Difference between revisions of "Team:UCLA/Notebook/Protein Cages/21 August 2015"

Line 5: Line 5:
 
Expression Culture:
 
Expression Culture:
  
−
OD with 4 mL of starter culture: 0.009, 0.023*
+
OD with 4 mL of starter culture: 0.023
  
−
OD with all of starter culture: 0.019, 0.049*
+
OD with all of starter culture: 0.049
  
 
Incubation with all of starter culture began at 1:25 pm.
 
Incubation with all of starter culture began at 1:25 pm.
  
−
OD after one hour: 0.022, 0.073*
+
OD after one hour: 0.073
  
−
OD after two hours: 0.045, 0.112*
+
OD after two hours: 0.112
 +
 
 +
OD after 3.5 hours: 0.443

Revision as of 00:10, 22 August 2015

iGEM UCLA




Intro: Grew the starter culture overnight for ~16 hours. Refreshed into 300 mL expression culture. Induced with IPTG.

Expression Culture:

OD with 4 mL of starter culture: 0.023

OD with all of starter culture: 0.049

Incubation with all of starter culture began at 1:25 pm.

OD after one hour: 0.073

OD after two hours: 0.112

OD after 3.5 hours: 0.443