Difference between revisions of "Team:Tec-Chihuahua/Notebook"
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{{Tec-Chihuahua}} | {{Tec-Chihuahua}} | ||
− | <html> | + | <html lang="en"> |
+ | <head> | ||
+ | <meta charset="utf-8"> | ||
+ | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
+ | <title>Halcyon Days - An Exclusive Freebie for Codrops by Peter Finlan.</title> | ||
+ | <!-- | ||
+ | <link href="https://2015.igem.org/Team:Tec-Chihuahua/CSS?action=raw&ctype=text/css" rel="stylesheet"> | ||
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+ | DEBUG | ||
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+ | <link href="css/bootstrap.min.css" rel="stylesheet"> | ||
+ | <link href="css/flexslider.css" rel="stylesheet"> | ||
+ | <link href="css/styles.css" rel="stylesheet"> <!-- Rquiered --> | ||
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+ | <!-- HTML5 Shim and Respond.js IE8 support of HTML5 elements and media queries --> | ||
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+ | <script src="https://oss.maxcdn.com/libs/respond.js/1.4.2/respond.min.js"></script> | ||
+ | <![endif]--> | ||
− | < | + | </head> |
+ | <body id="top"> | ||
+ | |||
+ | <div id="float-menu"> | ||
+ | <ul> | ||
+ | <li><a href="index.html">Home</a></li> | ||
+ | <li><a href="team.html ">Team</a></li> | ||
+ | <li><a href="project.html">Project</a></li> | ||
+ | <li><a href="notebook.html">NoteBook</a></li> | ||
+ | <li><a href="parts.html">Parts</a></li> | ||
+ | <li><a href="safety.html">Safety</a></li> | ||
+ | <li><a href="modeling.html">Modeling</a></li> | ||
+ | <li><a href="hpractices.html">Human Practices</a></li> | ||
+ | <li><a href="collaboration.html">Collaboration</a></li> | ||
+ | <li class="float-menu-icon"><a href="https://www.facebook.com/iGemTecChih2015"><img src="img/smicons/fb.png"></a></li> | ||
+ | <li class="float-menu-icon"><a href="https://twitter.com/iGEM_TecChih"><img src="img/smicons/tt.png"></a></li> | ||
+ | <li class="float-menu-icon"><a href="http://instagram.com/igemtecchih "><img src="img/smicons/ig.png"></a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <header id="home"> | ||
+ | |||
+ | <section class="hero journal-background" id="hero"> | ||
+ | <div class="container"> | ||
+ | <div class="col-md-8 col-md-offset-2 text-center team-header"> | ||
+ | <h1 id="notebook-title">Notebook</h1> | ||
+ | |||
+ | </div> | ||
+ | <div class="col-md-7"> | ||
+ | <h1 id="safety-quote"></div> | ||
+ | <div class="col-md-5"> | ||
+ | <ul id="notebook-menu"> | ||
+ | <li><a href="#protocols">Protocols</a></li> | ||
+ | <li><a href="#journal">Journal</a></li> | ||
+ | <div class="col-md-6"> | ||
+ | <li><a class="notebook-month" href="#feb">Feb</a></li> | ||
+ | <li><a class="notebook-month" href="#apr">Apr</a></li> | ||
+ | <li><a class="notebook-month" href="#jun">Jun</a></li> | ||
− | < | + | </div> |
+ | <div class="col-md-6"> | ||
+ | <li><a class="notebook-month" href="#mar">Mar</a></li> | ||
+ | <li><a class="notebook-month" href="#may">May</a></li> | ||
+ | <li><a class="notebook-month" href="#jul">Jul</a></li> | ||
− | < | + | </div> |
− | <ul> | + | |
− | <li> | + | </ul> |
− | <li> | + | |
− | <li> | + | </div> |
− | <li> | + | </div> |
− | </ul> | + | </section> |
+ | </header> | ||
+ | <section class="text-center"> | ||
+ | <div class="container"> | ||
+ | |||
+ | <h1 id="protocols" class="arrow">Protocols</h1> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 protocol-left"> | ||
+ | <h2>CNT</h2> | ||
+ | <div class="col-md-12"> | ||
+ | <ul> | ||
+ | <li><a href="/assets/protocols/sintesisCNT.pdf" target="_blank">Sintesis CNT<img src="img/pdfico.png" ></a></li> | ||
+ | <li><a href="/assets/protocols/sintesisCNT.pdf" target="_blank">Characterization CNT<img src="img/pdfico.png" ></a></li> | ||
+ | <li><a href="/assets/protocols/sintesisCNT.pdf" target="_blank">Purification CNT<img src="img/pdfico.png" ></a></li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-6 protocol-right"> | ||
+ | <h2>Ocytes</h2> | ||
+ | <div class="col-md-12"> | ||
+ | <ul> | ||
+ | <li><a href="#" target="_blank"><img src="img/pdfico.png" >Vacuuming, selection and Maduration</a></li> | ||
+ | <li><a href="#" target="_blank"><img src="img/pdfico.png" >Competent Cells</a></li> | ||
+ | <li><a href="#" target="_blank"><img src="img/pdfico.png" >Cell Transformation</a></li> | ||
+ | <li><a href="#" target="_blank"><img src="img/pdfico.png" >Internalization Studies</a></li> | ||
+ | <li><a href="#" target="_blank"><img src="img/pdfico.png" >Fixation</a></li> | ||
+ | <li><a href="#" target="_blank"><img src="img/pdfico.png" >Interaction</a></li> | ||
+ | <li><a href="#" target="_blank"><img src="img/pdfico.png" >DAPI</a></li> | ||
+ | |||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-6 protocol-left"> | ||
+ | <h2>Parts</h2> | ||
+ | <div class="col-md-12"> | ||
+ | <ul> | ||
+ | <li><a href="#" target="_blank">Assembling<img src="img/pdfico.png" ></a></li> | ||
+ | <li><a href="#" target="_blank">Digestion<img src="img/pdfico.png" ></a></li> | ||
+ | <li><a href="#" target="_blank">MiniPrep<img src="img/pdfico.png" ></a></li> | ||
+ | |||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-6 protocol-right"> | ||
+ | <h2>Plants</h2> | ||
+ | <div class="col-md-12"> | ||
+ | <ul> | ||
+ | |||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <!-- START OF THE JOURNAL!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! --> | ||
+ | <h1 id="journal" class="arrow">Journal</h1> | ||
+ | <h2 id="feb" >February</h2> | ||
+ | <div class="row"> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Feb</b> / 26 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>First meeting of the team. We talked about the proyect and started generating ideas.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <h2 id="mar">March</h2 > | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Mar</b> / 4 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Choice of the project.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Mar</b> / 14 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We won the Entrepreneurship Contest of the Nucleum Congress in Monterrey.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Mar</b> / 19 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Team Meeting.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Mar</b> / 26 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Team promotional photo.</p> | ||
+ | <img src="img/Journal-photos/13262015.jpg"> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <h2 id="apr">April</h2> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Abr</b> / 2 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Team Meeting</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Abr</b> / 9 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We presented our ideas to experts of the Centro de Investigación en Materiales Avanzados (CIMAV), they seem interested on the Project and gave us their support. </p> | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Abr</b> / 17 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We went to the local TV Show “Emma de la O” to talk about the biotechnology and our project.</p> | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Abr</b> / 20 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Synthesis of the NP-CNT. We began the synthesis of the nanotubes at CIMAV.</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <h2 id="may">May</h2> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>May</b> / 12 - 15 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Purification of the NP-CNT</p> | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>May</b> / 20 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Filtration of the P-CNT</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>May</b> / 21 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Preparation of Luria Bertani Broth for <span class="bacteria">E. coli</span></p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>May</b> / 22 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We finished the filtration of the nanotubes</p> | ||
+ | <p>Plasmid extraction of <span class="bacteria">E. coli</span></p> | ||
+ | # | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>May</b> / 25 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We measured the purity of plasmid using the NanoDrop 2000</p> | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>May</b> / 27 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Team meeting to discuss about the wiki</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>May</b> / 28 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Inventory of team´s material and lab cleanup.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <h2 id="jun">June</h2> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 1 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Preparation of Luria Bertani agar.</p> | ||
+ | <p>Team meeting for fund collection.</p> | ||
+ | <p>Sterilizing of work solutions.</p> | ||
+ | <p>We began to dry the P-CNT</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 3 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We finished drying the nanotubes.</p> | ||
+ | <p>Preparation of PBS</p> | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 8 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We began the work with the bovine oocytes at the Facultad de Zootecnia (School of Animal Husbandry) of the Universidad Autónoma de Chihuahua (UACH).</p> | ||
+ | # | ||
+ | # | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 9 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Arriving of the Florescein Diacetate.</p> | ||
+ | <p>Oocytes extraction and selection.</p> | ||
+ | <p>The maduration of the viables oocytes began.</p> | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 10 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We began the CNT-Fluorescein functionalization.</p> | ||
+ | <p>Cleaning and picking of the oocytes.</p> | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 11 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We finished the CNT functionalization with fluorescein.</p> | ||
+ | # | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 15 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Pins Ceremony</p> | ||
+ | # | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 16 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>F-NTC / Oocytes interaction. We failed </p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 18 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Purification of the Second batch of CNT.</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 22 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Preparation of the stocks and the media for the handling and maduration of oocytes.</p> | ||
+ | # | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 24 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Oocytes extraction and selection.</P> | ||
+ | <p>The maduration of the viables oocytes began.</p> | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jun</b>/ 25 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Cleaning and picking of the oocytes.</p> | ||
+ | <p>F-NTC / Oocytes interaction.</p> | ||
+ | <p>Filtration of the second group of nanotubes</p> | ||
+ | # | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 26 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>“About our lab” and “About our proyect” Questionnaires.</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 29 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Team meeting.</p> | ||
+ | <p>Observation of functionalized oocytes with confocal microscope.</p> | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jun</b> / 30 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Filtration of the CNT.</p> | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <h2 id="jul">July</h2> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 1 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We finished the filtration of the second batch of CNT.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 2 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Drying of the P-CNT.</p> | ||
+ | <p>Oocytes extraction and selection.</p> | ||
+ | <p>Preparation of work solutions.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 3 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Preparation of work solutions.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 4 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>F-NTC / Oocytes interaction and staining of the oocytes with DAPI.</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 6 /2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Team meeting</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 7 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Presentation to high school students about Synthetic Biology for the Human Practices.</p> | ||
+ | # | ||
+ | # | ||
+ | # | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 8 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Oocytes extraction and selection for maduration</p> | ||
+ | <p>We fabricated glass pipettes</p> | ||
+ | <p>Wiki meeting with Sammy.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 9 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Cleaning and picking of the oocytes.</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 10 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>F-NTC / Oocytes interaction and staining of the oocytes with DAPI.</p> | ||
+ | # | ||
+ | # | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 13 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We took a Biosafety Course for the Safety requirements.</p> | ||
+ | <p>Wiki meeting with Sammy</p> | ||
+ | # | ||
+ | # | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 15 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Team meeting.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 16 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Wiki meeting with Sammy.</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 17 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Preparation of work solutions.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 20 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Meeting with Dr. Quintín Rascon to planning the work on <span class="bacteria"> N. tabacum.</span></p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 21 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We started Functionalization with Fluorescein of the second batch of P-CNT.</p> | ||
+ | <p>Oocytes extraction and selection for maduration.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 22 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>We finished the CNT-functionalization.</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 23 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Preparation of solutions.</p> | ||
+ | <p>Interaction of the oocytes with the F-CNT (both of the batches), previously fixed with paraformaldehyde.</p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 24 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Team meeting</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Jul</b> / 27 - 31 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Research and documentary work.</p> | ||
+ | <p>Working on the wiki content.</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <h2>August</h2> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Aug</b> / 03 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Wiki Content work</p> | ||
+ | <p>Team meating</p> | ||
+ | </div> | ||
− | + | </div> | |
− | + | </div> | |
− | < | + | <div class="col-md-12"> |
− | < | + | <div class="col-md-6 col-md-offset-6 journal-right"> |
− | < | + | <h3 class="quote-date"><b>Aug</b> / 04 - 05 / 2015</h3> |
− | + | <div class="col-md-12 in-quote"> | |
− | < | + | <p>Wiki meeting with Sammy</p> |
− | + | ||
− | </div> | + | </div> |
− | </html> | + | |
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Aug</b> / 06 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>we grew <span class="bacteria">E. coli</span> TOP10 on LB brith and incubated it on shaker</p> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Aug</b> / 07 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Sterilizing solutions</p> | ||
+ | <p>Aliquots of <span class="bacteria">E. coli</span> and glycerol were prepared</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Aug</b> / 10 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Team meeting</p> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Aug</b> / 11 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Preparation of Solutions</p> | ||
+ | <p>Preparation of LB Broth</p> | ||
+ | <p>The optical density of TOP10 was measured</p> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 journal-left"> | ||
+ | <h3 class="quote-date"><b>Aug</b> / 12 - 14 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Biobricks cloning started: We failed on cell transformation</p> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <h2>September</h2> | ||
+ | <div class="col-md-12"> | ||
+ | <div class="col-md-6 col-md-offset-6 journal-right"> | ||
+ | <h3 class="quote-date"><b>Sep</b> / 04 - 18 / 2015</h3> | ||
+ | <div class="col-md-12 in-quote"> | ||
+ | <p>Lab work with the biobricks</p> | ||
+ | <p>Fast and Furiously Finishing the Wiki</p> | ||
+ | <p>Last details of presentation and poster</p> | ||
+ | <p>Making of promotional video.</p> | ||
+ | </br> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </br> | ||
+ | <h1 class="arrow">Months of work finally come to end!</h1> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <!-- END OF THE JOURNAL!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! --> | ||
+ | </div> | ||
+ | </div> | ||
+ | </section> | ||
+ | <section class="intro text-center" id="intro"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </section> | ||
+ | |||
+ | <footer class="text-center"> | ||
+ | <div class="col-md-8 col-md-offset-2"> | ||
+ | <div class="col-md-4"> | ||
+ | <p>Address</p> | ||
+ | <p>Av. Heróico Colegio Militar 4700 Col. Nombre de Dios, Zip Code: 31300 </p> | ||
+ | </div> | ||
+ | <div class="col-md-4"> | ||
+ | <p>Phone Number</p> | ||
+ | <p>+52 (614) 439 5000 (Ext. 3009)</p> | ||
+ | </div> | ||
+ | <div class="col-md-4"> | ||
+ | <p>Email</p> | ||
+ | <p>igem_chih@outlook.com</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </footer> | ||
+ | <!-- jQuery (necessary for Bootstrap's JavaScript plugins) --> | ||
+ | <script src="https://ajax.googleapis.com/ajax/libs/jquery/1.11.0/jquery.min.js"></script> | ||
+ | <!-- Include all compiled plugins (below), or include individual files as needed --> | ||
+ | <script src="js/waypoints.min.js"></script> | ||
+ | <script src="js/bootstrap.min.js"></script> | ||
+ | <script src="js/scripts.js"></script> | ||
+ | <script src="js/jquery.flexslider.js"></script> | ||
+ | <script src="js/modernizr.js"></script> | ||
+ | </body> | ||
+ | </html> |
Revision as of 20:06, 16 September 2015
Notebook
Protocols
Ocytes
Parts
Plants
Journal
February
Feb / 26 / 2015
First meeting of the team. We talked about the proyect and started generating ideas.
March
Mar / 4 / 2015
Choice of the project.
Mar / 14 / 2015
We won the Entrepreneurship Contest of the Nucleum Congress in Monterrey.
Mar / 19 / 2015
Team Meeting.
Mar / 26 / 2015
Team promotional photo.
April
Abr / 2 / 2015
Team Meeting
Abr / 9 / 2015
We presented our ideas to experts of the Centro de Investigación en Materiales Avanzados (CIMAV), they seem interested on the Project and gave us their support.
#Abr / 17 / 2015
We went to the local TV Show “Emma de la O” to talk about the biotechnology and our project.
#Abr / 20 / 2015
Synthesis of the NP-CNT. We began the synthesis of the nanotubes at CIMAV.
May
May / 12 - 15 / 2015
Purification of the NP-CNT
#May / 20 / 2015
Filtration of the P-CNT
May / 21 / 2015
Preparation of Luria Bertani Broth for E. coli
May / 22 / 2015
We finished the filtration of the nanotubes
Plasmid extraction of E. coli
# #May / 25 / 2015
We measured the purity of plasmid using the NanoDrop 2000
#May / 27 / 2015
Team meeting to discuss about the wiki
May / 28 / 2015
Inventory of team´s material and lab cleanup.
June
Jun / 1 / 2015
Preparation of Luria Bertani agar.
Team meeting for fund collection.
Sterilizing of work solutions.
We began to dry the P-CNT
Jun / 3 / 2015
We finished drying the nanotubes.
Preparation of PBS
#Jun / 8 / 2015
We began the work with the bovine oocytes at the Facultad de Zootecnia (School of Animal Husbandry) of the Universidad Autónoma de Chihuahua (UACH).
# #Jun / 9 / 2015
Arriving of the Florescein Diacetate.
Oocytes extraction and selection.
The maduration of the viables oocytes began.
#Jun / 10 / 2015
We began the CNT-Fluorescein functionalization.
Cleaning and picking of the oocytes.
#Jun / 11 / 2015
We finished the CNT functionalization with fluorescein.
# #Jun / 15 / 2015
Pins Ceremony
#Jun / 16 / 2015
F-NTC / Oocytes interaction. We failed
Jun / 18 / 2015
Purification of the Second batch of CNT.
Jun / 22 / 2015
Preparation of the stocks and the media for the handling and maduration of oocytes.
# #Jun / 24 / 2015
Oocytes extraction and selection.
The maduration of the viables oocytes began.
#Jun/ 25 / 2015
Cleaning and picking of the oocytes.
F-NTC / Oocytes interaction.
Filtration of the second group of nanotubes
# #Jun / 26 / 2015
“About our lab” and “About our proyect” Questionnaires.
Jun / 29 / 2015
Team meeting.
Observation of functionalized oocytes with confocal microscope.
#Jun / 30 / 2015
Filtration of the CNT.
#July
Jul / 1 / 2015
We finished the filtration of the second batch of CNT.
Jul / 2 / 2015
Drying of the P-CNT.
Oocytes extraction and selection.
Preparation of work solutions.
Jul / 3 / 2015
Preparation of work solutions.
Jul / 4 / 2015
F-NTC / Oocytes interaction and staining of the oocytes with DAPI.
Jul / 6 /2015
Team meeting
Jul / 7 / 2015
Presentation to high school students about Synthetic Biology for the Human Practices.
# # #Jul / 8 / 2015
Oocytes extraction and selection for maduration
We fabricated glass pipettes
Wiki meeting with Sammy.
Jul / 9 / 2015
Cleaning and picking of the oocytes.
Jul / 10 / 2015
F-NTC / Oocytes interaction and staining of the oocytes with DAPI.
# #Jul / 13 / 2015
We took a Biosafety Course for the Safety requirements.
Wiki meeting with Sammy
# #Jul / 15 / 2015
Team meeting.
Jul / 16 / 2015
Wiki meeting with Sammy.
Jul / 17 / 2015
Preparation of work solutions.
Jul / 20 / 2015
Meeting with Dr. Quintín Rascon to planning the work on N. tabacum.
Jul / 21 / 2015
We started Functionalization with Fluorescein of the second batch of P-CNT.
Oocytes extraction and selection for maduration.
Jul / 22 / 2015
We finished the CNT-functionalization.
Jul / 23 / 2015
Preparation of solutions.
Interaction of the oocytes with the F-CNT (both of the batches), previously fixed with paraformaldehyde.
Jul / 24 / 2015
Team meeting
Jul / 27 - 31 / 2015
Research and documentary work.
Working on the wiki content.
August
Aug / 03 / 2015
Wiki Content work
Team meating
Aug / 04 - 05 / 2015
Wiki meeting with Sammy
Aug / 06 / 2015
we grew E. coli TOP10 on LB brith and incubated it on shaker
Aug / 07 / 2015
Sterilizing solutions
Aliquots of E. coli and glycerol were prepared
Aug / 10 / 2015
Team meeting
Aug / 11 / 2015
Preparation of Solutions
Preparation of LB Broth
The optical density of TOP10 was measured
Aug / 12 - 14 / 2015
Biobricks cloning started: We failed on cell transformation
September
Sep / 04 - 18 / 2015
Lab work with the biobricks
Fast and Furiously Finishing the Wiki
Last details of presentation and poster
Making of promotional video.