Difference between revisions of "Team:Nagahama/Experiments"

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== '''Protocols''' ==
 
== '''Protocols''' ==
 
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== LB medium (100 mL liquid) ==
 
  
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== Agarose gel ==
 
  
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== LB medium (solid, 1L = 50 dishes) ==
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=== Agarose gel ===
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=== LB medium (100 mL liquid) ===
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===  Genome extraction ===
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<li>15 g agar agar</li>
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<li>10 g tryptone</li>
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<li>10 g NaCl</li>
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<li>5 g yeast extract</li>
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<li>Water</li>
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For selective medium, suplement with antibiotic as appropiate (kanamycin 50 µg/ mL and 100 µg/mL for chloramphenicol or ampicillin ).
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==  Genome extraction ==
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== Plasmids extraction ==
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=== Plasmids extraction ===
 
   
 
   
 
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Revision as of 06:26, 30 May 2015

Team Nagahama banner.jpg

Protocols




Agarose gel

LB medium (100 mL liquid)

Genome extraction



For bacterial genome extraction we used Easy DNA Kit, Invitrogen according to <a href="https://2014.igem.org/File:Easy-DNA_Kit.pdf" target="_blank">manufacturer's instructions</a>.




Plasmids extraction



For bacterial plasmid extraction we used GenElute Plasmid Miniprep Kit, Sigma Aldrich according to <a href="https://2014.igem.org/File:Miniprep.pdf" target="_blank">manufacturer's instructions</a>.





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Experiments & Protocols

Describe the experiments, research and protocols you used in your iGEM project.

What should this page contain?
  • Protocols
  • Experiments
  • Documentation of the development of your project

Inspiration