Difference between revisions of "Team:Bielefeld-CeBiTec/Collaborations"

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          <h2>Collaboration with iGEM team from Freiburg</h2>
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<p> Our team from Bielefeld sent a plasmid based on BBa_I746909 that has a translation enhancing sequence (5’-UTR), and team Freiburg sent a plasmid containing turboYFP, a His and a Halo Tag. We would like to compare if these parts work in different cell free proteins synthesis environments. </p>
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<h2>Collaboration with team<a href="https://2015.igem.org/Team:Dundee/Practices" target="_blank"> Dundee 2015</a>  </h2>
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<figcaption>Team Dundee 2015</figcaption>
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<p>Some aspects of our project were similar to team Dundee. They built a chromium biosensor based on the same system we used, the chromate inducible operon of <i>Ochrobactrum tritici</i> 5bvl1. Therefore, we decided to cooperate and exchanged ideas, plasmid maps and sequences. During our collaboration, we realized that they were comparing the original sequence of the repressor to one with the first 15 codons optimized, while our system is based on a complete codon optimized repressor. So we exchanged samples to test in each other’s systems and look for differences. We were able to <a href=" https://2015.igem.org/Team:Bielefeld-CeBiTec/Results/HeavyMetals " target="_blank"> measure</a> a combination of our promoter and their repressor in CFPS.</p>
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          <h2>Collaboration with team<a href=" https://2015.igem.org/Team:Dundee/Practices "_blank"> Dundee 2015</a>  </h2>
 
<p><img style="float: left; margin-right: 20px" src="https://static.igem.org/mediawiki/2015/f/fd/Bielefeld-CeBiTec_Collaboration_iGEM_Dundee_2015.jpeg"width="200px" />Some aspects of our project were similar to team Dundee. They build a chromium biosensor based on the same system we used, the chromate inducible operon of Ochrobactrum tritici 5bvl1. Therefore we decided to cooperate and exchanged ideas plasmid maps and sequences. Thereby we realized that they were comparing the original sequence to one with the first 15 codons optimized. While our system bases on a complete codon optimized repressor. So we exchanged samples to test in each other’s systems. And look for differences. We were able to <a href=" https://2015.igem.org/Team:Bielefeld-CeBiTec/Results/HeavyMetals "_blank"> measure</a> a combination of our promoter and their repressor in CFPS.</p>
 
  
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<h2>Collaboration with team <a href="https://2015.igem.org/Team:Santa_Clara" target="_blank">Santa Clara 2015</a></h2>
<h2>Collaboration with team <a href="https://2015.igem.org/Team:Santa_Clara"target="_blank">Santa Clara 2015</a></h2>
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<figcaption>Joseph Ayar</figcaption>
 
<figcaption>Joseph Ayar</figcaption>
 
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<p>As a part of our project, we provide a report about the dual use issue. An aspect of this report is the analysis of the legal situation in different countries, namely the applying laws in Germany, the European Union, and the United States of America. As an expert in the field of law, a member of team Santa Clara (Joseph Ayar, J.D. candidate from University of Santa Clara, School of Law) provided valuable insights in the applying laws and advisory boards in the USA. His analysis is marked as quotes in our <a href="https://static.igem.org/mediawiki/2015/b/be/Bielefeld-CeBiTec_Dual-Use_Report.pdf" target="_blank">report</a>.</p>
As a part of our project, we provide a report about the dual use issue. An aspect of this report is the analysis of the legal situation in different countries, namely the applying laws in Germany, the European Union, and the United States of America. As an expert in the field of law, a member of team Santa Clara (Joseph Ayar, J.D. candidate from University of Santa Clara, School of Law) provided valuable insights in the applying laws and advisory boards in the USA. His analysis is marked as quote in our <a href="https://2015.igem.org/team:Bielefeld-CeBiTec/Practices/DualUse"target="_blank">report</a>.</p>
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<h2>Collaboration with team <a href="https://2015.igem.org/Team:Exeter"target="_blank">Exeter 2015</a></h2>
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<h2>Collaboration with team <a href="https://2015.igem.org/Team:Exeter" target="_blank">Exeter 2015</a></h2>
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<img src="https://static.igem.org/mediawiki/2015/f/f4/Bielefeld-CeBiTec_Collaboration-Exeter.jpeg"  width="120px">
 
<img src="https://static.igem.org/mediawiki/2015/f/f4/Bielefeld-CeBiTec_Collaboration-Exeter.jpeg"  width="120px">
 
<figcaption>Team Exeter 2015</figcaption>
 
<figcaption>Team Exeter 2015</figcaption>
 
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<p>Similar to our project, team Exeter is working with a cell extract to translate proteins. They designed a synthetic toehold switch to detect specific RNA sequences. They aim to use that switch in a cell extract for further use in diagnostics. In terms of collaboration, we communicated via Skype and email. To help them to determine further applications of their project, we calculated the price for a single reaction of our crude cell extract in the size of their reaction and provided a table of contents. </p>
 
<p>Similar to our project, team Exeter is working with a cell extract to translate proteins. They designed a synthetic toehold switch to detect specific RNA sequences. They aim to use that switch in a cell extract for further use in diagnostics. In terms of collaboration, we communicated via Skype and email. To help them to determine further applications of their project, we calculated the price for a single reaction of our crude cell extract in the size of their reaction and provided a table of contents. </p>
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<p>We are thankful to team Exeter for reading our <a href="https://static.igem.org/mediawiki/2015/b/be/Bielefeld-CeBiTec_Dual-Use_Report.pdf" target="_blank">dual use report</a> and giving advice on language style.</p>
 
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<h2>Collaboration with iGEM team <a href=" https://2015.igem.org/Team:Freiburg/Collaborations " target="_blank"> Freiburg 2015</a></h2>
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<figure style="float: right; margin-left: 20px"><img src="https://static.igem.org/mediawiki/2015/5/58/Bielefeld-CeBiTec_Collaboration_iGEM_Freiburg_2015.jpg"width="150px">
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<figcaption>Team Freiburg 2015</figcaption></figure>
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<p>Both Freiburg and our team work on cell free protein synthesis as part of their project. Our team sent a plamid based on <a href="https://2015.igem.org/Team:Bielefeld-CeBiTec/Parts#BBa_I746909" target="_blank">BBa_I746909</a>, containing a translation enhancing sequence (5'-UTR) to team Freiburg. In return, they provided a plasmid containing turboYFP, a his-tag and a halo-tag (Promega). These parts were tested for their usefulness in different cell-free protein synthesis environments.</p>
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<h2> Collaborations</h2>
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
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<h4> Which other teams can we work with? </h4>
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You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the <a hreef="https://2015.igem.org/Judging/Awards#Medals">iGEM team collaboration gold medal criterion</a>.
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In order to meet the gold medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
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Here are some suggestions for projects you could work on with other teams:
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<li> Improve the function of another team's BioBrick Part or Device</li>
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<li> Characterize another team's part </li>
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<li> Debug a construct </li>
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<li> Model or simulating another team's system </li>
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<li> Test another team's software</li>
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<li> Help build and test another team's hardware project</li>
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<li> Mentor a high-school team</lei>
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Latest revision as of 14:15, 9 November 2015

iGEM Bielefeld 2015


Collaborations

Because research is more fun together.

Collaboration with team Dundee 2015

Team Dundee 2015

Some aspects of our project were similar to team Dundee. They built a chromium biosensor based on the same system we used, the chromate inducible operon of Ochrobactrum tritici 5bvl1. Therefore, we decided to cooperate and exchanged ideas, plasmid maps and sequences. During our collaboration, we realized that they were comparing the original sequence of the repressor to one with the first 15 codons optimized, while our system is based on a complete codon optimized repressor. So we exchanged samples to test in each other’s systems and look for differences. We were able to measure a combination of our promoter and their repressor in CFPS.

Collaboration with team Santa Clara 2015

Joseph Ayar

As a part of our project, we provide a report about the dual use issue. An aspect of this report is the analysis of the legal situation in different countries, namely the applying laws in Germany, the European Union, and the United States of America. As an expert in the field of law, a member of team Santa Clara (Joseph Ayar, J.D. candidate from University of Santa Clara, School of Law) provided valuable insights in the applying laws and advisory boards in the USA. His analysis is marked as quotes in our report.

Collaboration with team Exeter 2015

Team Exeter 2015

Similar to our project, team Exeter is working with a cell extract to translate proteins. They designed a synthetic toehold switch to detect specific RNA sequences. They aim to use that switch in a cell extract for further use in diagnostics. In terms of collaboration, we communicated via Skype and email. To help them to determine further applications of their project, we calculated the price for a single reaction of our crude cell extract in the size of their reaction and provided a table of contents.

We are thankful to team Exeter for reading our dual use report and giving advice on language style.

Collaboration with iGEM team Freiburg 2015

Team Freiburg 2015

Both Freiburg and our team work on cell free protein synthesis as part of their project. Our team sent a plamid based on BBa_I746909, containing a translation enhancing sequence (5'-UTR) to team Freiburg. In return, they provided a plasmid containing turboYFP, a his-tag and a halo-tag (Promega). These parts were tested for their usefulness in different cell-free protein synthesis environments.