Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/13 August 2015"

(Colony PCR for M1-9(T7) and M1SeqAB1)
(Colony PCR for M1-9(T7) and M1SeqAB2)
 
Line 13: Line 13:
 
All samples showed bands of expected size
 
All samples showed bands of expected size
 
M1-9(T7) Colony 3 has a slight smaller band than the others
 
M1-9(T7) Colony 3 has a slight smaller band than the others
 +
M1-Seq1AB is actually M1-Seq2AB
  
 
==PCR amplification of M1-12==
 
==PCR amplification of M1-12==

Latest revision as of 17:53, 17 August 2015

iGEM UCLA




08/13/2015

Colony PCR for M1-9(T7) and M1SeqAB2

Same protocol from yesterday

Ran samples on 1.5%

2015-08-13 17hr 01min.jpg

All samples showed bands of expected size M1-9(T7) Colony 3 has a slight smaller band than the others M1-Seq1AB is actually M1-Seq2AB

PCR amplification of M1-12

Q5 buffer 10 uL
dNTPs 1 uL
F-03 2.5 uL
G-03 2.5 uL
ddH2O 32.5 uL
Template 1 uL
Q5 0.5 uL


PCR0813.001.jpg