Difference between revisions of "Team:UCLA/Notebook/Protein Cages/21 August 2015"
| Line 5: | Line 5: | ||
Expression Culture: | Expression Culture: | ||
| − | OD with 4 mL of starter culture: 0.009 | + | OD with 4 mL of starter culture: 0.009, 0.023* |
| − | OD with all of starter culture: 0.019 | + | OD with all of starter culture: 0.019, 0.049* |
Incubation with all of starter culture began at 1:25 pm. | Incubation with all of starter culture began at 1:25 pm. | ||
| − | OD after one hour: 0.022 | + | OD after one hour: 0.022, 0.073* |
| + | |||
| + | OD after two hours: 0.045, 0.112* | ||
Revision as of 22:44, 21 August 2015
Intro: Grew the starter culture overnight for ~16 hours. Refreshed into 300 mL expression culture. Induced with IPTG.
Expression Culture:
OD with 4 mL of starter culture: 0.009, 0.023*
OD with all of starter culture: 0.019, 0.049*
Incubation with all of starter culture began at 1:25 pm.
OD after one hour: 0.022, 0.073*
OD after two hours: 0.045, 0.112*