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Revision as of 19:32, 16 September 2015
WETLAB
-Safety-
Safety First!
Saving the world starts with saving ourselves. You cannot save the world unless you’re in good shape. We put safety on the top priority
in any lab works. We have been informed accurately of the lab safety rules as well as bio-safety regulations at the
initial stage of the project in order to understand hazards and safety procedures. Also we received safety training from our instructor
before commencing our lab work. Even though our project is included Bio-Safety Level 1, we know very well that
there are still many risks that may be escalated into serious disaster. Furthermore, even when handling those risks that may not pose serious
threats to the experienced scientists, we as young learners should be extra careful.
The rule of thumb is:
1
Start with reading Safety Questions for overview.
2
Follow Elan Vital Safety Rules all the time.
3
Before entering the laboratory, read
GMS General Safety Rules
and GMS Extra Rules again and follow them
4
If you are unsure of the rules or confused,
ask lab assistant and/or
instructor before doing anything.
To The Top
We have conducted our experiments at Gachon University. Thus, we have always observed safety rules of the lab. We had Safety Education & Training Session
before starting our lab work. Also, when we conducted our experiments, Gachon University staff supervised our work.
Gachon University General Lab Safety Rule
1
200 mL LB prepared fresh,
non-autoclaved
2
3 g agar
3
Shake until all solids
are dissolved
4
Autoclave for 20 min within 2 hr
5
Keep it cool until it reaches
around 40-50 °C
6
Add 200 μL of 1000x chloramphenicol
and gently stir it. Be careful not
to shake the bottle too long/hard so that
bubbles are created.
7
Pour into empty petri dishes
just enough to cover the surface
(~20 mL per plate).
In case that bubbles are in
the plate, heat the plate surface
carefully with a burner only
until the bubbles are
burst but the solution is heated.
8
Leave the plates at room
temperature around one hour
until it is solidified.
9
Solidified plates should be
turned upside down for a
few hours at room temperature,
then stored at 4°C.