Difference between revisions of "Team:UCLA/Notebook/Materials/Quick Reference"
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==Wetspinning== | ==Wetspinning== | ||
| − | *15% w/v aqueous silk extruding at rate of 10uL/min, BD plastic 1mL syringe, into 90% v/v Isopropanol bath, comes out at a rate of '''7.3846 mm/s''' | + | *15% w/v aqueous silk extruding at rate of 10uL/min, BD plastic 1mL syringe, into 90% v/v Isopropanol bath, comes out at a rate of [https://2015.igem.org/Team:UCLA/Notebook/Materials/26_August_2015 '''7.3846 mm/s'''] |
Latest revision as of 08:04, 29 August 2015
Dialysis
- For a 10 mL sample, 10 mm of dialysis tubing is too long
- However, having that several mm of clearance at the top to seal with the clips is good.
- Dialysis changes for LiBr silk: 1hr, 4hr, "that evening," morning, night, morning
Wetspinning
- 15% w/v aqueous silk extruding at rate of 10uL/min, BD plastic 1mL syringe, into 90% v/v Isopropanol bath, comes out at a rate of 7.3846 mm/s