Difference between revisions of "Team:Nagahama/Experiments"

(Protocols)
(Agarose gel(100mL))
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2.Add 100mL H2O
 
2.Add 100mL H2O
  
3.Heat(till agarose melted)
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3.Heat(till agarose melted)*We used a microwave oven.
  
 
4.Pur agarose into a gel maker
 
4.Pur agarose into a gel maker

Revision as of 04:09, 31 May 2015

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Protocols

Our Lab's Protocols

Agarose gel(100mL)

Method of Making 0.7% Agarose gel

1.Measure 0.7g Agarose

2.Add 100mL H2O

3.Heat(till agarose melted)*We used a microwave oven.

4.Pur agarose into a gel maker

5.Set a comb

6.Wait till agarose curdles

7.Pull an comb

LB medium (100 mL liquid)

1.Measure 1g Tripton

2.Measure 0.5g Yeast Extract

3.Measure 1g Nacl

4.Add 100mL H2O

5.autoclave(121℃ 20min)

Genome extraction

Plasmids extraction

Experiments

Experiments & Protocols

Describe the experiments, research and protocols you used in your iGEM project.

What should this page contain?
  • Protocols
  • Experiments
  • Documentation of the development of your project

Inspiration