Team:CU Boulder/Composite Part


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Composite Parts



BBa_K1718001:

RFP under arabinose inducible pBad

This part has a Red Fluorescent Protein (RFP) downstream of the pBad promoter. Expression is stimulated by L-arabinose. We used this part to test the leakiness of this promoter.



BBa_K1718003:

RFP under the LuxPr promoter

In the presence of AHL, the LuxR transcription factor will bind AHL and activate the LuxPr promoter. RFP is downstream and will be expressed.



BBa_K1718004:

LuxI and GFP

LuxI leads to the production of AHL which, when in the presence of AHL, activates the LuxPr promoter. Downstream of the LuxI coding region is GFP, a reporter



BBa_K1718006:

Genetic switch for Bxb1 with LuxI and GFP

This part consists of the genetic switch described in BBa_K1718002 and the LuxI and GFP coding regions described in BBa_K1718004

Team:CU-Boulder - 2015.igem.org