Difference between revisions of "Team:Paris Bettencourt/Notebook/VitaminA"
| Line 8: | Line 8: | ||
<h3>Procedure</h3> | <h3>Procedure</h3> | ||
<ul> | <ul> | ||
| − | <li>Liquid culture overnight in LB.</li> | + | <li>Liquid culture overnight in LB + Ampicillin.</li> |
| − | <li>Centrifuge the | + | <li>Made a glycerol stock and stored it in the -20 freezer (g15.35)</li> |
| + | <li>Centrifuge the tube for 1 minute with 11000 rpm.</li> | ||
<li>Follow the standard protocol of Thermo Scientific miniprep kit <font color="red">(is it? I followed Ihab's protocol here: https://docs.google.com/document/d/1QHBHcN8RY0c-oEY8X5kAqPrKYODTBm7K8eU_lY_yw-o/edit, we should compare them)</font></li> | <li>Follow the standard protocol of Thermo Scientific miniprep kit <font color="red">(is it? I followed Ihab's protocol here: https://docs.google.com/document/d/1QHBHcN8RY0c-oEY8X5kAqPrKYODTBm7K8eU_lY_yw-o/edit, we should compare them)</font></li> | ||
<li>Measure concentration with Nanodrop</li> | <li>Measure concentration with Nanodrop</li> | ||
| − | + | ||
</ul> | </ul> | ||
Revision as of 09:10, 23 July 2015
Jul. 14th
Goal
Extract the integrative plasmid HO-Poly-KanMX4-HO from the E.Coli provided by AddGene (Accession number #51662).Procedure
- Liquid culture overnight in LB + Ampicillin.
- Made a glycerol stock and stored it in the -20 freezer (g15.35)
- Centrifuge the tube for 1 minute with 11000 rpm.
- Follow the standard protocol of Thermo Scientific miniprep kit (is it? I followed Ihab's protocol here: https://docs.google.com/document/d/1QHBHcN8RY0c-oEY8X5kAqPrKYODTBm7K8eU_lY_yw-o/edit, we should compare them)
- Measure concentration with Nanodrop
Results
Final DNA concentrations of the 4 tubes of miniprep, measured with Nanodrop:- tube HO pl. 1 = 431.1 ng/uL
- tube HO pl. 2 = 313.6 ng/uL
- tube HO pl. 3 = 366.4 ng/uL
- tube HO pl. 4 = 261.7 ng/uL
- Made the project, it is awesome!
Jul. 24th