Revision as of 23:16, 17 September 2015

"What I cannot create I do not understand."
- Richard Feynmann

Parameters

Name	Value	Description	References/Estimation
\(K_{d,\text{LuxRAHL}}\)	100 nM	Dissociation constant between luxR and AHL	Weber, 2013
\(\text{LuxR}_\text{tot}\)	0.0025 μM	Total concentration of LuxR	estimated
\(a_\mathrm{LuxI}\)	1 μM.min^-1	Maximal production rate of LuxI	Basu, 2005
\(a_\mathrm{LuxI,ribo}\)	0.1 μM.min^-1	Maximal production rate of LuxI	ETHZ 2014
\(k_\mathrm{leaky}\)	0.0375 μM^-1	Coefficient for leakiness dependency on LuxR concentration of P_LuxR promoter	ETHZ 2013
\(K_\mathrm{a,LuxRAHL}\)	9.89 nM	Activation coefficient of LuxRAHL	Estimated from our own data
\(K_\mathrm{LuxRAHL,ribo}\)	285 nM	Activation coefficient of LuxRAHL in case of a riboregulated LuxR responsive promoter	ETHZ 2014
\(L_\mathrm{lux,ribo}\)	0.01463 nM.min^-1	Leakiness after using riboswitch for P_lux	ETHZ 2014
\(n_\mathrm{lux}\)	1.7	Hill coefficient for LuxRAHL activation	ETHZ 2014
\(d_\mathrm{LuxI}\)	0.0167 min^-1	Degradation rate of LuxI	MIT 2010
\(a_\mathrm{AHL}\)	0.04 μM.min^-1	Production rate of AHL	Weber, 2013
\(d_\mathrm{AHL}\)	0.01 min^-1	Degradation rate of AHL	Basu, 2005
\(v_\mathrm{AiiA}\)	\(k_\mathrm{cat} \cdot C_\mathrm{AiiA} \)	Maximal conversion rate of AiiA
\(k_\mathrm{cat}\)	1.63 10³min^-1	Turnover number of AiiA	Wang, 2004
\(C_\mathrm{AiiA}\)	varied	Concentration of AiiA
\(K_\mathrm{M,AiiA}\)	2.95 10³ μM	Half-maximal rate substrate concentration of AiiA	Wang, 2004
\(a_\mathrm{GFP}\)	2 μM.min^-1	Maximal production rate of GFP	Basu, 2005
\(d_\mathrm{GFP}\)	0.01 min^-1	Degradation rate of GFP	estimated from doubling time of E. coli

Name	Value	Description	References/Estimation
\(N_{d}\)	150	Number of E. coli in the doughnut	Maximal number of E. coli that would fit on the surface
\(N_{b,max}\)	12798	Maximum number of E. coli in the bulk	Considering the maximal OD is 2
\(V_{cell,d}\)	6 μm³	Volume around an E. coli in the doughnut	estimated
\(V_{cell,b,worst}\)	78 μm³	Volume around an E. coli in the bulk	Worst case, estimated from \(N_{b,max}\)
\(V_{cell,b,norm}\)	1000 μm³	Volume around an E. coli in the bulk	Normal case

Name	Description	Minimum Value	Maximum Value	References/Estimation
\(D_\text{AHL,agar}\)	Diffusion coefficient of AHL in agar	\(3.0\times 10^{-10} m^2/s\)	\(4.41\times 10^{-10} m^2/s\)	Trovato, 2014 Fatin-Rouge, 2004

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