Difference between revisions of "Team:Marburg/Labbook/InterLab"
Line 314: | Line 314: | ||
− | <h1> | + | <h1>TEXT</h1> |
+ | <p> | ||
+ | <h2>Text</h2> | ||
+ | lalala<br> | ||
+ | <br> | ||
+ | |||
+ | <h1>15/04/22</h1> | ||
+ | <p> | ||
+ | <h2>Primer Design</h2> | ||
+ | Primer Design iILS1-9<br> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | <h1>15/04/26</h1> | ||
+ | <p> | ||
+ | <h2>Miniprep</h2> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | <h1>15/06/03</h1> | ||
+ | <p> | ||
+ | <h2>PCR</h2> | ||
+ | <div> | ||
+ | <table valign="top" border="1" cellpadding="0" cellspacing="0"> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <p> | ||
+ | PCR Mix | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | PCR 1 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | PCR 2 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | PCR 3 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | PCR 4 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | PCR 5 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | PCR 6 | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <p> | ||
+ | 0,5 uL | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Backbone 23108 (BB1) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GFP 3504 | ||
+ | </p> | ||
+ | <p> | ||
+ | (GFP1) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | BB 23109 | ||
+ | </p> | ||
+ | <p> | ||
+ | (BB2) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GFP 3504 | ||
+ | </p> | ||
+ | <p> | ||
+ | (GFP2) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | BB 111 | ||
+ | </p> | ||
+ | <p> | ||
+ | (BB3) | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | GFP 3504 | ||
+ | </p> | ||
+ | <p> | ||
+ | (GFP3) | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <p> | ||
+ | 1 uL | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 2 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 4 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 2 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 7 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 2 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 9 | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <p> | ||
+ | 1 uL | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 3 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 5 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 6 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 5 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 8 | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Primer ilLS 5 | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <p> | ||
+ | 31,5 uL | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | ddH2O | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | ddH2O | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | ddH2O | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | ddH2O | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | ddH2O | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | ddH2O | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <p> | ||
+ | 10 uL | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Puffer | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Puffer | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Puffer | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Puffer | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Puffer | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Puffer | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <p> | ||
+ | 4 uL | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | dNTPs | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | dNTPs | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | dNTPs | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | dNTPs | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | dNTPs | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | dNTPs | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <p> | ||
+ | 2 uL | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Polymerase | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Polymerase | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Polymerase | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Polymerase | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Polymerase | ||
+ | </p> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | Polymerase | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | <br> | ||
+ | <h2>Text</h2> | ||
+ | lalala<br> | ||
+ | <br> | ||
+ | |||
+ | <h1>15/09/02</h1> | ||
<p> | <p> | ||
<h2>Transformation</h2> | <h2>Transformation</h2> | ||
Line 327: | Line 671: | ||
<h2>CPEC</h2> | <h2>CPEC</h2> | ||
CPEC of construct 5 <br> | CPEC of construct 5 <br> | ||
+ | <br> | ||
<br> | <br> | ||
− | |||
− | |||
− | |||
<h1>TEXT</h1> | <h1>TEXT</h1> | ||
− | |||
<p> | <p> | ||
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Revision as of 12:51, 11 September 2015
TEXT
Text
lalala15/04/22
Primer Design
Primer Design iILS1-915/04/26
Miniprep
15/06/03
PCR
PCR Mix |
PCR 1 |
PCR 2 |
PCR 3 |
PCR 4 |
PCR 5 |
PCR 6 |
0,5 uL |
Backbone 23108 (BB1) |
GFP 3504 (GFP1) |
BB 23109 (BB2) |
GFP 3504 (GFP2) |
BB 111 (BB3) |
GFP 3504 (GFP3) |
1 uL |
Primer ilLS 2 |
Primer ilLS 4 |
Primer ilLS 2 |
Primer ilLS 7 |
Primer ilLS 2 |
Primer ilLS 9 |
1 uL |
Primer ilLS 3 |
Primer ilLS 5 |
Primer ilLS 6 |
Primer ilLS 5 |
Primer ilLS 8 |
Primer ilLS 5 |
31,5 uL |
ddH2O |
ddH2O |
ddH2O |
ddH2O |
ddH2O |
ddH2O |
10 uL |
Puffer |
Puffer |
Puffer |
Puffer |
Puffer |
Puffer |
4 uL |
dNTPs |
dNTPs |
dNTPs |
dNTPs |
dNTPs |
dNTPs |
2 uL |
Polymerase |
Polymerase |
Polymerase |
Polymerase |
Polymerase |
Polymerase |
Text
lalala15/09/02
Transformation
Trafo of constr. 10,4+11,4+12,4 in NEB TurboPCR
PCR of P30+31PCR Cleanup
PCR-Clean up of P30+31CPEC
CPEC of construct 5TEXT
Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet.
Duis autem vel eum iriure dolor in hendrerit in vulputate velit esse molestie consequat, vel illum dolore eu feugiat nulla facilisis at vero eros et accumsan et iusto odio dignissim qui blandit praesent luptatum zzril delenit augue duis dolore te feugait nulla facilisi. Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat.