Difference between revisions of "Team:SZU China/Practices"

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<div class="text-title">
 
<div class="text-title">
 
  <div class="texts">
 
  <div class="texts">
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        <p id="heading">Human practice</p>
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<br>
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        <p id="brief">Combine Biological Unnatural Amino Acid（UAA）Orthogonal
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<br>
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            <br>genectical system with logic "AND" gate gene circuit
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            <br>to detect bladder cancer cells precisely</p>
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    <p id="heading"><small>Notebook</small></p>
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     </div>
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     </div></br>
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        <div id="boody" data-spy="scroll" data-target=".navbar" data-offset="50">
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            <nav class="navbar navbar-default" data-spy="affix" data-offset-top="319">
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<ul class="cbp_tmtimeline" style="margin: 4em 3em 0 9em;">
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                    <button type="button" class="navbar-toggle" data-toggle="collapse" data-target="#myNavbar">
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<li>
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                        <span class="icon-bar"></span>
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<time class="cbp_tmtime" datetime="2013-04-10 18:30"><span>April 2nd -</span> <span> April 23rd</span></time>
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                        <span class="icon-bar"></span>
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<div class="cbp_tmicon cbp_tmicon-phone"></div>
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<p>To discuss the decision of team project: CANCER SNIPER BLADDER</p>
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</div>
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                <div>
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</li>
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                    <div id= "center_bar" class="collapse navbar-collapse" id="myNavbar">
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<li>
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                            <ul class="nav navbar-nav">
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<time class="cbp_tmtime" datetime="2013-04-11T12:04"><span>April 28th -</span> <span> June 20th</span></time>
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                                <li><a href="#section1">2011 &nbsp;&nbsp;&nbsp;&nbsp;></a>
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<div class="cbp_tmicon cbp_tmicon-screen"></div>
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                                </li>
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<div class="cbp_tmlabel">
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                                <li><a href="#section2">2012 &nbsp;&nbsp;&nbsp;&nbsp;></a>
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                                </li>
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<p>Finished the project system in shuttle vector</p>
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                                <li><a href="#section3">2013 &nbsp;&nbsp;&nbsp;&nbsp;></a>
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</div>
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                                </li>
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</li>
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                                <li><a href="#section41">2014 &nbsp;&nbsp;&nbsp;&nbsp;></a>
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<li>
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                                </li>
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<time class="cbp_tmtime" datetime="2013-04-13 05:36"><span>June 26th-</span> <span> June 26th</span></time>
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                                <li><a href="#section42">2015 &nbsp;&nbsp;&nbsp;</a>
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<div class="cbp_tmicon cbp_tmicon-mail"></div>
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                                </li>
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<div class="cbp_tmlabel">
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<p>Transfected the two plasmid system in 5637 and T24 bladder cancer cells,and got a good result in Rluc(with amber mutation). Moreover,we got the control experiments data in human bladder fiber epithelial normal cells(HFC).</p>
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                            </ul>
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                        </div>
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</li>
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                </div>
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<li>
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<time class="cbp_tmtime" datetime="2013-04-15 13:15"><span>June 30th-</span> <span> June 30th</span></time>
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            </nav>
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<div class="cbp_tmicon cbp_tmicon-phone"></div>
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            <div id="section1" class="container-fluid">
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<div class="cbp_tmlabel">
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                <h1>Section 1</h1>
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                 <p>Improved the original BioBrick TERT (BBa_K1722001). </p>
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</div>
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                <p>Try to scroll this section and look at the navigation bar while scrolling! Try ection and look at the navigation bar while scrolling! Try ection and look at the navigation bar while scrolling! Try ection and look at the navigion and look at the navigation bar while scrolling! Try ection and look at the navigation bar while scrolling! Try ection and look at the navigation bar while scrolling! Try ection and look at the navigation bar while scrolling! Try ection and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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</li>
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                <p>Try to scroll this section and look at the nato scroll this section and look at the nato scroll this section and look at the nato scroll this section and look at the nato scroll this section and look at the nato scroll this sectioscroll this section and look at the nato scroll this section and look at the nato scroll this sectioscroll this section and look at the nato scroll this section and look at the nato scroll this sectioscroll this section and look at the nato scroll this section and look at the nato scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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<li>
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            </div>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>July 10th-</span> <span>July 25th</span></time>
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            <div id="section2" class="container-fluid">
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<div class="cbp_tmicon cbp_tmicon-earth"></div>
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                <h1>Section 2</h1>
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<div class="cbp_tmlabel">
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<p>In order to increase the expression of the system,we remoulded the system in three palsmid.</p>
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                <p>Try to scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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</div>
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                <p>Try to scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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</li>
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            </div>
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<li>
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            <div id="section3" class="container-fluid">
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>July 12th-</span> <span>July 12th</span></time>
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                <h1>Section 3</h1>
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<div class="cbp_tmicon cbp_tmicon-earth"></div>
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<div class="cbp_tmlabel">
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                <p>Try to scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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<p>We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.</p>
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                <p>Try to scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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</div>
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            </div>
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</li>
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            <div id="section41" class="container-fluid">
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<li>
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                <h1>Section 4 Submenu 1</h1>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>July 18th-</span> <span>July 18th</span></time>
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+
<div class="cbp_tmicon cbp_tmicon-earth"></div>
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                <p>Try to scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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<div class="cbp_tmlabel">
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                <p>Try to scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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<p>Began to distribute questionnaires Online<a href:"http://www.sojump.com/jq/5569949.aspx">http://www.sojump.com/jq/5569949.aspx</a></p>
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            </div>
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</div>
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            <div id="section42" class="container-fluid">
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</li>
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                <h1>Section 4 Submenu 2</h1>
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<li>
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<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>July 28th-</span> <span>July 28th</span></time>
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                <p>Try to scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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<div class="cbp_tmicon cbp_tmicon-earth"></div>
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                <p>Try to scroll this section and look at the navigation bar while scrolling! Try to scroll this section and look at the navigation bar while scrolling!</p>
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<div class="cbp_tmlabel">
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            </div>
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<p>Transfected the three plasmid system in the T24  and 5637 bladder cancer cells,but its result was not so good.Therefore,we continued to use the two plasmid system. </p>
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        </div>
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</div>
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        <div class="footer">
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</li>
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            <p>Team SZU-China</p>
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<li>
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        </div>
+
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>July 30th-</span> <span>July 30th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>Propaganda synthetic biology and iGEM to all students at the Cultural Festival of the Collage of Life Science.</p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>August 7th-</span> <span>August 15th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>Finished some BioBricks:shTERT+GFP Composite(BBa_K1722009), TERT part(BBa_K1722002), shTERT part(BBa_K1722001),hUPII part(BBa_K1722000) and Rlu part(BBa_K1722005).</p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>August 16th-</span> <span>August 25th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>Added GFP(with amber mutation) to the two plasmid system, and transfected the system in 5637 and T24 bladder cancer cells.</p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>August 18th-</span> <span> August 26th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>Finished some BioBricks:SV40(en) part(BBa_K1722006), SV40+Rlu Composite(BBa_K1722013), tRNA part(BBa_K1722004), SV40(en)+Rlu Composite(BBa_K1722012)</p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>August 19th-</span> <span>August 19th </span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>LZU-iGEM and SZU-iGEM made a whole day of cooperative communication.
 +
 
 +
</p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>August 23th-</span> <span>August 23th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>The GFP gene transfection test was successful, and it proved that THE BLADDER CANCER SNIPER SYSTERM was functional.</p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>August 26th-</span> <span> September 10th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>Finished the rest of BioBricks:ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite（BBa_K1722010），AckRS part(BBa_K1722003),hUPll+AckRS Composite(BBa_K1722007)
 +
 
 +
</p>
 +
</div>
 +
</li>
 +
<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>September 2th-</span> <span> September 13th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>Finished the rest of BioBricks:hUPll+AckRS Composite(BBa_K1722007),AckRS part(BBa_K1722003),ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite(BBa_K1722010) </p>
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</div>
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</li>
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<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>September 3th-</span> <span> September 15th</span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>Finished collecting BioBricks data, and edited in the wiki.<a href=" https://2015.igem.org/Team:SZU_China/Parts">https://2015.igem.org/Team:SZU_China/Parts</a></p>
 +
</div>
 +
</li>
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<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>September 5th-</span> <span>September 5th </span></time>
 +
<div class="cbp_tmicon cbp_tmicon-earth"></div>
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<div class="cbp_tmlabel">
 +
<p>we visited the Shenzhen Cancer Rehabilitation Association,and made a thorough understanding about specific situation of cancer patients.</p>
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</div>
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</li>
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<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>September 8th-</span> <span> September 15th</span></time>
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<div class="cbp_tmicon cbp_tmicon-earth"></div>
 +
<div class="cbp_tmlabel">
 +
<p>In order to prove the system's function and specificity, we made second GFP transfection test.The result indicated the system has a good function and specificity.
 +
 
 +
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 +
</div>
 +
</li>
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<li>
 +
<time class="cbp_tmtime" datetime="2013-04-16 21:30"><span>September 11th-</span> <span>September 11th  </span></time>
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<div class="cbp_tmicon cbp_tmicon-earth"></div>
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Revision as of 07:25, 18 September 2015



Notebook




  • To discuss the decision of team project: CANCER SNIPER BLADDER

  • Finished the project system in shuttle vector

  • Transfected the two plasmid system in 5637 and T24 bladder cancer cells,and got a good result in Rluc(with amber mutation). Moreover,we got the control experiments data in human bladder fiber epithelial normal cells(HFC).

  • Improved the original BioBrick TERT (BBa_K1722001).

  • In order to increase the expression of the system,we remoulded the system in three palsmid.

  • We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.

  • Began to distribute questionnaires Onlinehttp://www.sojump.com/jq/5569949.aspx

  • Transfected the three plasmid system in the T24 and 5637 bladder cancer cells,but its result was not so good.Therefore,we continued to use the two plasmid system.

  • Propaganda synthetic biology and iGEM to all students at the Cultural Festival of the Collage of Life Science.

  • Finished some BioBricks:shTERT+GFP Composite(BBa_K1722009), TERT part(BBa_K1722002), shTERT part(BBa_K1722001),hUPII part(BBa_K1722000) and Rlu part(BBa_K1722005).

  • Added GFP(with amber mutation) to the two plasmid system, and transfected the system in 5637 and T24 bladder cancer cells.

  • Finished some BioBricks:SV40(en) part(BBa_K1722006), SV40+Rlu Composite(BBa_K1722013), tRNA part(BBa_K1722004), SV40(en)+Rlu Composite(BBa_K1722012)

  • LZU-iGEM and SZU-iGEM made a whole day of cooperative communication.

  • The GFP gene transfection test was successful, and it proved that THE BLADDER CANCER SNIPER SYSTERM was functional.

  • Finished the rest of BioBricks:ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite（BBa_K1722010），AckRS part(BBa_K1722003),hUPll+AckRS Composite(BBa_K1722007)

  • Finished the rest of BioBricks:hUPll+AckRS Composite(BBa_K1722007),AckRS part(BBa_K1722003),ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite(BBa_K1722010)

  • Finished collecting BioBricks data, and edited in the wiki.https://2015.igem.org/Team:SZU_China/Parts

  • we visited the Shenzhen Cancer Rehabilitation Association,and made a thorough understanding about specific situation of cancer patients.

  • In order to prove the system's function and specificity, we made second GFP transfection test.The result indicated the system has a good function and specificity.

  • According to our experience, we convened cancer patients,and made a cancer patients communication,Which purpose is to give patients confidence and improve the difficult situation of group's treatment in china.