Team:OLS Canmore AB CA/Design
Design
Our team will create two different keratinase-expressing E. coli systems. One will express Keratinase A (KERA), an enzyme optimized to degrade hair, and the second will express Keratinase US (KERUS). |
The KERA and KERUS sequences will be optimized for expression in E. coli to prevent the misfolding of the protein in the periplasm, and allow for the export tag to be cleaved so the protein can leave the cell. The composite part will be synthesized into standard biobrick form with an RFP vector (part BBa_J04450), for submission to the iGem parts registry. An IPTG-inducible promoter (part BBa_J04500) from the standard registry of parts will then be added to each KER gene create an expression cassette for this protein, also for submission to the iGem parts registry. We will then work to characterize our genes and optimize protein expression and function. |
Plan Part 1 - Creating Basic Coding Sequence Biobricks:
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Plan Part 2: Creatin KerA/US Expression Cassette Biobricks
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