Difference between revisions of "Team:SZU China/Notebook"

Revision as of 03:58, 18 September 2015

• April 2nd - April 23rd

  To discuss the decision of team project: CANCER SNIPER BLADDER

• April 28th - June 20th

  Finished the project system in shuttle vector

• June 26th- June 26th

  Transfected the two plasmid system in 5637 and T24 bladder cancer cells,and got a good result in Rluc(with amber mutation). Moreover,we got the control experiments data in human bladder fiber epithelial normal cells(HFC).

• June 30th- June 30th

  Improved the original BioBrick TERT (BBa_K1722001).

• July 10th- July 25th

  In order to increase the expression of the system,we remoulded the system in three palsmid.

• July 12th- July 12th

  We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.

• July 18th- July 18th

  Began to distribute questionnaires Onlinehttp://www.sojump.com/jq/5569949.aspx

• July 28th- July 28th

  Transfected the three plasmid system in the T24 and 5637 bladder cancer cells,but its result was not so good.Therefore,we continued to use the two plasmid system.

• July 30th- July 30th

  Propaganda synthetic biology and iGEM to all students at the Cultural Festival of the Collage of Life Science.

• August 7th- August 15th

  Finished some BioBricks:shTERT+GFP Composite(BBa_K1722009), TERT part(BBa_K1722002), shTERT part(BBa_K1722001),hUPII part(BBa_K1722000) and Rlu part(BBa_K1722005).

• August 16th- August 25th

  Added GFP(with amber mutation) to the two plasmid system, and transfected the system in 5637 and T24 bladder cancer cells.

• August 18th- August 26th

  Finished some BioBricks:SV40(en) part(BBa_K1722006), SV40+Rlu Composite(BBa_K1722013), tRNA part(BBa_K1722004), SV40(en)+Rlu Composite(BBa_K1722012)

• August 19th- August 19th

  LZU-iGEM and SZU-iGEM made a whole day of cooperative communication.

• August 23th- August 23th

  The GFP gene transfection test was successful, and it proved that THE BLADDER CANCER SNIPER SYSTERM was functional.

• August 26th- September 10th

  Finished the rest of BioBricks:ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite（BBa_K1722010），AckRS part(BBa_K1722003),hUPll+AckRS Composite(BBa_K1722007)

• September 2th- September 13th

  Finished the rest of BioBricks:hUPll+AckRS Composite(BBa_K1722007),AckRS part(BBa_K1722003),ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite(BBa_K1722010)

• September 3th- September 15th

  Finished collecting BioBricks data, and edited in the wiki.https://2015.igem.org/Team:SZU_China/Parts

• September 5th- September 5th

  we visited the Shenzhen Cancer Rehabilitation Association,and made a thorough understanding about specific situation of cancer patients.

• September 8th- September 15th

  In order to prove the system's function and specificity, we made second GFP transfection test.The result indicated the system has a good function and specificity.

• September 11th- September 11th

  According to our experience, we convened cancer patients,and made a cancer patients communication,Which purpose is to give patients confidence and improve the difficult situation of group's treatment in china.