Team:Michigan/Notebook/September
September
Work plan:
LacZ into Thrombin plasmid:
- cloning not working probably because the insert is almost as big as backbone let’s transform LacZ again
- transform in vitro LacZ part again
- plasmid is ready, digest with HindiIII and A
- gel extract plasmid, measure dna concentration
LacZ into Gliadin plasmid:
Pfldh plasmid with GFP:
measure fluorescence
Things to order:
- aptamer and trigger for Gliadin
- switch for Gliadin with GFP (check design folder)
- PfLDH protein
- aptamer and trigger for Pfldh
- aptamer and trigger for Kanamycin
we wanted to replicate results from previous experiment and try to detect lower thrombin concentrations to prove the sensitivity of our system
1 = Negative control (switch 1.0 + trigger-aptamer No Thrombin)
2 = Positive control (switch 1.0 + trigger)
3= Switch 1.0 + trigger + aptamer + 25uM Thrombin
4= Switch 1.0 + trigger + aptamer + 5uM Thrombin
5= Switch 1.0 + trigger + aptamer + 2.5uM Thrombin
6= Switch 1.0 + trigger + aptamer + 1.0uM Thrombin
7= Switch 1.0 + trigger + aptamer + 0.5uM Thrombin
8= Switch 1.0 + trigger + aptamer + 0.1uM Thrombin
