Difference between revisions of "Team:Nagahama/Experiments"
(→Agarose gel) |
(→LB medium (1L liquid)) |
||
| Line 4: | Line 4: | ||
<br><br> | <br><br> | ||
== Agarose gel == | == Agarose gel == | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
== LB medium (solid, 1L = 50 dishes) == | == LB medium (solid, 1L = 50 dishes) == | ||
Revision as of 06:24, 30 May 2015
Contents
Protocols
Agarose gel
LB medium (solid, 1L = 50 dishes)
- 15 g agar agar
- 10 g tryptone
- 10 g NaCl
- 5 g yeast extract
- Water
For selective medium, suplement with antibiotic as appropiate (kanamycin 50 µg/ mL and 100 µg/mL for chloramphenicol or ampicillin ).
Genome extraction
For bacterial genome extraction we used Easy DNA Kit, Invitrogen according to <a href="https://2014.igem.org/File:Easy-DNA_Kit.pdf" target="_blank">manufacturer's instructions</a>.
Plasmids extraction
For bacterial plasmid extraction we used GenElute Plasmid Miniprep Kit, Sigma Aldrich according to <a href="https://2014.igem.org/File:Miniprep.pdf" target="_blank">manufacturer's instructions</a>.
>
Experiments & Protocols
Describe the experiments, research and protocols you used in your iGEM project.
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project