The Columbia University Department of Systems Biology is an interdisciplinary program based in New York City whose goal is to advance the integration of quantitative and experimental research methods in the biological and biomedical sciences. With a faculty of more than two dozen members and onsite research infrastructure for high-performance computing, next-generation sequencing, and high-throughput screening, we conduct research to understand the behavior of complex biological networks and how small changes in those networks produce the diversity of life around us. We also provide graduate education and postdoctoral training to increase understanding of systems biology and promote its use in biological research.