Difference between revisions of "Team:Tec-Chihuahua/Notebook"

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                                 <li><a href="/assets/protocols/sintesisCNT.pdf" target="_blank">Synthesis and Purification Purification CNT<img src="img/pdfico.png" ></a></li>
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Revision as of 22:37, 16 September 2015

Carbon carriers Halcyon Days - An Exclusive Freebie for Codrops by Peter Finlan.

Protocols

Journal

February

Feb / 26 / 2015

First meeting of the team. We talked about the proyect and started generating ideas.

March

Mar / 4 / 2015

Choice of the project.

Mar / 14 / 2015

We won the Entrepreneurship Contest of the Nucleum Congress in Monterrey.

Mar / 19 / 2015

Team Meeting.

Mar / 26 / 2015

Team promotional photo.

April

Abr / 2 / 2015

Team Meeting

Abr / 9 / 2015

We presented our ideas to experts of the Centro de Investigación en Materiales Avanzados (CIMAV), they seem interested on the Project and gave us their support.

#

Abr / 17 / 2015

We went to the local TV Show “Emma de la O” to talk about the biotechnology and our project.

#

Abr / 20 / 2015

Synthesis of the NP-CNT. We began the synthesis of the nanotubes at CIMAV.

May

May / 12 - 15 / 2015

Purification of the NP-CNT

#

May / 20 / 2015

Filtration of the P-CNT

May / 21 / 2015

Preparation of Luria Bertani Broth for E. coli

May / 22 / 2015

We finished the filtration of the nanotubes

Plasmid extraction of E. coli

# #

May / 25 / 2015

We measured the purity of plasmid using the NanoDrop 2000

#

May / 27 / 2015

Team meeting to discuss about the wiki

May / 28 / 2015

Inventory of team´s material and lab cleanup.

June

Jun / 1 / 2015

Preparation of Luria Bertani agar.

Team meeting for fund collection.

Sterilizing of work solutions.

We began to dry the P-CNT

Jun / 3 / 2015

We finished drying the nanotubes.

Preparation of PBS

#

Jun / 8 / 2015

We began the work with the bovine oocytes at the Facultad de Zootecnia (School of Animal Husbandry) of the Universidad Autónoma de Chihuahua (UACH).

# #

Jun / 9 / 2015

Arriving of the Florescein Diacetate.

Oocytes extraction and selection.

The maduration of the viables oocytes began.

#

Jun / 10 / 2015

We began the CNT-Fluorescein functionalization.

Cleaning and picking of the oocytes.

#

Jun / 11 / 2015

We finished the CNT functionalization with fluorescein.

# #

Jun / 15 / 2015

Pins Ceremony

#

Jun / 16 / 2015

F-NTC / Oocytes interaction. We failed

Jun / 18 / 2015

Purification of the Second batch of CNT.

Jun / 22 / 2015

Preparation of the stocks and the media for the handling and maduration of oocytes.

# #

Jun / 24 / 2015

Oocytes extraction and selection.

The maduration of the viables oocytes began.

#

Jun/ 25 / 2015

Cleaning and picking of the oocytes.

F-NTC / Oocytes interaction.

Filtration of the second group of nanotubes

# #

Jun / 26 / 2015

“About our lab” and “About our proyect” Questionnaires.

Jun / 29 / 2015

Team meeting.

Observation of functionalized oocytes with confocal microscope.

#

Jun / 30 / 2015

Filtration of the CNT.

#

July

Jul / 1 / 2015

We finished the filtration of the second batch of CNT.

Jul / 2 / 2015

Drying of the P-CNT.

Oocytes extraction and selection.

Preparation of work solutions.

Jul / 3 / 2015

Preparation of work solutions.

Jul / 4 / 2015

F-NTC / Oocytes interaction and staining of the oocytes with DAPI.

Jul / 6 /2015

Team meeting

Jul / 7 / 2015

Presentation to high school students about Synthetic Biology for the Human Practices.

# # #

Jul / 8 / 2015

Oocytes extraction and selection for maduration

We fabricated glass pipettes

Wiki meeting with Sammy.

Jul / 9 / 2015

Cleaning and picking of the oocytes.

Jul / 10 / 2015

F-NTC / Oocytes interaction and staining of the oocytes with DAPI.

# #

Jul / 13 / 2015

We took a Biosafety Course for the Safety requirements.

Wiki meeting with Sammy

# #

Jul / 15 / 2015

Team meeting.

Jul / 16 / 2015

Wiki meeting with Sammy.

Jul / 17 / 2015

Preparation of work solutions.

Jul / 20 / 2015

Meeting with Dr. Quintín Rascon to planning the work on N. tabacum.

Jul / 21 / 2015

We started Functionalization with Fluorescein of the second batch of P-CNT.

Oocytes extraction and selection for maduration.

Jul / 22 / 2015

We finished the CNT-functionalization.

Jul / 23 / 2015

Preparation of solutions.

Interaction of the oocytes with the F-CNT (both of the batches), previously fixed with paraformaldehyde.

Jul / 24 / 2015

Team meeting

Jul / 27 - 31 / 2015

Research and documentary work.

Working on the wiki content.

August

Aug / 03 / 2015

Wiki Content work

Team meating

Aug / 04 - 05 / 2015

Wiki meeting with Sammy

Aug / 06 / 2015

we grew E. coli TOP10 on LB brith and incubated it on shaker

Aug / 07 / 2015

Sterilizing solutions

Aliquots of E. coli and glycerol were prepared

Aug / 10 / 2015

Team meeting

Aug / 11 / 2015

Preparation of Solutions

Preparation of LB Broth

The optical density of TOP10 was measured

Aug / 12 - 14 / 2015

Biobricks cloning started: We failed on cell transformation

September

Sep / 04 - 18 / 2015

Lab work with the biobricks

Fast and Furiously Finishing the Wiki

Last details of presentation and poster

Making of promotional video.



Months of work finally come to end!

Address

Av. Heróico Colegio Militar 4700 Col. Nombre de Dios, Zip Code: 31300

Phone Number

+52 (614) 439 5000 (Ext. 3009)

Email

igem_chih@outlook.com