Difference between revisions of "Team:UNIK Copenhagen/Workshop"

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<h1>Presentations</h1>
 
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<font size="4">Presenting at the ph.D. summerschool </font> <br>
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<font size="4">Human Exploration and Space Flight</font> <br>
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<font size="3">Copenhagen, Denmark - August 27th 2015 </font>  
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<font size="3">Kiruna, Sweden August 15th 2015 </font>  
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<font size="4">Kick-off introduction at University of Copenhagen</font> <br>
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<font size="3">Copenhagen, Denmark - August 21th 2015 </font>  
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<img src="https://static.igem.org/mediawiki/2015/1/12/UNIK_Copenhagen_Kirunapresentation.jpg" width=72%></div>
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<img src="https://static.igem.org/mediawiki/2015/b/bf/UNIK_Copenhagen_Spacemossground.jpg" width=40%></div>
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Every year before university commences new students at physics attend a kick-off week where they get to meet each other, hear about physics and university life and get accustomed to their new environment. SpaceMoss dropped by to tell them how awesome it is to study physics and tell them to keep their eyes open for opportunities like the iGEM competetion and cross-disciplinary work.
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<p>Outside the scope of the competition we will insert the DNA gene containing YFP and an antifreeze protein gene and a resveratrol gene. When this is transformed into the moss we should have an output of moss that is able to produce the antifreeze protein on its own, or produce resveratrol. The moss will also glow yellow due to the YFP when it is producing either of the components, which acts as a proof of concept. </p>
 
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<font size="4">Technical description</font>
 
  
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<p>We will make a stable and transient transformation of moss, Psychometrilla patens. We will make two linear gene constructs for stable transformation in moss. One construct is going to contain a gene encoding an antifreeze protein and the other construct will contain a stilbene synthase-gene (STS), making the final enzyme in the resveratrol pathway. Both constructs contain regions homologous to the moss genome, so they will integrate via homologous recombination in moss. The constructs furthermore contains the ZmUbi-promoter, resistance gene and YFP, to confirm transformation. We will also make a transient moss transformation with a vector containing the ZmUbi-promoter followed by YFP, to confirm the function of the promoter in moss. </p>
 
  
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<font size="4">Human Exploration and Space Flight</font> <br>
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<font size="3">Kiruna, Sweden - August 15th 2015 </font>
 
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SpaceMoss attended the course Human Exploration and Space Flight in Kiruna (Northern Sweden). The course is about the historical development of human spaceflight, an understanding of the requirements to be met in order to send humans into space now and in the future. During the course we discussed our idea with respected scientists and even an astronaut, launched rocket SpaceMoss and held a presentation for the course participants.<br>
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<img src="https://static.igem.org/mediawiki/2015/3/3e/UNIK_copenhagen_mossbasement.jpg" width=62%></div>
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Read more about the course <a href="http://www.irf.se/~carol/summer/">here</a>  
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Moss growing facility</div>
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<p>Due to time constraints, we will also transform tobacco and <i>E.coli</i>. We will transform tobacco with the STS-gene, using USER-cloning and Agrobacterium tumefaciens transformation, in order to quickly confirm the functionality of the gene (moss takes a long time to grow!) by measuring the presence of resveratrol with Liquid chromatography-mass spectrometry.
 
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Furthermore we will transform E. coli with the antifreeze gene which originate from the spruce budworm, to quickly see whether the gene has an effect on temperature sensitivity. We will perform experiments at low temperatures to measure the growth of the bacteria when exposed to cold. We will also make a his-tag version of the antrifreezeprotein, to confirm the presence of the protein by western blotting.</p>
 
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     <img src="https://static.igem.org/mediawiki/2015/b/b9/UNIK_copenhagen_mossinhand.jpg" height="512" width="341.25" />
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     <img src="https://static.igem.org/mediawiki/2015/1/12/UNIK_Copenhagen_Kirunapresentation.jpg" height="400" width="400" />
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     <p>One of our petri dishes with moss</p>
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     <p>Christina presenting</p>
 
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     <img class="middle-img" src="https://static.igem.org/mediawiki/2015/8/89/UNIK_copenhagen_mossfacility.jpg"/ height="512" width="341.25" />
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     <img class="middle-img" src="https://static.igem.org/mediawiki/2015/b/bf/UNIK_Copenhagen_Spacemossground.jpg"/ height="200" width="200" />
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    <p>Rocket SpaceMoss</p>
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    <img class="middle-img" src="https://static.igem.org/mediawiki/2015/d/da/UNIK_Copenhagen_Spacemosslaunch.jpg"/ height="200" width="200" />
 
     <p>Moss growing facility</p>
 
     <p>Moss growing facility</p>
 
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Revision as of 14:29, 19 August 2015


Presentations

Presenting at the ph.D. summerschool
Copenhagen, Denmark - August 27th 2015

Kick-off introduction at University of Copenhagen
Copenhagen, Denmark - August 21th 2015

Every year before university commences new students at physics attend a kick-off week where they get to meet each other, hear about physics and university life and get accustomed to their new environment. SpaceMoss dropped by to tell them how awesome it is to study physics and tell them to keep their eyes open for opportunities like the iGEM competetion and cross-disciplinary work.



Human Exploration and Space Flight
Kiruna, Sweden - August 15th 2015

SpaceMoss attended the course Human Exploration and Space Flight in Kiruna (Northern Sweden). The course is about the historical development of human spaceflight, an understanding of the requirements to be met in order to send humans into space now and in the future. During the course we discussed our idea with respected scientists and even an astronaut, launched rocket SpaceMoss and held a presentation for the course participants.
Read more about the course here



Christina presenting

Rocket SpaceMoss

Moss growing facility