Difference between revisions of "Team:Michigan/Notebook/September"

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<h2 style="text-align: center">Septebmer</h2>
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<h2 style="text-align: center">September</h2>
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<p>
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Work plan:
 +
<br><br>
 +
LacZ into Thrombin plasmid:
 +
<br>
 +
<ul>
 +
  <li>cloning not working probably because the insert is almost as big as backbone
 +
let’s transform LacZ again</li>
 +
  <li transform in vitro LacZ part again</li>
 +
  <li>plasmid is ready, digest with HindiIII and A</li>
 +
  <li>gel extract plasmid, measure dna concentration</li>
 +
</ul>
  
 +
<br>
 +
LacZ into Gliadin plasmid:
 +
<br>
 +
Pfldh plasmid with GFP:
 +
<br>measure fluorescence
 +
<br><br>
 +
Things to order:
 +
<br>
 +
<ul>
 +
  <li>aptamer and trigger for Gliadin</li>
 +
  <li> switch for Gliadin with GFP (check design folder)</li>
 +
  <li>PfLDH protein</li>
 +
  <li>aptamer and trigger for Pfldh</li>
 +
  <li>aptamer and trigger for Kanamycin</li>
 +
</ul>
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 +
<img src="http://www.facebook.com/l.php?u=http%3A%2F%2F2015.igem.org%2FFile%3ASwitchThrombin.png&h=IAQEgSM9w" alt="MostImportant" style="width:304px;height:228px;">
  
 
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Revision as of 03:48, 19 September 2015

September

Work plan:

LacZ into Thrombin plasmid:

  • cloning not working probably because the insert is almost as big as backbone let’s transform LacZ again
  • plasmid is ready, digest with HindiIII and A
  • gel extract plasmid, measure dna concentration

LacZ into Gliadin plasmid:
Pfldh plasmid with GFP:
measure fluorescence

Things to order:
  • aptamer and trigger for Gliadin
  • switch for Gliadin with GFP (check design folder)
  • PfLDH protein
  • aptamer and trigger for Pfldh
  • aptamer and trigger for Kanamycin
MostImportant