Difference between revisions of "Team:Paris Bettencourt/Notebook/VitaminA"

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<h3>Procedure</h3>
 
<h3>Procedure</h3>
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<li>Liquid culture overnight in LB + Ampicillin.</li>
 
<li>Liquid culture overnight in LB + Ampicillin.</li>
 
<li>Made a glycerol stock and stored it in the -20 freezer (g15.35)</li>
 
<li>Made a glycerol stock and stored it in the -20 freezer (g15.35)</li>
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<li>Measure concentration with Nanodrop</li>
 
<li>Measure concentration with Nanodrop</li>
  
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</ul>
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<h3>Results</h3>
 
<h3>Results</h3>

Revision as of 10:29, 23 July 2015

Jul. 14th

Goal

Extract the integrative plasmid HO-Poly-KanMX4-HO from the E.Coli provided by AddGene (Accession number #51662).

Procedure

  1. Liquid culture overnight in LB + Ampicillin.
  2. Made a glycerol stock and stored it in the -20 freezer (g15.35)
  3. Centrifuge the tube for 1 minute with 11000 rpm.
  4. Follow the standard protocol of Thermo Scientific miniprep kit (is it? I followed Ihab's protocol here: https://docs.google.com/document/d/1QHBHcN8RY0c-oEY8X5kAqPrKYODTBm7K8eU_lY_yw-o/edit, we should compare them)
  5. Measure concentration with Nanodrop

Results

Final DNA concentrations of the 4 tubes of miniprep, measured with Nanodrop:
  • tube HO pl. 1 = 431.1 ng/uL
  • tube HO pl. 2 = 313.6 ng/uL
  • tube HO pl. 3 = 366.4 ng/uL
  • tube HO pl. 4 = 261.7 ng/uL


    • Jul. 24th
    • Made the project, it is awesome!