
+
 
+
                <li><a class="navItem" href style=" margin-top:10px;">FUTURE WORK</a>
+
                <ul><li><a>APPLICATION</a></li>
+
                <li><a>IMPROVEMENT</a></li></ul>
+
                </li>
+
 
+
</ul>      
+
+
                           
+
                <a href= "http://www.ust.hk"><image class="button" src="https://static.igem.org/mediawiki/2015/3/3b/Team-HKUST-Rice-Hkust_logo_white.jpg" style="width: 51px; height: 72px; margin: -10px; position: absolute; Left: 85%; top: 20px;"></a>
+
                           
+
                <a href= "http://www.rice.edu/"><image class="button" src="https://static.igem.org/mediawiki/2015/4/46/HKUST-Rice15_ricelogo2.jpg" style="width: 106px; height: 40px; margin: -10px; position: absolute; Left: 90%; top: 40px;"></a>
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+
  <!-- End of menu  -->
+
 
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+
<head>
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                 <style type="text/css">
+
#contentContainer {
+
padding-top:20px;
+
                                padding-bottom: 20px;
+
padding-right:20px;
+
                                padding-left: 20px;
+
margin-bottom: 10px;
+
                                margin-left: 20px;
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                                margin-right: 20px;
+
width: 92%;
+
float: left;
+
background-color: #FFFFFF;
+
font-family: "Trebuchet MS", Helvetica, sans-serif;
+
}
+
 
+
p.link { text-align: right; font-size: 130%;}
+
a.learn:link{ text-decoration: none;}
+
p.link:hover {font-size: 150%;}
+
p {text-align: justify;}
+
 
+
#quicklink {
+
padding-top:20px;
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                                padding-bottom: 20px;
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padding-right:0px;
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                                padding-left: 0px;
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margin-bottom: 0px;
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                                margin-left: 0px;
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                                margin-right: 0px;
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width: 100%;
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                                height: 450px;
+
float: left;
+
background-color: #1b1c3a;
+
font-family: "Trebuchet MS", Helvetica, sans-serif;
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}
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+
img.button {
+
    opacity: 1.0;
+
    filter: alpha(opacity=100); /* For IE8 and earlier */
+
}
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+
img.button:hover {
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    opacity: 0.7;
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    filter: alpha(opacity=80); /* For IE8 and earlier */
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}
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/*Set up height place holder for the banner*/
+
#bannerContainer {
+
                                width: 100%;
+
                                height:550px;
+
overflow:hidden;
+
margin: 0px;
+
text-align:center;
+
}
+
 
+
 
+
                        #Directory { 
+
width: 100%;
+
overflow:hidden;
+
float:left;
+
margin-left: 0px;
+
                                margin-right: 0px;
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margin-bottom: 0px;
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border-bottom: 0px solid #565656;
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border-right: 0px solid #565656;
+
border-left: 0px solid #565656;
+
border-top: 0px solid #565656;
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font-family: "Trebuchet MS", Helvetica, sans-serif;
+
}               
+
                          /*Style the Directory*/     
+
                          #Directory table {
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+
 
+
<p style="font-size:40px;color:#FFFFFF;line-height:1em;">  Potassium, Nitrate and Phosphate Biosensor </font><br>
+
                                <font size= "5"> HKUST-Rice iGEM Team </font></p>
+
             
+
+
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         </div>
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<br>
+
<div id="contentContainer"><p><font size= "6" color=#6B6B47>ABSTRACT</font></p>
+
<br>
+
<p><font size= "4" color=#6B6B47>Nitrogen (N), Phosphorus (P), and potassium (K) are three macronutrients for plants, and deficiencies in any of these can lead to plant diseases. By creating a biological sensor that can quickly provide soil status to plant owners, we can prevent plant diseases due to the lack of nutrients.
+
<br><br>
+
In the previous iGEM competitions, multiple teams have worked on nitrate and phosphate responsive promoters, but none of them attempted to provide a potassium responsive promoter. In view of this, our team is constructing a biological sensor in <i>E. coli</i>, which can detect NPK levels in the surrounding environment and give responses in the form of colors. In addition, we are characterizing the effects of a dual output system, in contrast to a single output system, in order to anticipate the expression of multiple outputs in a single system.</font></p>
+
<br>
+
  <p class="link"><a class=" learn" href><font color=#6B6B47> Learn more ... </font></a></p>
+
</div>
+
 
+
 
+
<!--
+
<h3><ins>Background and Significance</ins></h3>
+
</div>
+
 
+
<h3> Welcome to iGEM 2015! </h3>
+
<p><h4>Your team has been approved and you are ready to start the iGEM season!</h4></p>
+
 
+
<h4>Before you start: </h4>
+
<p><h4>Please read the following pages:</h4></p>
+
<ul>
+
<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
+
<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
+
</ul>
+
 
+
<div class="highlightBox">
+
<h4> Styling your wiki </h4>
+
<p><h4>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</h4></p>
+
<p><h4>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</h4></p>
+
</div>
+
 
+
<h4> Editing your wiki </h4>
+
<p><h4>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!</h4></p>
+
<p><h4> <a href="https://2015.igem.org/wiki/index.php?title=Team:HKUST-Rice&amp;action=edit"> Click here to edit this page! </a></h4></p>
+
<p><h4>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</h4></p>
+
 
+
 
+
<h4>Templates </h4>
+
<p><h4>This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
+
<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</h4></p>
+
 
+
 
+
<h4>Tips</h4>
+
<p><h4>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:</h4></p>
+
<ul>
+
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
<li>Be clear about what you are doing and how you plan to do this.</li>
+
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
+
<li>Have lots of fun! </li>
+
</ul>
+
 
+
 
+
<h4>Inspiration</h4>
+
<p><h4> You can also view other team wikis for inspiration! Here are some examples:</h4></p>
+
<ul>
+
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
</ul>
+
 
+
<h4> Uploading pictures and files </h4>
+
<p> <h4>You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
+
When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</h4></p>
+
 
+
<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
+
-->
+
 
+
 
+
 
+
 
+
 
+
<div id="quicklink">
+
<a href= "#"><image class="button" src="https://static.igem.org/mediawiki/2015/f/fe/HKUST-Rice15_Ksensorbutton.jpg" style="width: 30%; height: 220px; position: absolute; left: 3%"></a>
+
 
+
<a href= "#"><image class="button" src="https://static.igem.org/mediawiki/2015/e/e1/HKUST-Rice15_Psensorbutton.jpg" style="width: 30%; height: 220px; position: absolute; left: 35%"></a>
+
 
+
<a href= "#"><image class="button" src="https://static.igem.org/mediawiki/2015/8/87/HKUST-Rice15_Nsensorbutton.jpg" style="width: 30%; height: 220px; position: absolute; left: 67%"></a>
+
<br><br><br><br><br><br><br><br><br><br><br><br><br>
+
 
+
<a href= "#"><image class="button" src="https://static.igem.org/mediawiki/2015/b/bf/Team-2015-HKUST-Rice-new_human_practice.png" style="width: 30%; height: 220px; position: absolute; left: 3%"></a>
+
 
+
<a href= "#"><image class="button" src="https://static.igem.org/mediawiki/2015/8/8b/HKUST-Rice15_Expressionbutton.png" style="width: 30%; height: 220px; position: absolute; left: 35%"></a>
+
 
+
<a href= "#"><image class="button" src="https://static.igem.org/mediawiki/2015/b/be/HKUST-Rice15_Resultsbutton.png" style="width: 30%; height: 220px; position: absolute; left: 67%"></a>
+
 
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</div>
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</div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
+
 
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+
<div id="Directory">
+
 
+
  <table>
+
             
+
<tr>
+
                    <th><a href= "https://2015.igem.org/Team:HKUST-Rice">Home</a></th>
+
                    <th colspan="3"><a href= "https://2015.igem.org/Team:HKUST-Rice" style="color:white">Project</a></th>
+
                    <th><a>Result</a></th>
+
                    <th><a>Achievement</a></th>
+
                    <th><a href= "https://2015.igem.org/Team:HKUST-Rice/Notebook">Team</a></th>
+
                    <th><a href= "https://2015.igem.org/Team:HKUST-Rice/Practices">Human Practice</a></th>
+
                    <th><a href= "https://2015.igem.org/Team:HKUST-Rice/Safety">Wetlab</a></th>
+
                    <th>Future Work</th>
+
                   
+
                   
+
                </tr>
+
 
+
 
+
<tr Style= "vertical-align: top">
+
                    <td><ul>
+
                    <!-- <li><a href="https://2015.igem.org/Team:HKUST-Rice">Project</a></li>
+
                    <li><a href"https://2015.igem.org/Team:HKUST-Rice/Team">Team</a></li>
+
                    <li>Parts</li>
+
                    <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Notebook">Notebook</a></li>
+
                    <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Practices">Human Practice</a></li>
+
                    <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Safety">Safety</a></li>
+
                    <li>Future Work</li>
+
                    <li>Achievement</li> -->
+
                    </ul></td>
+
 
+
+
                 
+
                    <td><ul>
+
                    <li>Overview</li>
+
                    <li>Nitrate
+
                        <ul>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Description">Description</a></li>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Design">Design</a></li>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Experiments">Experiments</a></li> 
+
                        <li>Modelling</li>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Results">Results</a></li>
+
                        <li>Discussion</li>
+
                        </ul>
+
                    </li>
+
                    </ul></td>
+
 
+
                    <td><ul>
+
                        <li>Phosphate
+
                        <ul>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Description">Description</a></li>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Design">Design</a></li>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Experiments">Experiments</a></li> 
+
                        <li>Modelling</li>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Results">Results</a></li>
+
                        <li>Discussion</li>
+
                        </ul>
+
                        </li>
+
                    </ul></td>
+
 
+
                    <td><ul>
+
                        <li>Potassium
+
                        <ul>
+
                        <li><a href="https://2015.igem.org/Team:HKUST-Rice/Description">Description</a></li>
+
                        <li><a href="https://2015.igem.org/Team:HKUST-Rice/Design">Design</a></li>
+
                        <li><a href="https://2015.igem.org/Team:HKUST-Rice/Experiments">Experiments</a></li> 
+
                        <li>Modelling</li>
+
                        <li><a href="https://2015.igem.org/Team:HKUST-Rice/Results">Results</a></li>
+
                        <li>Discussion</li>
+
                        </ul>
+
                        </li>
+
                    </ul></td>
+
 
+
 
+
                    <td><ul>
+
                        <li><a href= "#">Nitrate</a></li>
+
                        <li><a href= "#">Phosphate</a></li>
+
                        <li><a href= "#">Potassium</a></li>
+
                        <li><a href= "#">Expression</a></li>
+
                    </ul></td>
+
 
+
 
+
                    <td><ul>
+
                        <li><a href= "#">Parts</a></li>
+
                        <li><a href= "#">Medal Requirement</a></li>
+
                    </ul></td>
+
 
+
 
+
                    <td><ul>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Collaborations">Collaboration</a></li>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Attributions">Attribution</a></li>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Team">Team Members</a></li>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Team">Advisors</a></li>
+
                        <li><a href= "https://2015.igem.org/Team:HKUST-Rice/Team">Instructors</a></li>
+
 
+
                    </ul></td>
+
 
+
 
+
                    <td><ul>
+
                        <li>Debate</li>
+
                        <li>Exploratory Research</li>
+
                    </ul></td>
+
 
+
                 
+
                    <td><ul>
+
                        <li>Notebook</li>
+
                        <li>Protocol</li>
+
                        <li>Safety</li>
+
                    </ul></td>
+
 
+
 
+
              <!--      <td><ul>
+
                        <li>Basic</li>
+
                        <li>Composite</li>
+
                    </ul></td>
+
 
+
                    <td><ul>
+
                        <li>Design</li>
+
                        <li>Labwork</li>
+
                        <li>Shipment</li>
+
                    </ul></td>      -->
+
 
+
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Latest revision as of 01:50, 19 September 2015

<!DOCTYPE html> Team:OUC-China

how does it work?

This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.

step 1

Our Magthermo coli contains 2 core components: Magnetic Receiver & Thermosensitive Regulator.

step 2

Inducing with electromagnetic field, magnetic receiver will heat, raising the ambient temperature.

step 3

In response to the change of temperature, thermosensitive regulator will initiate downstream gene (GFP for example) expression.

Magnetic Receiver

For magnetic receiver,we chose Ferritin, the iron-storage protein in many organisms, which could synthesize ferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core will heat, raising the ambient temperature.

In this section, we explored measurement techniques for the valuation of magnetism, biomineralization and protein concentration per cell.

More details

Thermosensitive Regulator

For thermosensitive regulator,we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.

RNA thermometer is a structured RNA which can expose SD sequences only at appropriate temperature. In this section, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.

More details

Thermosensitive T7 RNA polymerase is a T7 RNA polymerase interrupted by a temperature-sensitive intein, which can self-splice at specific temperature and initiate the downstream signal.

More details