Difference between revisions of "Team:San Andres/Modeling"

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<h2> Modeling</h2>
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<h4>Note</h4>
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Model award</a>, you must fill out this page.</p>
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<img src="https://static.igem.org/mediawiki/2015/0/09/Firma_800.jpg"
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<table class="gwd-table-nb4m editable" width="100%">
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      <a href="https://2015.igem.org/Team:San_Andres"
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style="color: rgb(0, 0, 0);">Home </a> </td>
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      <a href="https://2015.igem.org/Team:San_Andres/Team"
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style="color: rgb(0, 0, 0);"> Team </a> </td>
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      <a href="https://2015.igem.org/Team:San_Andres/Description"
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style="color: rgb(0, 0, 0);"> Project </a>
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      <a href="https://2015.igem.org/Team:San_Andres/Notebook"
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style="color: rgb(0, 0, 0);"> Statistics</a>
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      </td>
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      <a href="https://2015.igem.org/Team:San_Andres/Experiments"
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style="color: rgb(0, 0, 0);"> Enzymes</a>
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      <a href="https://2015.igem.org/Team:San_Andres/Parts"
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style="color: rgb(0, 0, 0);"> Parts</a>
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      <a href="https://2015.igem.org/Team:San_Andres/Notebook"
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style="color: rgb(0, 0, 0);"> Metodology</a>
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      <a href="https://2015.igem.org/Team:San_Andres/Results"
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style="color: rgb(0, 0, 0);"> Results </a>
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      <a href="https://2015.igem.org/Team:San_Andres/Collaborations"
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style="color: rgb(0, 0, 0);"> Future Projections </a>
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      <a href="https://igem.org/Team_List?year=2015"><img
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src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"
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width="55"></a></td>
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<h1>Metodology&nbsp;</h1>
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<big>The methodology consists of cutting and pasting, with
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ligases and restriction enzymes as a "waterfall", for final assembly
 +
that will produce both proteins. The motive of this assembly is due to
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the fact that the promoter, the RBS and the terminator were too small,
 +
and if you take out them from their original plasmids, these are lost,
 +
so, simply sticking and peeling it off one by one, we arrived to a
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linear Assembly in the three corresponding plasmids (promoter, RBS and
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terminator).</big><br>
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<br>
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<div style="text-align: center;"><img
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alt="File:Modelo cascada.jpg"
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src="https://static.igem.org/mediawiki/2015/thumb/1/1c/Modelo_cascada.jpg/800px-Modelo_cascada.jpg"
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height="539" width="800"><br>
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<div style="text-align: left;"><big>At this time we
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are innovating ideas to add a circuit that will allow us in the future
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to obtain a method of detecting and quantifying the presence of gluten,
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which can also be checked by a commercial kit.</big><br>
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<br>
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<div style="text-align: center;"><img
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alt="File:Kit gluten.jpg"
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src="https://static.igem.org/mediawiki/2015/0/09/Kit_gluten.jpg"
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height="513" width="593"></div>
 
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<p>Mathematical models and computer simulations provide a great way to describe the function and operation of BioBrick Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. Designing and simulating can be iterated many times in a computer before moving to the lab. This award is for teams who build a model of their system and use it to inform system design or simulate expected behavior in conjunction with experiments in the wetlab.</p>
 
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<p>
 
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Here are a few examples from previous teams:
 
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</p>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:ETH_Zurich/modeling/overview">ETH Zurich 2014</a></li>
 
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<li><a href="https://2014.igem.org/Team:Waterloo/Math_Book">Waterloo 2014</a></li>
 
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<br>
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<big></big>
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Revision as of 20:18, 5 July 2015

wiki 2

Home Team Project Statistics Enzymes Parts Metodology Results Future Projections

Metodology 

The methodology consists of cutting and pasting, with ligases and restriction enzymes as a "waterfall", for final assembly that will produce both proteins. The motive of this assembly is due to the fact that the promoter, the RBS and the terminator were too small, and if you take out them from their original plasmids, these are lost, so, simply sticking and peeling it off one by one, we arrived to a linear Assembly in the three corresponding plasmids (promoter, RBS and terminator).

File:Modelo cascada.jpg
At this time we are innovating ideas to add a circuit that will allow us in the future to obtain a method of detecting and quantifying the presence of gluten, which can also be checked by a commercial kit.

File:Kit gluten.jpg