Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/13 August 2015"

(Colony PCR for M1-9(T7) and M1SeqAB1)
(Colony PCR for M1-9(T7) and M1SeqAB1)
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=08/13/2015=
 
=08/13/2015=
  
==Colony PCR for M1-9(T7) and M1SeqAB1==
+
==Colony PCR for M1-9(T7) and M1SeqAB2==
  
 
Same protocol from  [https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/12_August_2015 yesterday]
 
Same protocol from  [https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/12_August_2015 yesterday]

Revision as of 17:53, 17 August 2015

iGEM UCLA




08/13/2015

Colony PCR for M1-9(T7) and M1SeqAB2

Same protocol from yesterday

Ran samples on 1.5%

2015-08-13 17hr 01min.jpg

All samples showed bands of expected size M1-9(T7) Colony 3 has a slight smaller band than the others

PCR amplification of M1-12

Q5 buffer 10 uL
dNTPs 1 uL
F-03 2.5 uL
G-03 2.5 uL
ddH2O 32.5 uL
Template 1 uL
Q5 0.5 uL


PCR0813.001.jpg