Difference between revisions of "Team:FAU Erlangen/Tour23"
Guenter FAU (Talk | contribs) |
Guenter FAU (Talk | contribs) |
||
Line 7: | Line 7: | ||
Once upon a time, there was a Type III effector… | Once upon a time, there was a Type III effector… | ||
</h1> | </h1> | ||
− | < | + | |
− | TALEs (Transcription-Activator-Like Effectors) are a family of effector molecules first discovered in Xanthomonas. The first TAL effector gene AvrBs3 was described more than 25 years ago. It consists of two constant domains, one localized on the C-Terminus and N-Terminus each and a central domain consisting of 17 repeats of 34 amino acids with two hypervariable positions, called RVDs (Repeat-Variable Di-residues). (Bonas, Stall et al., 1989). The actual number of tandem repeats in TALEs is variable and varies in accordance to the target sequence (Marois et al., 2002). TAL effectors were found to be able to induce gene expression in plants by binding a specific sequence in the promoter region (Römer et al., 2007; Kay et al., 2007) though the exact mechanism by which they did so remained unknown until 2009, when a correlation between the DNA sequence necessary for gene regulation by AvrBs3 and the sequence of RVDs in the effector protein was discovered (Boch et al., 2009). By breaking the code of TAL specifity it was now possible to create new TAL effectors binding specific, purposefully targeted DNA sequences. | + | <span class="intro">TALEs (Transcription-Activator-Like Effectors)</span> are a family of effector molecules first discovered in Xanthomonas. The first TAL effector gene AvrBs3 was described more than 25 years ago. It consists of two constant domains, one localized on the C-Terminus and N-Terminus each and a central domain consisting of 17 repeats of 34 amino acids with two hypervariable positions, called RVDs (Repeat-Variable Di-residues). (Bonas, Stall et al., 1989). The actual number of tandem repeats in TALEs is variable and varies in accordance to the target sequence (Marois et al., 2002). TAL effectors were found to be able to induce gene expression in plants by binding a specific sequence in the promoter region (Römer et al., 2007; Kay et al., 2007) though the exact mechanism by which they did so remained unknown until 2009, when a correlation between the DNA sequence necessary for gene regulation by AvrBs3 and the sequence of RVDs in the effector protein was discovered (Boch et al., 2009). By breaking the code of TAL specifity it was now possible to create new TAL effectors binding specific, purposefully targeted DNA sequences. |
</p.intro> | </p.intro> | ||
By the way, they’re actually useful: | By the way, they’re actually useful: | ||
Line 27: | Line 27: | ||
</p> | </p> | ||
</html> | </html> | ||
− | {{ | + | {{FAU |
Revision as of 16:40, 14 September 2015
Adrian: Bilder