Difference between revisions of "Team:OLS Canmore AB CA"

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{{OLS_Canmore_AB_CA}}
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<h2> Welcome to iGEM 2015! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h4>Before you start: </h4>
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<!-- Declare that you are going to use html code instead of wiki code -->
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<p> Please read the following pages:</p>
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<html>
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<ul>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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</ul>
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<h4> Styling your wiki </h4>
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<style type="text/css">
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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</div>
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<h4> Editing your wiki </h4>
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@import url(font-awesome.min.css);
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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        @import url("http://fonts.googleapis.com/css?family=Roboto:500,300");
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:OLS_Canmore_AB_CA&action=edit"> Click here to edit this page! </a></p>
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
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<h4>Templates </h4>
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/* since the background is dark, we change the color of the text and links so they are visible on "show preview" mode */
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>
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<h4>Tips</h4>
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a, a:visited {
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>
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<h4>Inspiration</h4>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
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</ul>
 
  
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<h4> Uploading pictures and files </h4>
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
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<div id ="mainContainer"><!--This div will close on the page.-->
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<div id="bannerContainer">
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<img src="https://static.igem.org/mediawiki/2015/4/43/Team_OLS_Canmore_AB_CA_Banner.jpeg" width:"980px" height:"200px"/>
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</div>
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<!-- Start of menu -->
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<div id="menuContainer">
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<!-- This list is your menu, every list item is a menu button and nested listed become submenu buttons -->
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<ul>
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA"><li>HOME</li></a>
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Team"><li>TEAM</li></a>
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<a href="#"><li>PROJECT
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            <ul>
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Description"><li>Description</li></a>
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Experiments"><li>Experiments & Protocols</li></a> 
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Results"><li>Results</li></a> 
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                                                        <a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Design"><li>Design</li></a> 
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</ul>
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</li></a>
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<a href="#"><li>PARTS
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            <ul>
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Parts"><li>Team Parts</li></a>
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Basic_Part"><li>Basic Parts</li></a> 
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Composite_Part"><li>Composite Parts</li></a>
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</ul>
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</li></a>
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Notebook"><li>NOTEBOOK</li></a>
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Attributions"><li>ATTRIBUTIONS</li></a>
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Collaborations"><li>COLLABORATIONS</li></a>
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Practices"><li>HUMAN PRACTICES</li></a>
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<a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Safety"><li>SAFETY</li></a>
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                                        <a href="https://2015.igem.org/Team:OLS_Canmore_AB_CA/Faith"><li>FAITH</li></a>
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                                <h2><center><b>The BreaKERs</b></center></h2>
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<table width="792" border="0" cellpadding="0">
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      <td height="168"><p>We are the &quot;Our Lady of The Snows Catholic Academy - OLS&quot; team from Canmore, Alberta, Canada. Our project aims to break down hair in wastewater treatment facilities, and to break down feather waste in the poultry industry. </p>
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<br>
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      <p>Our plan is to genetically engineer <i>E.coli</i> K12 to express Keratinase&mdash;an enzyme that breaks down the protein keratin, found in feathers and hair.  We will use the KeratinaseA (KERA) and KeratinaseUS (KERUS) genetic sequences found naturally in the <i>Bacillus</i> genera.  The KERA and KERUS sequences will be optimized for expression in <i>E.coli</i>, synthesized into plasmid rings, and ligated into a standard biobrick backbone for submission to the iGEM parts registry.  It is from 'Keratinase' and 'keratin break-down' that we chose our name, The BreaKERs, and our motto, that we are KER-ate chopping the keratin waste.</p></td>
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    </tr>
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      <td height="322" align="center">      <img src="https://static.igem.org/mediawiki/2015/d/d5/OLShome_picture5.png" width="428" height="296" align="middle"/></td>
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      <td height="283"><p>After much brainstorming (above), we chose our project topic because our small community is struggling to deal with hair build-up in our wastewater treatment facilities.  These build-ups have caused equipment failure, clogs, and increased maintenance costs for our town.  We researched and found that the only current strategy being applied to these blockages is to manually remove the accumulated hair. We thought there must be a better way, using synthetic biology, to solve this problem.
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Through our research we also found that keratin waste is a huge issue in the poultry processing industry, as feathers are also made up of the protein keratin.  Billions of tonnes of feather wastes build-up, with limited options for disposal.  Currently, feathers are often being disposed of in environmentally harmful ways. One method is incineration, which leads to the release of many pollutants, foul odours, and harmful runoff contaminating livestock and plants in the surrounding area.  Burying the waste is another common disposal method, which leads to harmful leachates.  As keratin is protein-rich, there is an option of turning keratin waste into products such as animal feed or fertilizer; however this process is extremely extensive, expensive and time consuming, often producing products of low quality.<br>
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<br>
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Our project this year will provide an inexpensive and efficient method to more completely break down the keratin proteins in both hair and feathers, providing a step towards solving the issue of keratin waste treatment.</p></td>
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    </tr>
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      <td align="center"><img src="https://static.igem.org/mediawiki/2015/c/c9/OLShome_picture1.jpeg" width="750" height="500"/></td>
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                  </div>
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                        </div>
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<p font-color:"#fff"><center>Contact us at <strong>alinaarvisais@isidore.redeemer.ab.ca</strong> or <strong>taliadixon@isidore.redeemer.ab.ca</strong>, or even send a message to our facebook page: <a href="https://www.facebook.com/OLeSsence">https://www.facebook.com/OLeSsence</a></center><p>
 
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Latest revision as of 19:39, 30 October 2015

The BreaKERs

We are the "Our Lady of The Snows Catholic Academy - OLS" team from Canmore, Alberta, Canada. Our project aims to break down hair in wastewater treatment facilities, and to break down feather waste in the poultry industry.


Our plan is to genetically engineer E.coli K12 to express Keratinase—an enzyme that breaks down the protein keratin, found in feathers and hair. We will use the KeratinaseA (KERA) and KeratinaseUS (KERUS) genetic sequences found naturally in the Bacillus genera. The KERA and KERUS sequences will be optimized for expression in E.coli, synthesized into plasmid rings, and ligated into a standard biobrick backbone for submission to the iGEM parts registry. It is from 'Keratinase' and 'keratin break-down' that we chose our name, The BreaKERs, and our motto, that we are KER-ate chopping the keratin waste.

After much brainstorming (above), we chose our project topic because our small community is struggling to deal with hair build-up in our wastewater treatment facilities. These build-ups have caused equipment failure, clogs, and increased maintenance costs for our town. We researched and found that the only current strategy being applied to these blockages is to manually remove the accumulated hair. We thought there must be a better way, using synthetic biology, to solve this problem. Through our research we also found that keratin waste is a huge issue in the poultry processing industry, as feathers are also made up of the protein keratin. Billions of tonnes of feather wastes build-up, with limited options for disposal. Currently, feathers are often being disposed of in environmentally harmful ways. One method is incineration, which leads to the release of many pollutants, foul odours, and harmful runoff contaminating livestock and plants in the surrounding area. Burying the waste is another common disposal method, which leads to harmful leachates. As keratin is protein-rich, there is an option of turning keratin waste into products such as animal feed or fertilizer; however this process is extremely extensive, expensive and time consuming, often producing products of low quality.

Our project this year will provide an inexpensive and efficient method to more completely break down the keratin proteins in both hair and feathers, providing a step towards solving the issue of keratin waste treatment.

Contact us at alinaarvisais@isidore.redeemer.ab.ca or taliadixon@isidore.redeemer.ab.ca, or even send a message to our facebook page: https://www.facebook.com/OLeSsence