Team:FAU Erlangen/Tour34
Set backs
“Science never solves a problem without creating ten more” - George Bernard Shaw
Sequences
- We planned to work with three different loci, yet forgot to synthesize a selectionmarker within the sequence for the recombination.
- Due to misunderstanding the only working homologous insert for the can1 gene was not used.
rpd3
- the rpd3 clones sent for sequencing always contained mutations
Golden Gate
- we ordered a wrong enzyme with a way too high temperature optimum --> reaction had very little production
- accidentally digestion of our Golden Gate fragments over night with an enzyme with star activity
