Team:Tec-Chihuahua/Notebook
Notebook
Protocols
Oocytes
Parts
Plants
Journal
Feb / 26 / 2015
First meeting of the team. We talked about the proyect and started generating ideas.
Mar / 4 / 2015
Choice of the project.
Mar / 14 / 2015
We won the Entrepreneurship Contest of the Nucleum Congress in Monterrey.
Mar / 19 / 2015
Team Meeting.
Mar / 26 / 2015
Team promotional photo.
Abr / 2 / 2015
Team Meeting
Abr / 9 / 2015
We presented our ideas to experts of the Centro de Investigación en Materiales Avanzados (CIMAV), they seem interested on the Project and gave us their support.
Abr / 17 / 2015
We went to the local TV Show “Emma de la O” to talk about the biotechnology and our project.
Abr / 20 / 2015
Synthesis of the NP-CNT. We began the synthesis of the nanotubes at CIMAV.
May / 12 - 15 / 2015
Purification of the NP-CNT
May / 20 / 2015
Filtration of the P-CNT
May / 21 / 2015
Preparation of Luria Bertani Broth for E. coli
May / 22 / 2015
We finished the filtration of the nanotubes
Plasmid extraction of E. coli
May / 25 / 2015
We measured the purity of plasmid using the NanoDrop 2000
May / 27 / 2015
Team meeting to discuss about the wiki
May / 28 / 2015
Inventory of team´s material and lab cleanup.
Jun / 1 / 2015
Preparation of Luria Bertani agar.
Team meeting for fund collection.
Sterilizing of work solutions.
We began to dry the P-CNT
Jun / 3 / 2015
We finished drying the nanotubes.
Preparation of PBS
Jun / 8 / 2015
We began the work with the bovine oocytes at the Facultad de Zootecnia (School of Animal Husbandry) of the Universidad Autónoma de Chihuahua (UACH).
Jun / 9 / 2015
Arriving of the Florescein Diacetate.
Oocytes extraction and selection.
The maduration of the viables oocytes began.
Jun / 10 / 2015
We began the CNT-Fluorescein functionalization.
Cleaning and picking of the oocytes.
Jun / 11 / 2015
We finished the CNT functionalization with fluorescein.
Jun / 15 / 2015
Pins Ceremony
Jun / 16 / 2015
F-NTC / Oocytes interaction. We failed
Jun / 18 / 2015
Purification of the Second batch of CNT.
Jun / 22 / 2015
Preparation of the stocks and the media for the handling and maduration of oocytes.
Jun / 24 / 2015
Oocytes extraction and selection.
The maduration of the viables oocytes began.
Jun/ 25 / 2015
Cleaning and picking of the oocytes.
F-NTC / Oocytes interaction.
Filtration of the second group of nanotubes
Jun / 26 / 2015
“About our lab” and “About our proyect” Questionnaires.
Jun / 29 / 2015
Team meeting.
Observation of functionalized oocytes with confocal microscope.
Jun / 30 / 2015
Filtration of the CNT.
Jul / 1 / 2015
We finished the filtration of the second batch of CNT.
Jul / 2 / 2015
Drying of the P-CNT.
Oocytes extraction and selection.
Preparation of work solutions.
Jul / 3 / 2015
Preparation of work solutions.
Jul / 4 / 2015
F-NTC / Oocytes interaction and staining of the oocytes with DAPI.
Jul / 6 /2015
Team meeting
Jul / 7 / 2015
Presentation to high school students about Synthetic Biology for the Human Practices.
Jul / 8 / 2015
Oocytes extraction and selection for maduration
We fabricated glass pipettes
Wiki meeting with Sammy.
Jul / 9 / 2015
Cleaning and picking of the oocytes.
Jul / 10 / 2015
F-NTC / Oocytes interaction and staining of the oocytes with DAPI.
Jul / 13 / 2015
We took a Biosafety Course for the Safety requirements.
Wiki meeting with Sammy
Jul / 15 / 2015
Team meeting.
Jul / 16 / 2015
Wiki meeting with Sammy.
Jul / 17 / 2015
Preparation of work solutions.
Jul / 20 / 2015
Meeting with Dr. Quintín Rascon to planning the work on N. tabacum.
Jul / 21 / 2015
We started Functionalization with Fluorescein of the second batch of P-CNT.
Oocytes extraction and selection for maduration.
Jul / 22 / 2015
We finished the CNT-functionalization.
Jul / 23 / 2015
Preparation of solutions.
Interaction of the oocytes with the F-CNT (both of the batches), previously fixed with paraformaldehyde.
Jul / 24 / 2015
Team meeting
Jul / 27 - 31 / 2015
Research and documentary work.
Working on the wiki content.
Aug / 03 / 2015
Wiki Content work
Team meating
Aug / 04 - 05 / 2015
Wiki meeting with Sammy
Aug / 06 / 2015
we grew E. coli TOP10 on LB brith and incubated it on shaker
Aug / 07 / 2015
Sterilizing solutions
Aliquots of E. coli and glycerol were prepared
Aug / 10 / 2015
Team meeting
Aug / 11 / 2015
Preparation of Solutions
Preparation of LB Broth
The optical density of TOP10 was measured
Aug / 12 - 14 / 2015
Biobricks cloning started: We failed on cell transformation
Sep / 04 - 18 / 2015
Lab work with the biobricks
Fast and Furiously Finishing the Wiki
Last details of presentation and poster
Making of promotional video.
