Team:SZU China/Notebook



Notebook




  • To discuss the decision of team project: CANCER SNIPER BLADDER

  • Finished the project system in shuttle vector

  • In order to verify the function of our UAA orthogonal system,We constructed the whole system into three plasmids,and then we transfected the three-plasmid-system in T24 bladder cancer cells.We got a good result in Rluc(with amber mutation) detection.

  • Improved the original BioBrick TERT(BBa_K1722001)

  • In order to verify the function of hTERT and hUPII,We constructed the whole system into two plasmids,and then we transfected the two-plasmid-system in 5637 bladder cancer cells.We got a good result in Luc(with amber mutation) detection.At the same time,we transfected the two-plasmid-system in HFC and Hela to build controll groups.

  • We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.

  • Began to distribute questionnaires Online2015SZU-iGEM Questionnaire

  • We distributed our paper questionnaiers to many places.For instence,People's Hospital of Guangdong Province,Shenzhen Hospital and Genitourinary Center of Peking University Hospital.Via our offorts,we got many important data for our project.

  • Propaganda synthetic biology and iGEM to all students at the Cultural Festival of Collage of Life Sciences Shenzhen University.

  • Finished some BioBricks:shTERT+GFP Composite(BBa_K1722009), TERT part(BBa_K1722002), shTERT part(BBa_K1722001),hUPII part(BBa_K1722000) and Rlu part(BBa_K1722005).

  • Added GFP(with amber mutation) to the two plasmid system, and transfected the system in 5637 and T24 bladder cancer cells.

  • Finished some BioBricks:SV40(en) part(BBa_K1722006), SV40+Rlu Composite(BBa_K1722013), tRNA part(BBa_K1722004), SV40(en)+Rlu Composite(BBa_K1722012)

  • LZU-iGEM and SZU-iGEM made a whole day of cooperative communication.

  • The GFP gene transfection test was successful, and it proved that THE BLADDER CANCER SNIPER SYSTERM was functional.

  • Finished the rest of BioBricks:ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite(BBa_K1722010），hUPll+AckRS Composite(BBa_K1722007)

  • Prepared wiki,continued the system verification experiment

  • Finished collecting BioBricks data, and edited in the wiki.https://2015.igem.org/Team:SZU_China/Basic_parts

  • we visited the Shenzhen Cancer Rehabilitation Association,and made a thorough understanding about specific situation of cancer patients.

  • In order to prove the system's function and specificity, we made second GFP transfection test.The result indicated the system has a good function and specificity.

  • According to our experience, we convened cancer patients,and made a cancer patients communication,Which purpose is to give patients confidence and improve the difficult situation of group's treatment in china.

  • These are our note PDFs for download:
    2015 SZU igem note protocol 1.0
    2015 SZU igem note protocol 2.0
    2015 SZU igem note protocol 3.0