Difference between revisions of "Team:NRP-UEA-Norwich/Parts"

Line 188: Line 188:
 
  <tr><td>P-Lac(R0010)_RBS(B0034)_PTB</td><td>pSB1C3</td><td>Phosphotransbutrylase expression construct</td><td>Catalyzes the conversion  butyryl to/from butyryl CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618013">BBa_K1618013</a></td></tr>
 
  <tr><td>P-Lac(R0010)_RBS(B0034)_PTB</td><td>pSB1C3</td><td>Phosphotransbutrylase expression construct</td><td>Catalyzes the conversion  butyryl to/from butyryl CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618013">BBa_K1618013</a></td></tr>
  
−
   <tr><td>P-Lac(R0010)_RBS(B0034)_Buk_PTB</td><td>pSB1C3</td><td>utyrate to butyryl CoA pathway</td><td> Catalyzes the conversion  butyryl to/from butyryl CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618020">BBa_K1618020</a></td></tr>
+
   <tr><td>P-Lac(R0010)_RBS(B0034)_Buk_PTB</td><td>pSB1C3</td><td>Butyrate to butyryl CoA pathway</td><td> Catalyzes the conversion  butyryl to/from butyryl CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618020">BBa_K1618020</a></td></tr>
  
 
  <tr><td>P-Lac(R0010)_RBS(B0034)_THL</td><td>pSB1C3</td><td>Thiolase expression construct</td><td>Catalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618014">BBa_K1618014</a></td></tr>
 
  <tr><td>P-Lac(R0010)_RBS(B0034)_THL</td><td>pSB1C3</td><td>Thiolase expression construct</td><td>Catalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618014">BBa_K1618014</a></td></tr>
Line 217: Line 217:
  
  
−
<tr><td>Plac-GlgX</td><td>pSB1C3</td><td>Debranching enzyme with IPTG inducible Promoter</td><td>Removes E. coli glycogen branches</td><td><a href="http://parts.igem.org/Part:BBa_K1618025">BBa_K1618025</a></td></tr>
+
<h2 class="title1">COMPOUND PARTS FOR ENGINEERING PLANT STARCH</h2>
 +
    <div class="space10"></div>
  
−
<tr><td>GlgX</td><td>pSB1C3</td><td>Debranching enzyme</td><td>&nbsp;</td> <td><a href="http://parts.igem.org/Part:BBa_K1618026">BBa_K1618026</a></td></tr>
 
  
−
<tr><td>Plac-GlgB</td><td>pSB1C3</td><td>Branching enzyme with IPTG inducible Promoter</td><td>Add E. coli glycogen branches</td><td><a href="http://parts.igem.org/Part:BBa_K1618022">BBa_K1618022</a></td></tr>
+
<table style="width:100%">
 +
<tr>
 +
    <th>Part Contents</th>
 +
    <th>Backbone</th>  
 +
  <th>Description</th>
 +
<th>Function</th>
 +
  <th>Part number</th>
 +
  </tr>
  
−
<tr><td>GlgB</td><td>pSB1C3</td><td>Branching Enzyme</td><td>&nbsp;</td> <td>BBa_K1618000</td></tr>
 
−
<tr><td>Plac-MAO</td><td>pSB1C3</td><td>Acylation candidate 1 with IPTG inducible Promoter</td><td>Acylates glycogen? (1)</td><td><a href="http://parts.igem.org/Part:BBa_K1618005">BBa_K1618005</a> </td></tr>
 
  
−
<tr><td>MAO</td><td>pSB1C3</td><td>Acylation Candidate 1</td><td>&nbsp;</td> <td>BBa_K1618001</td></tr>
+
−
<tr><td>Plac-Rv3030</td><td>pSB1C3</td><td>Acylation candidate 2 with IPTG inducible Promoter</td><td>Acylates glycogen? (2)</td><td><a href="http://parts.igem.org/Part:BBa_K1618007">BBa_K1618007</a></td></tr>
+
  
−
<tr><td>Rv3030</td><td>pSB1C3</td><td>Acylation candidate 2</td><td>&nbsp;</td><td>BBa_K1618002</td></tr>
 
−
<tr><td>Plac-Rv3034c</td><td>pSB1C3</td><td>Acylation candidate 3 with IPTG inducible Promoter</td><td>Acylates glycogen? (3)</td> <td><a href="http://parts.igem.org/Part:BBa_K1618008">BBa_K1618008</a></td></tr>
 
  
−
<tr><td>Rv3034c</td><td>pSB1C3</td><td>Acylation candidate 3</td><td>&nbsp;</td> <td><a href="http://parts.igem.org/Part:BBa_K1618003">BBa_K1618003</a></td></tr>
 
  
−
<tr><td>Plac-Rv3037c</td><td>pSB1C3</td><td>Acylation candidate 4 with IPTG inducible Promoter</td><td>Acylates glycogen? (4)</td> <td><a href="http://parts.igem.org/Part:BBa_K1618009">BBa_K1618009</a></td></tr>
 
  
−
 
−
<tr><td>Rv3037c</td><td>pSB1C3</td><td>Acylation candidate 4</td><td>&nbsp;</td> <td><a href="http://parts.igem.org/Part:BBa_K1618004">BBa_K1618004</a></td></tr>
 
−
 
−
 
−
<tr><td>Plac-GlgB-Rv3030</td><td>pSB1C3</td><td>Branching enzyme + Acylation candidate 2 with IPTG inducible Promoter</td><td>Makes acetylated branched glycogen? (2)</td><td><a href="http://parts.igem.org/Part:BBa_K1618023">BBa_K1618023</a></td></tr>
 
−
 
−
<tr><td>Plac-GlgB-Rv3034c</td><td>pSB1C3</td><td>Branching enzyme + Acylation candidate 3 with IPTG inducible Promoter</td><td>Makes acetylated branched glycogen? (3)</td><td><a href="http://parts.igem.org/Part:BBa_K1618024">BBa_K1618024</a></td></tr>
 
−
 
−
<tr><td>Plac-GlgB-Rv3037c</td><td>pSB1C3</td><td>Branching enzyme + Acylation candidate 4 with IPTG inducible Promoter</td><td>Makes acetylated branched glycogen? (4)</td><td><a href="http://parts.igem.org/Part:BBa_K1618027">BBa_K1618027</a></td></tr>
 
−
 
−
<tr><td>Plac-Buk</td><td>pSB1C3</td><td>Butyrate kinase with IPTG inducible Promoter</td><td>Reversible reaction that makes butyrate from butyryl phosphate</td><td><a href="http://parts.igem.org/Part:BBa_K1618012">BBa_K1618012</a></td></tr>
 
−
 
−
<tr><td>Plac-PTB</td><td>pSB1C3</td><td>Phosphotransbutrylase with IPTG inducible Promoter</td><td>Reversible reaction that makes butyryl-phosphate from butyryl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618013">BBa_K1618013</a></td></tr>
 
−
 
−
<tr><td>Plac-THL</td><td>pSB1C3</td><td>Thiolase with IPTG inducible Promoter</td><td>Makes Acetoacetyl-CoA from Acetyl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618014">BBa_K1618014</a></td></tr>
 
−
 
−
<tr><td>Plac-CRO</td><td>pSB1C3</td><td>Crotonase with IPTG inducible Promoter</td><td>Makes Crotonyl-CoA from 3-hydroxybutyryl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618015">BBa_K1618015</a></td></tr>
 
−
 
−
<tr><td>Plac-BHBD</td><td>pSB1C3</td><td>&#946;-hydroxybutyryl-CoA dehydrogenase with IPTG inducible Promoter</td><td>Makes 3-hydroxybutyryl-CoA from Acetoacetyl-CoA </td><td><a href="http://parts.igem.org/Part:BBa_K1618016">BBa_K1618016</a></td></tr>
 
−
 
−
<tr><td>Plac-BCD</td><td>pSB1C3</td><td>Butyryl-CoA dehydrogenase with IPTG inducible Promoter</td><td>Makes Butyryl-CoA from Crotonyl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618017">BBa_K1618017</a></td></tr>
 
−
 
−
<tr><td>Plac-EFTb</td><td>pSB1C3</td><td>Electron transfer protein &#946; with IPTG inducible Promoter</td><td>Makes butyrate from acetate part 5</td><td><a href="http://parts.igem.org/Part:BBa_K1618018">BBa_K1618018</a></td></tr>
 
−
 
−
<tr><td>Plac-EFTa</td><td>pSB1C3</td><td>Electron transfer protein &#945; with IPTG inducible Promoter</td><td>Makes butyrate from acetate part 6</td><td>BBa_K1618019</td></tr>
 
−
 
−
<tr><td>Plac-Buk-PTB</td><td>pSB1C3</td><td>Butyrate to butyryl CoA pathway with IPTG inducible Promoter</td><td> Reversible reaction that makes Butyrate from Butyryl-CoA</td><td><a href="http://parts.igem.org/Part:BBa_K1618020">BBa_K1618020</a></td></tr>
 
−
 
−
<tr><td>Plac-THL-CRO-BHBD-BCD-EFTb-EFTa</td><td>pSB1C3</td><td>Acetyl-CoA to Butyrate pathway with IPTG inducible Promoter </td><td>Makes butyrate from Acetyl-CoA full</td><td><a href="http://parts.igem.org/Part:BBa_K1618021">BBa_K1618021</a></td></tr>
 
−
 
−
<tr><td>35S-MAO</td><td>?</td><td>Acylation candidate 1 with 35S Promoter</td><td>Acetylates starch? (1)</td><td><a href="http://parts.igem.org/Part:BBa_K1618033">BBa_K1618033</a></td></tr>
 
−
 
−
<tr><td>35S-Rv3030</td><td>?</td><td>Acylation candidate 2 with 35S Promoter</td><td>Acetylates starch? (2)</td><td><a href="http://parts.igem.org/Part:BBa_K1618034">BBa_K1618034</a></td></tr>
 
−
 
−
<tr><td>35S-Rv3034c</td><td>?</td><td>Acylation candidate 3 with 35S Promoter</td><td>Acetylates starch? (3)</td><td><a href="http://parts.igem.org/Part:BBa_K1618035">BBa_K1618035</a></td></tr>
 
−
 
−
<tr><td>35S-Rv3037c</td><td>?</td><td>Acylation candidate 4 with 35S Promoter</td><td>Acetylates starch? (4)</td><td><a href="http://parts.igem.org/Part:BBa_K1618036">BBa_K1618036</a></td></tr>
 
−
 
−
<tr><td>35S-GFP-MAO</td><td>?</td><td>Acylation candidate 1 with 35S Promoter + GFP</td><td>Acetylates starch? (1) + GFP tag</td><td><a href="http://parts.igem.org/Part:BBa_K1618029">BBa_K1618029</a></td>
 
−
</tr>
 
−
 
−
<tr><td>35S-GFP-Rv3030</td><td>?</td><td>Acylation candidate 2 with 35S Promoter + GFP</td><td>Acetylates starch? (2) + GFP tag</td><td><a href="http://parts.igem.org/Part:BBa_K1618030">BBa_K1618030</a></td></tr>
 
−
 
−
<tr><td>35S-GFP-Rv3034c</td><td>?</td><td>Acylation candidate 3 with 35S Promoter + GFP</td><td>Acetylates starch? (3) + GFP tag</td><td><a href="http://parts.igem.org/Part:BBa_K1618031">BBa_K1618031</a></td></tr>
 
−
 
−
<tr><td>35S-GFP-Rv3037c</td><td>?</td><td>Acylation candidate 4 with 35S Promoter + GFP</td><td>Acetylates starch? (4) + GFP tag</td><td><a href="http://parts.igem.org/Part:BBa_K1618032">BBa_K1618032</a></td></tr>
 
−
 
−
<tr><td>Chloroplast Transit Peptide</td><td>?</td><td>&nbsp;</td><td>Chloroplast Transit Peptide</td><td><a href="http://parts.igem.org/Part:BBa_K1618028">BBa_K1618028</a></td></tr>
 
−
 
−
<tr><td>35s Terminator</td><td>?</td><td>&nbsp;</td><td>Terminator</td><td><a href="http://parts.igem.org/Part:BBa_K1618037">BBa_K1618037</a></td></tr>
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
</table>
 
 
</body>
 
</body>
  

Revision as of 13:52, 16 September 2015

House of Carbs

Parts

BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents Backbone Description Function Part number
GlgXpSB1C3Debranching enzyme (E.coli)Coding sequence only BBa_K1618025
GlgBpSB1C3Branching Enzyme (E.coli)Coding sequence only BBa_K1618000
MAOpSB1C3Acyl-transferase Candidate 1 Coding sequence only BBa_K1618001
Rv3030pSB1C3Acylation candidate 2Coding sequence onlyBBa_K1618002
Rv3034cpSB1C3Acylation candidate 3Coding sequence only BBa_K1618003
Rv3037cpSB1C3Acylation candidate 4Coding sequence only BBa_K1618004

COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents Backbone Description Function Part number
P-Lac(R0010)_RBS(B0034)_GlgX(K1618026)pSB1C3Debranching enzyme expression constructRemoves glycogen branchesBBa_K1618026
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)pSB1C3Branching enzyme expression constructAdd glycogen branchesBBa_K1618022
P-Lac(R0010)_RBS(B0034)_MAO(K1618001)pSB1C3Acyl-transferase expression constructTransfers acyl groups to glycogenBBa_K1618005
P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002)pSB1C3Acyl-transferase expression constructTransfers acyl groups to glycogenBBa_K1618007
P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003)pSB1C3Acyl-transferase expression constructTransfers acyl groups to glycogen BBa_K1618008
P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004)pSB1C3Acyl-transferase expression constructTransfers acyl groups to glycogen BBa_K1618009
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_MAO(K1618001)pSB1C3Branching enzyme + acyl-transferase expression constructTransfers acyl groups to glycogenBBa_K1618023
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002)pSB1C3Branching enzyme + acyl-transferase expression construct)Transfers acyl groups to glycogenBBa_K1618023
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003)pSB1C3Branching enzyme + acyl-transferase expression constructTransfers acyl groups to glycogenBBa_K1618024
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004)pSB1C3BBranching enzyme + acyl-transferase expression constructTransfers acyl groups to glycogenBBa_K1618027

COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (CODING SEQUNCES WERE FROM Coprococcus sp. L2-50 (DSM 16842)

Part Contents Backbone Description Function Part number
P-Lac(R0010)_RBS(B0034)_BukpSB1C3Butyrate kinase expression contruct Catalyzes the conversionbutyrate to/from butyrylBBa_K1618012
P-Lac(R0010)_RBS(B0034)_PTBpSB1C3Phosphotransbutrylase expression constructCatalyzes the conversion butyryl to/from butyryl CoABBa_K1618013
P-Lac(R0010)_RBS(B0034)_Buk_PTBpSB1C3Butyrate to butyryl CoA pathway Catalyzes the conversion butyryl to/from butyryl CoABBa_K1618020
P-Lac(R0010)_RBS(B0034)_THLpSB1C3Thiolase expression constructCatalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA BBa_K1618014
P-Lac(R0010)_RBS(B0034)_CROpSB1C3Crotonase expression constructCatalyzes conversion of3-hydroxybutyryl-CoA to Crotonyl-CoA BBa_K1618015
P-Lac(R0010)_RBS(B0034)_BHBDpSB1C3β-hydroxybutyryl-CoA dehydrogenase expressionCatalyzes conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA BBa_K1618016
P-Lac(R0010)_RBS(B0034)_BCDpSB1C3Butyryl-CoA dehydrogenase expression construct Catalyzes conversion of Crotonyl-CoA to Butyryl-CoA BBa_K1618017
P-Lac(R0010)_RBS(B0034)_EFTbpSB1C3Electron transfer protein β expression constructCatalyzes conversion of acetate to butyrateBBa_K1618018
P-Lac(R0010)_RBS(B0034)_EFTapSB1C3Electron transfer protein α expression constructCatalyzes conversion of acetate to butyrateBBa_K1618019
P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBDpSB1C3Acetyl-CoA to 3-crotonyl-CoA pathwayCatalyzes conversion of Acetyl-CoA to 3-crotonyl-CoA--------
P-Lac(R0010)_RBS(B0034)_EFTapSB1C3Crotonyl-CoA to Butyryl-coA pathwayCatalyzes conversion of Crotonyl-CoA to Butyryl-coA-----------
P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTapSB1C3Acetyl-coA to Butryl-CoA pathwayCatalyzes conversion of Acetyl-coA to Butryl-CoA BBa_K1618021
P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTapSB1C3Acetyl coA to butyrate complete biosynthetic pathwayCatalyzes conversion of Acetyl coA to butyrate----------

COMPOUND PARTS FOR ENGINEERING PLANT STARCH

MANY THANKS TO OUR SPONSORS

Useful Links

Contact Us

  • nrpuea.igem2015@gmail.com

  • Norwich Research Park,
  • Colney,
  • Norwich, NR4 7UH, UK.

We are the NRP-UEA-Norwich 2015 iGEM Team.

Designed and developed by the NRP-UEA-Norwich iGEM Team

Part Contents Backbone Description Function Part number