Difference between revisions of "Team:Georgia State"

 
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<html>
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<html>
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<style type="text/css">
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.wrapper {
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padding: 6em 0 4em 0;
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}
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.wrapper.style1 {
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<head>
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background: #fff;
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    <meta charset="utf-8" />
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color: #555f66;
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    <title>IGEM Team:GSU</title>
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}
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    <meta name="description" content="" />
 +
    <meta name="keywords" content="" />
 +
    <meta name="author" content="nileforest">
 +
    <meta name="viewport" content="width=device-width, initial-scale=1.0, maximum-scale=1.0" />
 +
    <!--[if IE]><meta http-equiv='X-UA-Compatible' content='IE=edge,chrome=1'><![endif]-->
  
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.wrapper.style2 {
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    <!-- Favicone Icon -->
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background: #192024;
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    <link rel="shortcut icon" type="image/x-icon" href="img/favicon.ico">
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color: #c5c7c8;
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    <link rel="icon" type="image/png" href="img/favicon.png">
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}
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    <link rel="apple-touch-icon" href="img/favicon.png">
  
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.wrapper.style2 h2, .wrapper.style2 h3, .wrapper.style2 h4, .wrapper.style2 h5, .wrapper.style2 h6 {
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    <!-- CSS -->
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color: #ffffff;
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    <link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Georgia_State/CSS?action=raw&ctype=text/css" />
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}
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<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:Georgia_State/CSS1?action=raw&ctype=text/css" />
 +
</head>
  
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.wrapper.style2 header p {
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<body class="full-intro background--dark">
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color: #a3a5a7;
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}
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.wrapper.style2 .button.alt {
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    <!-- Preloader -->
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box-shadow: inset 0 0 0 1px rgba(144, 144, 144, 0.75);
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    <section id="preloader">
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color: #ffffff !important;
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        <div class="loader" id="loader">
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}
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            <div class="loader-img"></div>
 +
        </div>
 +
    </section>
 +
    <!-- End Preloader -->
  
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.wrapper.style2 .button.alt:hover {
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    <!-- Site Wraper -->
−
background-color: rgba(144, 144, 144, 0.275);
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    <div class="wrapper">
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}
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        <!-- HEADER -->
 +
        <header class="header">
 +
            <div class="container">
  
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.wrapper.style2 .button.alt:active {
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                <!-- logo -->
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background-color: rgba(144, 144, 144, 0.4);
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                <div class="logo">
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}
+
                    <a href="https://2015.igem.org/Team:Georgia_State">
 +
                        <img style="margin-top: -10px;" class="l-black" src="https://static.igem.org/mediawiki/2015/a/a8/LogoBlack_GSUiGem.png" />
 +
                    </a>
 +
                </div>
 +
                <!--End logo-->
  
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.wrapper.style2 .button.alt.icon:before {
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color: #a3a5a7;
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}
+
  
 +
<!-- Navigation Menu -->
 +
                <nav class="navigation singlepage-nav">
 +
                    <ul>
 +
                        <li>
 +
                            <a href="https://2015.igem.org/Team:Georgia_State/Team">Team</a>
 +
                        </li>
 +
 +
                        <li>
 +
                            <a href="https://2015.igem.org/Team:Georgia_State/Project">Project</a>
 +
<ul>
 +
<li>
 +
  <a href="https://2015.igem.org/Team:Georgia_State/Project#Thing1">Mambalgin</a>
 +
</li>
 +
<li>
 +
  <a href="https://2015.igem.org/Team:Georgia_State/Project#Thing2">CBDA Synthase</a>
  
 +
</li>
 +
<li>
 +
  <a href="https://2015.igem.org/Team:Georgia_State/Parts">Parts Submitted</a>
  
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/* Banner */
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</li>
 +
<li>
 +
  <a href="https://2015.igem.org/Team:Georgia_State/Description">Part Improvement</a>
  
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#banner {
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</li>
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background-color: #666666;
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</ul>
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background-image: url("../images/banner.jpg");
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<li> <a href="https://2015.igem.org/Team:Georgia_State/Notebook">Notebook</a>
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background-size: cover;
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</li>
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background-position: center center;
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−
background-repeat: no-repeat;
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color: #d8d8d8;
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padding: 14em 0;
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−
text-align: center;
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−
position: relative;
+
−
}
+
  
−
#banner:before {
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<li> <a href="https://2015.igem.org/Team:Georgia_State/Practices">Practices</a>
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content: '';
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<ul>
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position: absolute;
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<li>
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left: 0;
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<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing1">Atlanta Science Festival</a>
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top: 0;
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</li>
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width: 100%;
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height: 100%;
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<li>
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background: rgba(64, 72, 80, 0.25);
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<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing2">Six Flags Education Day</a>
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}
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</li>
 +
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing3">Legislative Outreach</a>
 +
</li>
 +
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Practices#Thing4">Atlanta Botanical Gardens</a>
 +
</li>
 +
</ul>
 +
<li> <a href="https://2015.igem.org/Team:Georgia_State/Collaborations">Collaborations</a>
 +
<ul>
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Collaborations#ASF">Atlanta Science Festival</a>
 +
</li>
 +
 +
<li>
 +
<a href="https://2015.igem.org/Team:Georgia_State/Collaborations#Thing2">Project JBroth</a>
 +
</li>
 +
</ul>
  
−
#banner .inner {
+
<li> <a href="https://2015.igem.org/Team:Georgia_State/Safety">Safety</a>
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position: relative;
+
                        </li>
−
z-index: 1;
+
<li> <a href="https://2015.igem.org/Team:Georgia_State/Attributions">Attributions</a>
−
}
+
</li> 
 +
                        <li> <a href="https://2015.igem.org/Team:Georgia_State/ImageGallery">Images</a>
 +
                        </li>
 +
                   
 +
                     
 +
                        <li>
 +
                            <a href="https://2015.igem.org/Team:Georgia_State#contact-info">Contact</a>
 +
                        </li>
  
−
#banner .inner :last-child {
+
                    </ul>
−
margin-bottom: 0;
+
                </nav>
−
}
+
<!--End Navigation Menu -->
  
−
#banner h2, #banner h3, #banner h4, #banner h5, #banner h6 {
 
−
color: #ffffff;
 
−
}
 
  
−
#banner .button.alt {
 
−
box-shadow: inset 0 0 0 1px rgba(144, 144, 144, 0.75);
 
−
color: #ffffff !important;
 
−
}
 
  
−
#banner .button.alt:hover {
+
            </div>
−
background-color: rgba(144, 144, 144, 0.275);
+
        </header>
−
}
+
        <!-- END HEADER -->
  
−
#banner .button.alt:active {
+
<!-- Intro Section -->
−
background-color: rgba(144, 144, 144, 0.4);
+
        <section id="intro" class="full-screen-intro bg-img29">
−
}
+
            <div class="media-wapper bg-img parallax parallax-section1 overlay-dark" style="top: 250px; background-image: url(https://static.igem.org/mediawiki/2015/9/9a/GSUiGEM_AtlantaSkyline.jpg);">
 +
                <canvas id="pollyfill-canvas"></canvas>
 +
            </div>
 +
            <div class="full-cap-wraper">
 +
                <div class="content-caption light-color">
 +
                    <div class="container">
 +
                        <br>
 +
                      <h2 class="h1">P<sup>4</sup>= Protein Products Pichia Plants</h2>
 +
                        <br>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
        </section>
 +
        <div class="clearfix"></div>
 +
        <!-- End Intro Section -->
  
−
#banner .button.alt.icon:before {
+
<!--About Section-->
−
color: #c1c1c1;
+
        <section id="about" class="wow fadeIn ptb ptb-sm-80" style="background-color: #0387A6;">
−
}
+
            <div class="container">
 +
                <div class="row text-center">
 +
                    <div class="col-md-12">
 +
                        <h3 class="h4" style="text-align: center;color: white;">Our Project</h3>
 +
                        <div class="spacer-15"></div>
 +
                        <p class="lead"  style="color: white;" ><b> Cannabinoids and opiates are widely used classes of pharmaceuticals; unfortunately, these drugs have strong psychoactive effects or can be addictive. Our project consists of two ideas, both revolving around utilizing bioengineered microorganisms to create non-psychoactive cannabinoids and non-addictive analgesics. To achieve this we developed two projects: (1) Manufacturing a protein expression system to produce CBDA synthase in tobacco plants using agrobacterium, (2) Engineering the pGAPα vector system to express the mambalgin in Pichia Pastoris as a continuation of the 2014 GSU iGEM project. Simultaneously, we developed a proof of concept using horseradish peroxidase. By the end of this project we hope to have produced a synthetic biological system to manufacture pharmaceutical alternatives for patients that suffer from diseases such as epilepsy, cancer, or chronic severe pain.</p>
 +
                    </div>
 +
                </div>
 +
                   
 +
            </div>
 +
        </section>
 +
        <!-- End About Section-->
 +
        <hr />
  
−
#banner .button {
+
       
−
min-width: 12em;
+
       
−
}
+
        <hr />
 +
 +
        <hr />
  
−
#banner h2 {
+
        <!-- Client Logos Section -->
−
font-size: 3.5em;
+
        <section id="client-logos" class="wow fadeIn ptb ptb-sm-80">
−
line-height: 1em;
+
            <div class="container text-center">
−
margin: 0 0 0.5em 0;
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                <h3>Sponsors </h3>
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padding: 0;
+
            </div>
−
}
+
            <div class="spacer-60"></div>
 +
            <div class="container">
 +
                <div class="owl-carousel client-carousel nf-carousel-theme ">
 +
                    <div class="item">
 +
                        <div class="client-logo">
 +
                            <img src="https://static.igem.org/mediawiki/2015/5/56/GSUiGEM_STEM_logo.PNG" alt="" />
 +
                        </div>
 +
                    </div>
 +
                    <div class="item">
 +
                        <div class="client-logo">
 +
                            <img src="https://static.igem.org/mediawiki/2015/8/8d/GSUiGEM_biologylogo.png" alt="" />
 +
                        </div>
 +
                    </div>
 +
                    <div class="item">
 +
                        <div class="client-logo">
 +
                            <img src="https://static.igem.org/mediawiki/2015/b/ba/Idt_logo2015.jpg" alt="" />
 +
                        </div>
 +
                    </div>
 +
                    <div class="item">
 +
                        <div class="client-logo">
 +
                            <img src="https://static.igem.org/mediawiki/2015/0/0d/EmoryLogoCSE_GSUiGEM.JPG" alt="" />
 +
                        </div>
 +
                    </div>
 +
                   
 +
                </div>
 +
            </div>
 +
        </section>
 +
        <!-- End Client Logos Section -->
  
−
#banner p {
+
<!-- Social Section -->
−
font-size: 1.5em;
+
        <section id="social" class="gray-bg ptb ptb-sm-80">
−
margin-bottom: 2em;
+
            <div class="container text-center">
−
}
+
                <div class="row">
 +
                    <div class="col-md-8 col-md-offset-2">
 +
                        <h3>Connect With Us</h3>
 +
                    </div>
 +
                </div>
 +
                <div class="spacer-60"></div>
 +
                <div class="row">
 +
                    <div class="col-md-8 col-md-offset-2">
 +
                        <div class="row">
 +
                            <div class="col-md-6 col-sm-4 col-xs-6">
 +
                                <div class="page-icon-top">
 +
    <a href="https://twitter.com/GSUiGEM">
 +
                     
 +
<img src="https://static.igem.org/mediawiki/2015/5/5f/GSUiGEM-2015Twitter.png">
 +
                        </div>
 +
                              <h6><a class="Link" target="_blank" href="https://twitter.com/GSUiGEM">Twitter</a></h6>
 +
                                </div>
 +
                               
 +
                         
 +
                            <div class="col-md-6 col-sm-4 col-xs-6">
 +
                                <div class="page-icon-top">
 +
<a href="https://www.facebook.com/GSUiGEM">
 +
<img src="https://static.igem.org/mediawiki/2015/9/95/GSUiGEM-fb.png">
 +
                                </div>
 +
                                <h6><a class="Link" target="_blank" href="https://www.facebook.com/GSUiGEM">Facebook</a></h6>
 +
                            </div>
 +
                           
 +
                        </div>
 +
    </div>
 +
                </div>
 +
            </div>
 +
        </section>
 +
        <!-- End Social Section -->
  
−
#banner p a {
+
       
−
color: #ffffff;
+
−
text-decoration: none;
+
−
}
+
  
−
/* Footer */
+
        <hr />
  
−
#footer {
+
        <section id="contact-info" class="ptb ptb-sm-80">
−
background: #f8f8f8;
+
            <div class="container">
−
padding: 4em 0 6em 0;
+
                <div class="row">
−
}
+
                    <div class="col-md-4">
 +
                        <div class="contact-box-left">
 +
                            <div class="contact-icon-left">
 +
<img src="https://static.igem.org/mediawiki/2015/0/04/GSUiGEM-Location.png">
 +
</div>
 +
                            <h6>Address</h6>
 +
                            <p>
 +
                                iGEM Lab
 +
                                <br>
 +
                                24 Peachtree Center Ave SE,<br> Kell Hall 439<br>
 +
                                Atlanta, Georgia 30303
 +
                            </p>
  
−
#footer a {
+
                        </div>
−
color: inherit;
+
                    </div>
−
}
+
  
−
#footer .copyright {
+
                    <div class="col-md-4">
−
color: #bbb;
+
                        <div class="contact-box-left">
−
font-size: 0.9em;
+
                            <div class="contact-icon-left">
−
margin: 2em 0 0 0;
+
<img src="https://static.igem.org/mediawiki/2015/9/93/GSUiGEM-phone.png">
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padding: 0;
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</div>
−
text-align: center;
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                            <h6>Call Us</h6>
−
}
+
                            <p>
 +
                                <span>We're all millennials. We don't like talking on the phone. <a href="https://twitter.com/GSUiGEM">Send us a Tweet!</a></span><br />
 +
                            </p>
 +
                        </div>
 +
                    </div>
  
−
#footer .copyright li {
+
                    <div class="col-md-4">
−
border-left: solid 1px rgba(144, 144, 144, 0.25);
+
                        <div class="contact-box-left">
−
display: inline-block;
+
                            <div class="contact-icon-left">
−
list-style: none;
+
<img src="https://static.igem.org/mediawiki/2015/d/d2/GSUiGEM-email.png">
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margin-left: 1.5em;
+
</div>
−
padding-left: 1.5em;
+
                            <h6>Email</h6>
−
}
+
                            <p>
 +
                                <a href="mailto:mbrewer@gsu.edu">mbrewer@gsu.edu</a>
 +
                            </p>
 +
                        </div>
 +
                    </div>
  
−
#footer .copyright li:first-child {
+
                </div>
−
border-left: 0;
+
            </div>
−
margin-left: 0;
+
        </section>
−
padding-left: 0;
+
−
}
+
        <!-- END CONTENT ---------------------------------------------------------------------------->
  
−
#footer .icons a {
+
        <!-- FOOTER -->
−
color: #bbb;
+
        <footer class="footer pt-80">
−
}
+
  
−
            #content{
+
            <!-- Copyright Bar -->
−
                  width: 100%
+
            <section class="copyright pb-60">
−
              }
+
                <div class="container">
−
</style>
+
                    <p class="">
−
<head>
+
                        &copy; 2015 <a><b>GSU IGEM</b></a>. All Rights Reserved.
−
<title>SUPER COOLNESS</title>
+
                        <br />
−
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
+
                     
−
<meta name="description" content="" />
+
                    </p>
−
<meta name="keywords" content="" />
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                </div>
−
<!--[if lte IE 8]><script src="js/html5shiv.js"></script><![endif]-->
+
            </section>
−
<script src="js/jquery.min.js"></script>
+
            <!-- End Copyright Bar -->
−
<script src="js/skel.min.js"></script>
+
−
<script src="js/skel-layers.min.js"></script>
+
−
<script src="js/init.js"></script>
+
−
<noscript>
+
−
<link rel="stylesheet" href="css/skel.css" />
+
−
<link rel="stylesheet" href="css/style.css" />
+
−
<link rel="stylesheet" href="css/style-xlarge.css" />
+
−
</noscript>
+
−
</head>
+
−
<body id="top">
+
  
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Latest revision as of 17:50, 8 November 2015

IGEM Team:GSU


P4= Protein Products Pichia Plants


Our Project

Cannabinoids and opiates are widely used classes of pharmaceuticals; unfortunately, these drugs have strong psychoactive effects or can be addictive. Our project consists of two ideas, both revolving around utilizing bioengineered microorganisms to create non-psychoactive cannabinoids and non-addictive analgesics. To achieve this we developed two projects: (1) Manufacturing a protein expression system to produce CBDA synthase in tobacco plants using agrobacterium, (2) Engineering the pGAPα vector system to express the mambalgin in Pichia Pastoris as a continuation of the 2014 GSU iGEM project. Simultaneously, we developed a proof of concept using horseradish peroxidase. By the end of this project we hope to have produced a synthetic biological system to manufacture pharmaceutical alternatives for patients that suffer from diseases such as epilepsy, cancer, or chronic severe pain.

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24 Peachtree Center Ave SE,
Kell Hall 439
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