Difference between revisions of "Team:CU Boulder/project/motivation"

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<br><br>
 
<br><br>
  
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<h2>General statement of modeling our product as the next hi-tech
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<h2>The CU Boulder iGEM team was inspired by combining ideas from previous work and legislation related to fracking with recently-produced genetics including the use of logic gates and cell-to-cell signaling. This combination ultimately results in the most cutting-edge naphthalene biosensor for practical industrial use in controlling groundwater contamination due to fracking.</h2>
−
step necessary in industry</h2>
+
 
<p>Overall history of fracking, beginning with origins, discussion of general attitudes and
 
<p>Overall history of fracking, beginning with origins, discussion of general attitudes and
 
motivations within the industries, describe general end goal that people are working on
 
motivations within the industries, describe general end goal that people are working on
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<div class="date">2004</div><div class="fact_title fact_2004">Early Engineering of Whole Cell Biosensors</div>
 
<div class="date">2004</div><div class="fact_title fact_2004">Early Engineering of Whole Cell Biosensors</div>
 
<div class="fact_box fact_2004">
 
<div class="fact_box fact_2004">
−
     <p>Description of event with 8 components. 1. Motivation behind the
+
     <p>The first description of a whole-cell biosensor with environmental applications was described by researchers at the Swiss Federal Institute for Environmental Science and Technology. They engineered Pseudomonas putida bacteria to luminesce proportionately to aqueous naphthalene concentrations. However, these bacteria had complex biochemical pathways that were not well enough understood to be easily adjusted and refined. Furthermore, the cells were not sensitive enough to report the dilute naphthalene concentrations that fracking companies may be interested in reporting.</p>
−
action. 2. Who did it and why was it them. 3. What was accomplished.
+
−
4. Why was it accomplished. 5. What did it do that was so novel. 6.
+
−
Who benefited the most. 7. what questions did it leave unanswered. 8.
+
−
Quote from some source. I’m in the process of filling out all these in a
+
−
separate word document. each of the paragraphs will be approximately
+
−
7 lines in length so this is just filler text so the formatting is easy later.</p>
+
 
</div>
 
</div>
 
<br><br>
 
<br><br>
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<div class="date">2005</div><div class="fact_title fact_2005">Bush Signs Energy Policy Act</div>
 
<div class="date">2005</div><div class="fact_title fact_2005">Bush Signs Energy Policy Act</div>
 
<div class="fact_box fact_2005">
 
<div class="fact_box fact_2005">
−
     <p>Description of event with 8 components. 1. Motivation behind the
+
     <p>The Energy Policy Act of 2005 was passed to change US energy regulations, and restructuring tax incentives within the energy industries. Part of this bill included a provision that would exempt natural gas companies that use fracking from disclosing the chemicals involved that would normally be required of energy companies. While many drilling companies would benefit, it caused the started to grow skeptical about their intentions and the environmental impact of hydraulic fracturing.</p>
−
action. 2. Who did it and why was it them. 3. What was accomplished.
+
−
4. Why was it accomplished. 5. What did it do that was so novel. 6.
+
−
Who benefited the most. 7. what questions did it leave unanswered. 8.
+
−
Quote from some source. I’m in the process of filling out all these in a
+
−
separate word document. each of the paragraphs will be approximately
+
−
7 lines in length so this is just filler text so the formatting is easy later.</p>
+
 
</div>
 
</div>
 
<br><br>
 
<br><br>
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<div class="date">2011</div><div class="fact_title fact_2011">Founding of FracFocus Chemical Disclosure Agency</div>
 
<div class="date">2011</div><div class="fact_title fact_2011">Founding of FracFocus Chemical Disclosure Agency</div>
 
<div class="fact_box fact_2011">
 
<div class="fact_box fact_2011">
−
<p>Description of event with 8 components. 1.
+
<p>According to their website, FracFocus Chemical Disclosure is a government run agency that was the first to provide public access to information on fracking and the chemicals used near homes. Naphthalene was reported as the most common, as it was found in more than 30% of all contamination sites. Recent legislation has required companies to register the chemicals they use on FracFocus, and it coordinates with reporting companies to keep updated records of contaminant history across the United States. The foundation of the site and coordinating legislation provided transparency, but few companies have been held accountable for data published through FracFocus.</p>
−
Motivation behind the action. 2. Who did it
+
−
and why was it them. 3. What was accomplished.
+
−
4. Why was it accomplished. 5. What
+
−
did it do that was so novel.
+
−
6. Who benefitted the most. 7. what questions
+
−
did it leave unanswered. 8. Quote from some
+
−
source. I’m in the process of filling out all
+
−
these in a separate word document. each of
+
−
the paragraphs will be approcimately 7 lines
+
−
in length so this is just filler text so the formatting
+
−
is easy later.</p>
+
 
</div>
 
</div>
 
<br><br>
 
<br><br>
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<div class="date">2012</div><div class="fact_title fact_2012">OptiEnz Sensors Begin Use</div>
 
<div class="date">2012</div><div class="fact_title fact_2012">OptiEnz Sensors Begin Use</div>
 
<div class="fact_box fact_2012">
 
<div class="fact_box fact_2012">
−
<p>Description of event with 8 components. 1.
+
<p>Since the government had loose regulations on fracking contamination and people grew more concerned about the now-reported chemicals being used, the private sector’s demand for chemical sensors increased. OptiEnz is a leading biosensor development company that was founded by a professor at Colorado State University. Their product uses extracted enzymes and fiber optics to sense real-time pollution directly in the water source, preventing the need for pretreatment and skilled labor that has been previously required.</p>
−
Motivation behind the action. 2. Who did it
+
−
and why was it them. 3. What was accomplished.
+
−
4. Why was it accomplished. 5. What
+
−
did it do that was so novel.
+
−
6. Who benefitted the most. 7. what questions
+
−
did it leave unanswered. 8. Quote from some
+
−
source. I’m in the process of filling out all
+
−
these in a separate word document. each of
+
−
the paragraphs will be approcimately 7 lines
+
−
in length so this is just filler text so the formatting
+
−
is easy later.</p>
+
 
</div>
 
</div>
 
<br><br>
 
<br><br>
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<div class="date">2013</div><div class="fact_title fact_2013">Peking iGEM Team Improves Aromatic Transcriptional Regulators</div>
 
<div class="date">2013</div><div class="fact_title fact_2013">Peking iGEM Team Improves Aromatic Transcriptional Regulators</div>
 
<div class="fact_box fact_2013">
 
<div class="fact_box fact_2013">
−
<p>Description of event with 8 components. 1.
+
<p>Based on information from their wiki, the 2013 iGEM team from Peking developed many parts to detect aromatic compounds. Among those produced were the NahR transcriptional regulator combined with a salicylate promoter and an sfGFP gene that can respond to naphthalene at concentrations within an order of magnitude of the EPA’s maximum of 20 µg/L. This part was thoroughly characterized and available in the distribution kit, so it quickly became the goal of the CU 2015 team to improve its use to make the system more sensitive, easier to use, and ultimately more practical for industrial application.</p>
−
Motivation behind the action. 2. Who did it
+
−
and why was it them. 3. What was accomplished.
+
−
4. Why was it accomplished. 5. What
+
−
did it do that was so novel.
+
−
6. Who benefitted the most. 7. what questions
+
−
did it leave unanswered. 8. Quote from some
+
−
source. I’m in the process of filling out all
+
−
these in a separate word document. each of
+
−
the paragraphs will be approcimately 7 lines
+
−
in length so this is just filler text so the formatting
+
−
is easy later.</p>
+
 
</div>
 
</div>
 
<br><br>
 
<br><br>

Revision as of 02:53, 17 September 2015


<!DOCTYPE html> Team:CU_Boulder - 2015.igem.org

Motivation

A BIG FRACKING DEAL

some intro





Summary of the video



Insert timeline



The CU Boulder iGEM team was inspired by combining ideas from previous work and legislation related to fracking with recently-produced genetics including the use of logic gates and cell-to-cell signaling. This combination ultimately results in the most cutting-edge naphthalene biosensor for practical industrial use in controlling groundwater contamination due to fracking.

Overall history of fracking, beginning with origins, discussion of general attitudes and motivations within the industries, describe general end goal that people are working on as stated by oil companies and another by environmentalist groups ples a transition into the timeline.Overall history of fracking, beginning with origins, discussion of general attitudes and motivations within the industries, describe general end goal that people are working on as stated by oil companies and another by environmentalist groups ples a transition into the timeline.

2004
Early Engineering of Whole Cell Biosensors

The first description of a whole-cell biosensor with environmental applications was described by researchers at the Swiss Federal Institute for Environmental Science and Technology. They engineered Pseudomonas putida bacteria to luminesce proportionately to aqueous naphthalene concentrations. However, these bacteria had complex biochemical pathways that were not well enough understood to be easily adjusted and refined. Furthermore, the cells were not sensitive enough to report the dilute naphthalene concentrations that fracking companies may be interested in reporting.



2005
Bush Signs Energy Policy Act

The Energy Policy Act of 2005 was passed to change US energy regulations, and restructuring tax incentives within the energy industries. Part of this bill included a provision that would exempt natural gas companies that use fracking from disclosing the chemicals involved that would normally be required of energy companies. While many drilling companies would benefit, it caused the started to grow skeptical about their intentions and the environmental impact of hydraulic fracturing.



2011
Founding of FracFocus Chemical Disclosure Agency

According to their website, FracFocus Chemical Disclosure is a government run agency that was the first to provide public access to information on fracking and the chemicals used near homes. Naphthalene was reported as the most common, as it was found in more than 30% of all contamination sites. Recent legislation has required companies to register the chemicals they use on FracFocus, and it coordinates with reporting companies to keep updated records of contaminant history across the United States. The foundation of the site and coordinating legislation provided transparency, but few companies have been held accountable for data published through FracFocus.



2012
OptiEnz Sensors Begin Use

Since the government had loose regulations on fracking contamination and people grew more concerned about the now-reported chemicals being used, the private sector’s demand for chemical sensors increased. OptiEnz is a leading biosensor development company that was founded by a professor at Colorado State University. Their product uses extracted enzymes and fiber optics to sense real-time pollution directly in the water source, preventing the need for pretreatment and skilled labor that has been previously required.



2013
Peking iGEM Team Improves Aromatic Transcriptional Regulators

Based on information from their wiki, the 2013 iGEM team from Peking developed many parts to detect aromatic compounds. Among those produced were the NahR transcriptional regulator combined with a salicylate promoter and an sfGFP gene that can respond to naphthalene at concentrations within an order of magnitude of the EPA’s maximum of 20 µg/L. This part was thoroughly characterized and available in the distribution kit, so it quickly became the goal of the CU 2015 team to improve its use to make the system more sensitive, easier to use, and ultimately more practical for industrial application.



2014
First Settlement of a Personal Injust Lawsuit Against a Fracking Company

Description of event with 8 components. 1. Motivation behind the action. 2. Who did it and why was it them. 3. What was accomplished. 4. Why was it accomplished. 5. What did it do that was so novel. 6. Who benefitted the most. 7. what questions did it leave unanswered. 8. Quote from some source. I’m in the process of filling out all these in a separate word document. each of the paragraphs will be approcimately 7 lines in length so this is just filler text so the formatting is easy later.



2015
Network Installation of Permenant Fracking Sensors Proposed at AMS Conference

Description of event with 8 components. 1. Motivation behind the action. 2. Who did it and why was it them. 3. What was accomplished. 4. Why was it accomplished. 5. What did it do that was so novel. 6. Who benefitted the most. 7. what questions did it leave unanswered. 8. Quote from some source. I’m in the process of filling out all these in a separate word document. each of the paragraphs will be approcimately 7 lines in length so this is just filler text so the formatting is easy later.



Description of event with 8 components. 1. Motivation behind the action. 2. Who did it and why was it them. 3. What was accomplished. 4. Why was it accomplished. 5. What did it do that was so novel. 6.

Team:CU-Boulder - 2015.igem.org