Difference between revisions of "Team:Georgia State"

 
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<html>
  
<html>
+
<head>
<style type="text/css">
+
    <meta charset="utf-8" />
@charset "UTF-8";
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    <title>IGEM Team:GSU</title>
@import url(font-awesome.min.css);
+
    <meta name="description" content="" />
@import url("http://fonts.googleapis.com/css?family=Roboto:500,300");
+
    <meta name="keywords" content="" />
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    <meta name="author" content="nileforest">
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    <meta name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.0" />
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    <!-- Favicone Icon -->
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 +
    <link rel="icon" type="image/png" href="img/favicon.png">
 +
    <link rel="apple-touch-icon" href="img/favicon.png">
  
body {
+
    <!-- CSS -->
background: #fff;
+
    <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Georgia_State/CSS?action=raw&ctype=text/css" />
}
+
<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Georgia_State/CSS1?action=raw&ctype=text/css" />
 +
</head>
  
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        <!-- HEADER -->
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 +
            <div class="container">
  
strong, b {
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                <!-- logo -->
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                <div class="logo">
font-weight: 500;
+
                    <a href="https://2015.igem.org/Team:Georgia_State">
}
+
                        <img style="margin-top: -10px;" class="l-black" src="https://static.igem.org/mediawiki/2015/a/a8/LogoBlack_GSUiGem.png" />
 +
                    </a>
 +
                </div>
 +
                <!--End logo-->
  
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font-style: italic;
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p {
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<!-- Navigation Menu -->
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+
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}
+
                    <ul>
 +
                        <li>
 +
                            <a href="https://2015.igem.org/Team:Georgia_State/Team">Team</a>
 +
                        </li>
 +
 +
                        <li>
 +
                            <a href="https://2015.igem.org/Team:Georgia_State/Project">Project</a>
 +
<ul>
 +
<li>
 +
  <a href="https://2015.igem.org/Team:Georgia_State/Project#Thing1">Mambalgin</a>
 +
</li>
 +
<li>
 +
  <a href="https://2015.igem.org/Team:Georgia_State/Project#Thing2">CBDA Synthase</a>
  
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+
</li>
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<li>
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+
  <a href="https://2015.igem.org/Team:Georgia_State/Parts">Parts Submitted</a>
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+
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+
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</li>
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+
  <a href="https://2015.igem.org/Team:Georgia_State/Description">Part Improvement</a>
}
+
  
h2 {
+
</li>
font-size: 1.5em;
+
</ul>
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+
<li> <a href="https://2015.igem.org/Team:Georgia_State/Notebook">Notebook</a>
}
+
</li>
  
h3 {
+
<li> <a href="https://2015.igem.org/Team:Georgia_State/Practices">Practices</a>
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+
<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing1">Atlanta Science Festival</a>
 +
</li>
 +
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing2">Six Flags Education Day</a>
 +
</li>
 +
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing3">Legislative Outreach</a>
 +
</li>
 +
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing4">Atlanta Botanical Gardens</a>
 +
</li>
 +
</ul>
 +
<li> <a href="https://2015.igem.org/Team:Georgia_State/Collaborations">Collaborations</a>
 +
<ul>
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Collaborations#ASF">Atlanta Science Festival</a>
 +
</li>
 +
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Collaborations#Thing2">Project JBroth</a>
 +
</li>
 +
</ul>
  
h4 {
+
<li> <a href="https://2015.igem.org/Team:Georgia_State/Safety">Safety</a>
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+
                        </li>
line-height: 1.5em;
+
<li> <a href="https://2015.igem.org/Team:Georgia_State/Attributions">Attributions</a>
}
+
</li> 
 +
                        <li> <a href="https://2015.igem.org/Team:Georgia_State/ImageGallery">Images</a>
 +
                        </li>
 +
                   
 +
                     
 +
                        <li>
 +
                            <a href="https://2015.igem.org/Team:Georgia_State#contact-info">Contact</a>
 +
                        </li>
  
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+
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        <!-- END HEADER -->
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}
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            <div class="media-wapper bg-img parallax parallax-section1 overlay-dark" style="top: 250px; background-image: url(https://static.igem.org/mediawiki/2015/9/9a/GSUiGEM_AtlantaSkyline.jpg);">
margin: 3em 0;
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 +
                    <div class="container">
 +
                        <br>
 +
                      <h2 class="h1">P<sup>4</sup>= Protein Products Pichia Plants</h2>
 +
                        <br>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
        </section>
 +
        <div class="clearfix"></div>
 +
        <!-- End Intro Section -->
  
hr.major {
+
<!--About Section-->
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        <section id="about" class="wow fadeIn ptb ptb-sm-80" style="background-color: #0387A6;">
}
+
            <div class="container">
 +
                <div class="row text-center">
 +
                    <div class="col-md-12">
 +
                        <h3 class="h4" style="text-align: center;color: white;">Our Project</h3>
 +
                        <div class="spacer-15"></div>
 +
                        <p class="lead"  style="color: white;" ><b> Cannabinoids and opiates are widely used classes of pharmaceuticals; unfortunately, these drugs have strong psychoactive effects or can be addictive. Our project consists of two ideas, both revolving around utilizing bioengineered microorganisms to create non-psychoactive cannabinoids and non-addictive analgesics. To achieve this we developed two projects: (1) Manufacturing a protein expression system to produce CBDA synthase in tobacco plants using agrobacterium, (2) Engineering the pGAPα vector system to express the mambalgin in Pichia Pastoris as a continuation of the 2014 GSU iGEM project. Simultaneously, we developed a proof of concept using horseradish peroxidase. By the end of this project we hope to have produced a synthetic biological system to manufacture pharmaceutical alternatives for patients that suffer from diseases such as epilepsy, cancer, or chronic severe pain.</p>
 +
                    </div>
 +
                </div>
 +
                   
 +
            </div>
 +
        </section>
 +
        <!-- End About Section-->
 +
        <hr />
  
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code {
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        <!-- Client Logos Section -->
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border-radius: 6px;
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            <div class="container text-center">
border: solid 1px rgba(144, 144, 144, 0.25);
+
                <h3>Sponsors </h3>
font-family: "Courier New", monospace;
+
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            <div class="spacer-60"></div>
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                    <div class="item">
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                        <div class="client-logo">
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                            <img src="https://static.igem.org/mediawiki/2015/5/56/GSUiGEM_STEM_logo.PNG" alt="" />
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                        </div>
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                    </div>
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                    <div class="item">
 +
                        <div class="client-logo">
 +
                            <img src="https://static.igem.org/mediawiki/2015/8/8d/GSUiGEM_biologylogo.png" alt="" />
 +
                        </div>
 +
                    </div>
 +
                    <div class="item">
 +
                        <div class="client-logo">
 +
                            <img src="https://static.igem.org/mediawiki/2015/b/ba/Idt_logo2015.jpg" alt="" />
 +
                        </div>
 +
                    </div>
 +
                    <div class="item">
 +
                        <div class="client-logo">
 +
                            <img src="https://static.igem.org/mediawiki/2015/0/0d/EmoryLogoCSE_GSUiGEM.JPG" alt="" />
 +
                        </div>
 +
                    </div>
 +
                   
 +
                </div>
 +
            </div>
 +
        </section>
 +
        <!-- End Client Logos Section -->
  
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        <section id="social" class="gray-bg ptb ptb-sm-80">
font-family: "Courier New", monospace;
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                    <div class="col-md-8 col-md-offset-2">
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+
                        <h3>Connect With Us</h3>
}
+
                    </div>
 +
                </div>
 +
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 +
                                <div class="page-icon-top">
 +
    <a href="https://twitter.com/GSUiGEM">
 +
                     
 +
<img src="https://static.igem.org/mediawiki/2015/5/5f/GSUiGEM-2015Twitter.png">
 +
                        </div>
 +
                              <h6><a class="Link" target="_blank" href="https://twitter.com/GSUiGEM">Twitter</a></h6>
 +
                                </div>
 +
                               
 +
                         
 +
                            <div class="col-md-6 col-sm-4 col-xs-6">
 +
                                <div class="page-icon-top">
 +
<a href="https://www.facebook.com/GSUiGEM">
 +
<img src="https://static.igem.org/mediawiki/2015/9/95/GSUiGEM-fb.png">
 +
                                </div>
 +
                                <h6><a class="Link" target="_blank" href="https://www.facebook.com/GSUiGEM">Facebook</a></h6>
 +
                            </div>
 +
                           
 +
                        </div>
 +
    </div>
 +
                </div>
 +
            </div>
 +
        </section>
 +
        <!-- End Social Section -->
  
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                        <div class="contact-box-left">
 +
                            <div class="contact-icon-left">
 +
<img src="https://static.igem.org/mediawiki/2015/0/04/GSUiGEM-Location.png">
 +
</div>
 +
                            <h6>Address</h6>
 +
                            <p>
 +
                                iGEM Lab
 +
                                <br>
 +
                                24 Peachtree Center Ave SE,<br> Kell Hall 439<br>
 +
                                Atlanta, Georgia 30303
 +
                            </p>
  
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+
                        </div>
text-align: right;
+
                    </div>
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+
  
/* Section/Article */
+
                    <div class="col-md-4">
 +
                        <div class="contact-box-left">
 +
                            <div class="contact-icon-left">
 +
<img src="https://static.igem.org/mediawiki/2015/9/93/GSUiGEM-phone.png">
 +
</div>
 +
                            <h6>Call Us</h6>
 +
                            <p>
 +
                                <span>We're all millennials. We don't like talking on the phone. <a href="https://twitter.com/GSUiGEM">Send us a Tweet!</a></span><br />
 +
                            </p>
 +
                        </div>
 +
                    </div>
  
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                            <div class="contact-icon-left">
 +
<img src="https://static.igem.org/mediawiki/2015/d/d2/GSUiGEM-email.png">
 +
</div>
 +
                            <h6>Email</h6>
 +
                            <p>
 +
                                <a href="mailto:mbrewer@gsu.edu">mbrewer@gsu.edu</a>
 +
                            </p>
 +
                        </div>
 +
                    </div>
  
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                    <p class="">
}
+
                        &copy; 2015 <a><b>GSU IGEM</b></a>. All Rights Reserved.
 +
                        <br />
 +
                     
 +
                    </p>
 +
                </div>
 +
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 +
            <!-- End Copyright Bar -->
  
header h4 + p,
+
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header h5 + p,
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<img src="https://static.igem.org/mediawiki/2015/8/84/GSUiGEM-uparrow.png" alt="" />
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+
text-decoration: none;
+
width: 100%;
+
}
+
 
+
input[type="text"]:invalid,
+
input[type="password"]:invalid,
+
input[type="email"]:invalid,
+
select:invalid,
+
textarea:invalid {
+
box-shadow: none;
+
}
+
 
+
input[type="text"]:focus,
+
input[type="password"]:focus,
+
input[type="email"]:focus,
+
select:focus,
+
textarea:focus {
+
border-color: #629DD1;
+
box-shadow: 0 0 0 1px #629DD1;
+
}
+
 
+
.select-wrapper {
+
text-decoration: none;
+
display: block;
+
position: relative;
+
}
+
 
+
.select-wrapper:before {
+
content: "";
+
-moz-osx-font-smoothing: grayscale;
+
-webkit-font-smoothing: antialiased;
+
font-family: FontAwesome;
+
font-style: normal;
+
font-weight: normal;
+
text-transform: none !important;
+
}
+
 
+
.select-wrapper:before {
+
color: rgba(144, 144, 144, 0.25);
+
display: block;
+
height: 2.75em;
+
line-height: 2.75em;
+
pointer-events: none;
+
position: absolute;
+
right: 0;
+
text-align: center;
+
top: 0;
+
width: 2.75em;
+
}
+
 
+
.select-wrapper select::-ms-expand {
+
display: none;
+
}
+
 
+
input[type="text"],
+
input[type="password"],
+
input[type="email"],
+
select {
+
height: 2.75em;
+
}
+
 
+
textarea {
+
padding: 0.75em 1em;
+
}
+
 
+
input[type="checkbox"],
+
input[type="radio"] {
+
-moz-appearance: none;
+
-webkit-appearance: none;
+
-o-appearance: none;
+
-ms-appearance: none;
+
appearance: none;
+
display: block;
+
float: left;
+
margin-right: -2em;
+
opacity: 0;
+
width: 1em;
+
z-index: -1;
+
}
+
 
+
input[type="checkbox"] + label,
+
input[type="radio"] + label {
+
text-decoration: none;
+
color: #555f66;
+
cursor: pointer;
+
display: inline-block;
+
font-size: 1em;
+
font-weight: 300;
+
padding-left: 2.4em;
+
padding-right: 0.75em;
+
position: relative;
+
}
+
 
+
input[type="checkbox"] + label:before,
+
input[type="radio"] + label:before {
+
-moz-osx-font-smoothing: grayscale;
+
-webkit-font-smoothing: antialiased;
+
font-family: FontAwesome;
+
font-style: normal;
+
font-weight: normal;
+
text-transform: none !important;
+
}
+
 
+
input[type="checkbox"] + label:before,
+
input[type="radio"] + label:before {
+
background: rgba(144, 144, 144, 0.075);
+
border-radius: 6px;
+
border: solid 1px rgba(144, 144, 144, 0.25);
+
content: '';
+
display: inline-block;
+
height: 1.65em;
+
left: 0;
+
line-height: 1.58125em;
+
position: absolute;
+
text-align: center;
+
top: 0;
+
width: 1.65em;
+
}
+
 
+
input[type="checkbox"]:checked + label:before,
+
input[type="radio"]:checked + label:before {
+
background: #192024;
+
border-color: #192024;
+
color: #ffffff;
+
content: '\f00c';
+
}
+
 
+
input[type="checkbox"]:focus + label:before,
+
input[type="radio"]:focus + label:before {
+
border-color: #629DD1;
+
box-shadow: 0 0 0 1px #629DD1;
+
}
+
 
+
input[type="checkbox"] + label:before {
+
border-radius: 6px;
+
}
+
 
+
input[type="radio"] + label:before {
+
border-radius: 100%;
+
}
+
 
+
::-webkit-input-placeholder {
+
color: #bbb !important;
+
opacity: 1.0;
+
}
+
 
+
:-moz-placeholder {
+
color: #bbb !important;
+
opacity: 1.0;
+
}
+
 
+
::-moz-placeholder {
+
color: #bbb !important;
+
opacity: 1.0;
+
}
+
 
+
:-ms-input-placeholder {
+
color: #bbb !important;
+
opacity: 1.0;
+
}
+
 
+
.formerize-placeholder {
+
color: #bbb !important;
+
opacity: 1.0;
+
}
+
 
+
/* Box */
+
 
+
.box {
+
border-radius: 6px;
+
border: solid 1px rgba(144, 144, 144, 0.25);
+
margin-bottom: 2em;
+
padding: 3em 2em;
+
}
+
 
+
.box > :last-child,
+
.box > :last-child > :last-child,
+
.box > :last-child > :last-child > :last-child {
+
margin-bottom: 0;
+
}
+
 
+
.box.alt {
+
border: 0;
+
border-radius: 0;
+
padding: 0;
+
}
+
 
+
/* Icon */
+
 
+
.icon {
+
text-decoration: none;
+
border-bottom: none;
+
position: relative;
+
}
+
 
+
.icon:before {
+
-moz-osx-font-smoothing: grayscale;
+
-webkit-font-smoothing: antialiased;
+
font-family: FontAwesome;
+
font-style: normal;
+
font-weight: normal;
+
text-transform: none !important;
+
}
+
 
+
.icon > .label {
+
display: none;
+
}
+
 
+
.icon.major {
+
display: inline-block;
+
margin: 0 0 1.25em 0;
+
color: #ffffff;
+
background: #629DD1;
+
width: 3em;
+
height: 3em;
+
font-size: 2em;
+
text-align: center;
+
line-height: 3em;
+
border-radius: 100%;
+
box-shadow: 0 0 0 10px #fff, 0 0 0 11px rgba(144, 144, 144, 0.25);
+
}
+
 
+
/* Image */
+
 
+
.image {
+
border-radius: 6px;
+
border: 0;
+
display: inline-block;
+
position: relative;
+
}
+
 
+
.image img {
+
border-radius: 6px;
+
display: block;
+
}
+
 
+
.image.left {
+
float: left;
+
padding: 0 1.5em 1em 0;
+
top: 0.25em;
+
}
+
 
+
.image.right {
+
float: right;
+
padding: 0 0 1em 1.5em;
+
top: 0.25em;
+
}
+
 
+
.image.fit {
+
display: block;
+
margin: 0 0 2em 0;
+
width: 100%;
+
}
+
 
+
.image.fit img {
+
display: block;
+
width: 100%;
+
}
+
 
+
/* List */
+
 
+
ol {
+
list-style: decimal;
+
margin: 0 0 2em 0;
+
padding-left: 1.25em;
+
}
+
 
+
ol li {
+
padding-left: 0.25em;
+
}
+
 
+
ul {
+
list-style: disc;
+
margin: 0 0 2em 0;
+
padding-left: 1em;
+
}
+
 
+
ul li {
+
padding-left: 0.5em;
+
}
+
 
+
ul.alt {
+
list-style: none;
+
padding-left: 0;
+
}
+
 
+
ul.alt li {
+
border-top: solid 1px rgba(144, 144, 144, 0.25);
+
padding: 0.5em 0;
+
}
+
 
+
ul.alt li:first-child {
+
border-top: 0;
+
padding-top: 0;
+
}
+
 
+
ul.icons {
+
cursor: default;
+
list-style: none;
+
padding-left: 0;
+
}
+
 
+
ul.icons li {
+
display: inline-block;
+
padding: 0 1.25em 0 0;
+
}
+
 
+
ul.icons li:last-child {
+
padding-right: 0;
+
}
+
 
+
ul.icons li .icon:before {
+
font-size: 1.5em;
+
}
+
 
+
ul.actions {
+
cursor: default;
+
list-style: none;
+
padding-left: 0;
+
}
+
 
+
ul.actions li {
+
display: inline-block;
+
padding: 0 1em 0 0;
+
vertical-align: middle;
+
}
+
 
+
ul.actions li:last-child {
+
padding-right: 0;
+
}
+
 
+
ul.actions.small li {
+
padding: 0 0.5em 0 0;
+
}
+
 
+
ul.actions.vertical li {
+
display: block;
+
padding: 1em 0 0 0;
+
}
+
 
+
ul.actions.vertical li:first-child {
+
padding-top: 0;
+
}
+
 
+
ul.actions.vertical li > * {
+
margin-bottom: 0;
+
}
+
 
+
ul.actions.vertical.small li {
+
padding: 0.5em 0 0 0;
+
}
+
 
+
ul.actions.vertical.small li:first-child {
+
padding-top: 0;
+
}
+
 
+
ul.actions.fit {
+
display: table;
+
margin-left: -1em;
+
padding: 0;
+
table-layout: fixed;
+
width: calc(100% + 1em);
+
}
+
 
+
ul.actions.fit li {
+
display: table-cell;
+
padding: 0 0 0 1em;
+
}
+
 
+
ul.actions.fit li > * {
+
margin-bottom: 0;
+
}
+
 
+
ul.actions.fit.small {
+
margin-left: -0.5em;
+
width: calc(100% + 0.5em);
+
}
+
 
+
ul.actions.fit.small li {
+
padding: 0 0 0 0.5em;
+
}
+
 
+
dl {
+
margin: 0 0 2em 0;
+
}
+
 
+
/* Table */
+
 
+
.table-wrapper {
+
-webkit-overflow-scrolling: touch;
+
overflow-x: auto;
+
}
+
 
+
table {
+
margin: 0 0 2em 0;
+
width: 100%;
+
}
+
 
+
table tbody tr {
+
border: solid 1px rgba(144, 144, 144, 0.25);
+
border-left: 0;
+
border-right: 0;
+
}
+
 
+
table tbody tr:nth-child(2n + 1) {
+
background-color: rgba(144, 144, 144, 0.075);
+
}
+
 
+
table td {
+
padding: 0.75em 0.75em;
+
}
+
 
+
table th {
+
color: #666f77;
+
font-size: 0.9em;
+
font-weight: 500;
+
padding: 0 0.75em 0.75em 0.75em;
+
text-align: left;
+
}
+
 
+
table thead {
+
border-bottom: solid 2px rgba(144, 144, 144, 0.25);
+
}
+
 
+
table tfoot {
+
border-top: solid 2px rgba(144, 144, 144, 0.25);
+
}
+
 
+
table.alt {
+
border-collapse: separate;
+
}
+
 
+
table.alt tbody tr td {
+
border: solid 1px rgba(144, 144, 144, 0.25);
+
border-left-width: 0;
+
border-top-width: 0;
+
}
+
 
+
table.alt tbody tr td:first-child {
+
border-left-width: 1px;
+
}
+
 
+
table.alt tbody tr:first-child td {
+
border-top-width: 1px;
+
}
+
 
+
table.alt thead {
+
border-bottom: 0;
+
}
+
 
+
table.alt tfoot {
+
border-top: 0;
+
}
+
 
+
/* Button */
+
 
+
input[type="submit"],
+
input[type="reset"],
+
input[type="button"],
+
.button {
+
-moz-appearance: none;
+
-webkit-appearance: none;
+
-o-appearance: none;
+
-ms-appearance: none;
+
appearance: none;
+
-moz-transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
+
-webkit-transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
+
-o-transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
+
-ms-transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
+
transition: background-color 0.2s ease-in-out, color 0.2s ease-in-out;
+
background-color: #192024;
+
border-radius: 6px;
+
border: 0;
+
color: #ffffff !important;
+
cursor: pointer;
+
display: inline-block;
+
font-weight: 300;
+
text-transform: uppercase;
+
letter-spacing: 0.125em;
+
font-size: 0.9em;
+
height: 2.85em;
+
line-height: 2.85em;
+
padding: 0 1.75em;
+
text-align: center;
+
text-decoration: none;
+
white-space: nowrap;
+
text-indent: 0.25em;
+
}
+
 
+
input[type="submit"]:hover,
+
input[type="reset"]:hover,
+
input[type="button"]:hover,
+
.button:hover {
+
background-color: #232d33;
+
}
+
 
+
input[type="submit"]:active,
+
input[type="reset"]:active,
+
input[type="button"]:active,
+
.button:active {
+
background-color: #0f1315;
+
}
+
 
+
input[type="submit"].icon,
+
input[type="reset"].icon,
+
input[type="button"].icon,
+
.button.icon {
+
padding-left: 1.35em;
+
}
+
 
+
input[type="submit"].icon:before,
+
input[type="reset"].icon:before,
+
input[type="button"].icon:before,
+
.button.icon:before {
+
margin-right: 0.5em;
+
}
+
 
+
input[type="submit"].fit,
+
input[type="reset"].fit,
+
input[type="button"].fit,
+
.button.fit {
+
display: block;
+
margin: 0 0 1em 0;
+
width: 100%;
+
}
+
 
+
input[type="submit"].small,
+
input[type="reset"].small,
+
input[type="button"].small,
+
.button.small {
+
font-size: 0.7em;
+
}
+
 
+
input[type="submit"].big,
+
input[type="reset"].big,
+
input[type="button"].big,
+
.button.big {
+
font-size: 1.2em;
+
height: 2.65em;
+
line-height: 2.65em;
+
}
+
 
+
input[type="submit"].alt,
+
input[type="reset"].alt,
+
input[type="button"].alt,
+
.button.alt {
+
background-color: transparent;
+
box-shadow: inset 0 0 0 1px rgba(144, 144, 144, 0.25);
+
color: #666f77 !important;
+
}
+
 
+
input[type="submit"].alt:hover,
+
input[type="reset"].alt:hover,
+
input[type="button"].alt:hover,
+
.button.alt:hover {
+
background-color: rgba(144, 144, 144, 0.075);
+
}
+
 
+
input[type="submit"].alt:active,
+
input[type="reset"].alt:active,
+
input[type="button"].alt:active,
+
.button.alt:active {
+
background-color: rgba(144, 144, 144, 0.2);
+
}
+
 
+
input[type="submit"].alt.icon:before,
+
input[type="reset"].alt.icon:before,
+
input[type="button"].alt.icon:before,
+
.button.alt.icon:before {
+
color: #bbb;
+
}
+
 
+
input[type="submit"].special,
+
input[type="reset"].special,
+
input[type="button"].special,
+
.button.special {
+
background-color: #629DD1;
+
color: #ffffff !important;
+
}
+
 
+
input[type="submit"].special:hover,
+
input[type="reset"].special:hover,
+
input[type="button"].special:hover,
+
.button.special:hover {
+
background-color: #76a9d7;
+
}
+
 
+
input[type="submit"].special:active,
+
input[type="reset"].special:active,
+
input[type="button"].special:active,
+
.button.special:active {
+
background-color: #4e91cb;
+
}
+
 
+
input[type="submit"].disabled, input[type="submit"]:disabled,
+
input[type="reset"].disabled,
+
input[type="reset"]:disabled,
+
input[type="button"].disabled,
+
input[type="button"]:disabled,
+
.button.disabled,
+
.button:disabled {
+
background-color: #555f66 !important;
+
box-shadow: inset 0 -0.15em 0 0 rgba(0, 0, 0, 0.15);
+
color: #fff !important;
+
cursor: default;
+
opacity: 0.25;
+
}
+
 
+
/* Wrapper */
+
 
+
.wrapper {
+
padding: 6em 0 4em 0;
+
}
+
 
+
.wrapper.style1 {
+
background: #fff;
+
color: #555f66;
+
}
+
 
+
.wrapper.style2 {
+
background: #192024;
+
color: #c5c7c8;
+
}
+
 
+
.wrapper.style2 h2, .wrapper.style2 h3, .wrapper.style2 h4, .wrapper.style2 h5, .wrapper.style2 h6 {
+
color: #ffffff;
+
}
+
 
+
.wrapper.style2 header p {
+
color: #a3a5a7;
+
}
+
 
+
.wrapper.style2 .button.alt {
+
box-shadow: inset 0 0 0 1px rgba(144, 144, 144, 0.75);
+
color: #ffffff !important;
+
}
+
 
+
.wrapper.style2 .button.alt:hover {
+
background-color: rgba(144, 144, 144, 0.275);
+
}
+
 
+
.wrapper.style2 .button.alt:active {
+
background-color: rgba(144, 144, 144, 0.4);
+
}
+
 
+
.wrapper.style2 .button.alt.icon:before {
+
color: #a3a5a7;
+
}
+
 
+
/* Header */
+
 
+
#skel-layers-wrapper {
+
padding-top: 3em;
+
}
+
 
+
#header {
+
background: #fff;
+
color: #555f66;
+
cursor: default;
+
height: 3em;
+
left: 0;
+
line-height: 3em;
+
position: fixed;
+
top: 0;
+
width: 100%;
+
z-index: 10000;
+
box-shadow: 0 0 0.1em 0 rgba(0, 0, 0, 0.25);
+
}
+
 
+
#header h1 {
+
color: #666f77;
+
font-weight: 500;
+
height: inherit;
+
left: 1.25em;
+
line-height: inherit;
+
margin: 0;
+
padding: 0;
+
position: absolute;
+
top: 0;
+
}
+
 
+
#header h1 a {
+
font-size: 1.25em;
+
}
+
 
+
#header nav {
+
  height: inherit;
+
  line-height: inherit;
+
  right: 1.25em;
+
  top: 0;
+
  vertical-align: middle;
+
  margin-left: 25%;
+
  width: 5%;
+
  margin: 0 auto;
+
}
+
 
+
#header nav > ul {
+
list-style: none;
+
margin: 0;
+
padding-left: 0;
+
}
+
 
+
#header nav > ul > li {
+
border-radius: 6px;
+
display: inline-block;
+
margin-left: 2em;
+
padding-left: 0;
+
}
+
 
+
#header nav > ul > li a {
+
-moz-transition: color 0.2s ease-in-out;
+
-webkit-transition: color 0.2s ease-in-out;
+
-o-transition: color 0.2s ease-in-out;
+
-ms-transition: color 0.2s ease-in-out;
+
transition: color 0.2s ease-in-out;
+
color: #555f66;
+
display: inline-block;
+
text-decoration: none;
+
}
+
 
+
#header nav > ul > li a:hover {
+
color: #629DD1;
+
}
+
 
+
#header nav > ul > li:first-child {
+
margin-left: 0;
+
}
+
 
+
#header nav > ul > li .button {
+
height: 2.25em;
+
line-height: 2.25em;
+
margin-bottom: 0;
+
padding: 0 1em;
+
position: relative;
+
top: -0.075em;
+
vertical-align: middle;
+
}
+
 
+
#header .container {
+
position: relative;
+
}
+
 
+
#header .container h1 {
+
left: 0;
+
}
+
 
+
#header .container nav {
+
right: 0;
+
}
+
 
+
/* Banner */
+
 
+
#banner {
+
background-color: #666666;
+
background-image: url("../images/banner.jpg");
+
background-size: cover;
+
background-position: center center;
+
background-repeat: no-repeat;
+
color: #d8d8d8;
+
padding: 14em 0;
+
text-align: center;
+
position: relative;
+
}
+
 
+
#banner:before {
+
content: '';
+
position: absolute;
+
left: 0;
+
top: 0;
+
width: 100%;
+
height: 100%;
+
background: rgba(64, 72, 80, 0.25);
+
}
+
 
+
#banner .inner {
+
position: relative;
+
z-index: 1;
+
}
+
 
+
#banner .inner :last-child {
+
margin-bottom: 0;
+
}
+
 
+
#banner h2, #banner h3, #banner h4, #banner h5, #banner h6 {
+
color: #ffffff;
+
}
+
 
+
#banner .button.alt {
+
box-shadow: inset 0 0 0 1px rgba(144, 144, 144, 0.75);
+
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Latest revision as of 17:50, 8 November 2015

IGEM Team:GSU


P4= Protein Products Pichia Plants


Our Project

Cannabinoids and opiates are widely used classes of pharmaceuticals; unfortunately, these drugs have strong psychoactive effects or can be addictive. Our project consists of two ideas, both revolving around utilizing bioengineered microorganisms to create non-psychoactive cannabinoids and non-addictive analgesics. To achieve this we developed two projects: (1) Manufacturing a protein expression system to produce CBDA synthase in tobacco plants using agrobacterium, (2) Engineering the pGAPα vector system to express the mambalgin in Pichia Pastoris as a continuation of the 2014 GSU iGEM project. Simultaneously, we developed a proof of concept using horseradish peroxidase. By the end of this project we hope to have produced a synthetic biological system to manufacture pharmaceutical alternatives for patients that suffer from diseases such as epilepsy, cancer, or chronic severe pain.




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