The sequencing confirmed the accuracy of the devices. At first a contig (black) was assebled from the sequencing data. The forward sequencing is shown in blue and the reverse sequencing in rose. To see the alignment of the respective contig and the device sequence (green) click the image.

Sequence assembly for device 1:

Sequence assembly for device 2:

Sequence assembly for device 3:

Moreover, after transformation of the devices 1-3 we could already see some fluorescence on the plates (Fig. 2). To confirm the GFP fluorescence we took a photo with a smartphone and our filter combination for GFP detection.

The fluorescence was measured with a plate reader (Tecan Reader). The excitation wavelength was 475 nm and the emission wavelength 509 nm. As expected there was almost no fluorescence for the negative controls and about 14000 RFU for the positive control (Fig. 3). The highest fluorescence was measured for device 1 (47000 RFU) confirming the expectation as device 1 carries the strongest promoter.
Unexpectedly, one biological replicate of device 1 shows a much lower fluorescence (Fig. 3). A reason for that could be a dilution error. To review this possibility the dilutions were prepared again. However, the results were the same. At a second glance on the plates it could be determined that there are already colonies with different levels of staining although they were plated out from one colony.