Difference between revisions of "Team:UCSC/Parts"

Line 253: Line 253:
 
<!--First Block -->
 
<!--First Block -->
  
−
<div id="our_content" align="center">
+
<div id="our_content">
  
 
<h1> Parts</h2>
 
<h1> Parts</h2>
−
<p text-align: left>
+
<p>
 
>Natural halophilic protein linker 1
 
>Natural halophilic protein linker 1
 +
<br/>
 
CGGCGAGAGAACATGCTGTGGTTTCGAGTGCCC
 
CGGCGAGAGAACATGCTGTGGTTTCGAGTGCCC
−
 
+
<br/>
 
>Natural halophilic protein linker 2
 
>Natural halophilic protein linker 2
 +
<br/>
 
CGAGCCGAGAACATGCTGTGGCACAAACTTCCC
 
CGAGCCGAGAACATGCTGTGGCACAAACTTCCC
−
 
+
<br/>
 
>Engineered rigid halophilic protein linker
 
>Engineered rigid halophilic protein linker
 +
<br/>
 
GAAGCTGCTGCTAAAGAAGCTGCTGCTAAA
 
GAAGCTGCTGCTAAAGAAGCTGCTGCTAAA
−
 
+
<br/>
 
>Engineered flexible halophilic protein linker
 
>Engineered flexible halophilic protein linker
 +
<br/>
 
GGAGGAGGAGGAAGTGGAGGAGGAGGAAGT
 
GGAGGAGGAGGAAGTGGAGGAGGAGGAAGT
−
 
+
<br/>
 
>Codon Optimized Shewanella Sediminis Alcohol Dehydrogenase for expression in Haloferax Volcanii
 
>Codon Optimized Shewanella Sediminis Alcohol Dehydrogenase for expression in Haloferax Volcanii
 +
<br/>
 
ATGACGGTCACGAACGAGCAAGAGCTCGATCTGCTGGTCCAGCGAGTGGCCGATGCGCAG
 
ATGACGGTCACGAACGAGCAAGAGCTCGATCTGCTGGTCCAGCGAGTGGCCGATGCGCAG
 
GCCGAGTACGCCAATTTCTCGCAGGAGCAGGTGGACAAGATTTTCCGAGCGGCGGCGCTG
 
GCCGAGTACGCCAATTTCTCGCAGGAGCAGGTGGACAAGATTTTCCGAGCGGCGGCGCTG
Line 314: Line 319:
 
AATCCGCGCTACCCGCTGATTGAGGAACTGAAGCAAGTCCTCCTCGCGTCGTTCTACGGC
 
AATCCGCGCTACCCGCTGATTGAGGAACTGAAGCAAGTCCTCCTCGCGTCGTTCTACGGC
 
ACGGCCTATTCGGACAAGTGA
 
ACGGCCTATTCGGACAAGTGA
−
 
+
<br/>
 
>Codon optimized Zymomonas mobilis pyruvate decarboxylase for expression in Haloferax Volcanii
 
>Codon optimized Zymomonas mobilis pyruvate decarboxylase for expression in Haloferax Volcanii
 +
<br/>
 
ATGTCGTACACGGTCGGCACGTACCTCGCCGAGCGCCTCGTCCAGATCGGCCTCAAACAC
 
ATGTCGTACACGGTCGGCACGTACCTCGCCGAGCGCCTCGTCCAGATCGGCCTCAAACAC
 
CACTTCGCGGTGGCCGGAGATTACAACCTGGTCCTGCTCGACAACCTCCTCCTCAACAAA
 
CACTTCGCGGTGGCCGGAGATTACAACCTGGTCCTGCTCGACAACCTCCTCCTCAACAAA
Line 345: Line 351:
 
GCCGCGAAGACTGCACGGAAGAACTCGTCAAATGGGGCAAGCGCGTGGCCGCCGCCAATA
 
GCCGCGAAGACTGCACGGAAGAACTCGTCAAATGGGGCAAGCGCGTGGCCGCCGCCAATA
 
GCCGCAAACCGGTCAACAAGCTCCTCTGA
 
GCCGCAAACCGGTCAACAAGCTCCTCTGA
−
 
+
<br/>
 
>Codon Optimized Haloarcula Hispanica beta-glucosidase
 
>Codon Optimized Haloarcula Hispanica beta-glucosidase
 +
<br/>
 
ATGGCACATGACACGACTGACGACGGTATGCAGGCGTCTCGGCGGACATTTCTGAAAGCG
 
ATGGCACATGACACGACTGACGACGGTATGCAGGCGTCTCGGCGGACATTTCTGAAAGCG
 
ACCGGTGTGGCCACGGCAGCGGCCGCCACCGGCGCAGGTGTTGGGACCGCCGCGGCACAA
 
ACCGGTGTGGCCACGGCAGCGGCCGCCACCGGCGCAGGTGTTGGGACCGCCGCGGCACAA

Revision as of 22:48, 18 September 2015

Parts

>Natural halophilic protein linker 1
CGGCGAGAGAACATGCTGTGGTTTCGAGTGCCC
>Natural halophilic protein linker 2
CGAGCCGAGAACATGCTGTGGCACAAACTTCCC
>Engineered rigid halophilic protein linker
GAAGCTGCTGCTAAAGAAGCTGCTGCTAAA
>Engineered flexible halophilic protein linker
GGAGGAGGAGGAAGTGGAGGAGGAGGAAGT
>Codon Optimized Shewanella Sediminis Alcohol Dehydrogenase for expression in Haloferax Volcanii
ATGACGGTCACGAACGAGCAAGAGCTCGATCTGCTGGTCCAGCGAGTGGCCGATGCGCAG GCCGAGTACGCCAATTTCTCGCAGGAGCAGGTGGACAAGATTTTCCGAGCGGCGGCGCTG GCGGCCGCGGATGCCCGGATCAGCCTCGCGAAGATGGCGGCGAATGAAACGGGCATGGGC GTGATCGAGGACAAGGTCATCAAGAATCACTTCGCCTCGGAGTACATTTACAACAAGTAC AAGGATGAGAAAACCTGTGGCATTCTGGACGAGGATCCCACGTTCGGCACGATCACCATC GCGGAGCCGGTCGGTATCATTTGCGGGATCGTCCCGACGACGAACCCCACCTCCACGGCG ATCTTTAAGGCCCTGATTTCGCTCAAGACCCGCAACGGCATCATCTTCTCGCCGCACCCC CGAGCCAAGGAGTCCACCACGACGGCGGCCCGGCTCGTCCTGAATGCCGCGATCGAAGCG GGTGCGCCCAAGGATATTATCGGGTGGATCGATGAGCCGTCGGTGGCCCTGTCGAACCAG CTCATGACGCATGATAAGATTAACCTCATTCTCGCCACCGGCGGTCCCGGTATGGTGAAG GCCGCCTACTCGTCGGGGAAGCCGGCCATTGGGGTGGGTGCCGGGAACACCCCCATCGTC ATTGACGAAACCGCGGACATCAAGCGCGCGGTGTCGTCCATCCTCATGTCCAAGACCTTT GACAACGGGGTGGTGTGCGCGTCGGAGCAAGCCGTGGTCATTGTCGACGAAATTTACGAT ACGGTCAAGGAGCGGTTTGCCACGCACGGGGGCTACATTCTCAGCAAAGACGAGACCGCC GCCATGCAAAATGTCATTCTGAAGAATGGCGGGCTCAACGCGGATATCGTCGGTCAGAGC GCCGTGACCATTGCGGCGATGGCCGACATTGAGGTCCCCTCGTGGACGAAGGTCCTGATT GGTGAGGTCGAGGATGTCTCGGAGGCGGAGGCCTTTGCGCATGAGAAGCTCAGCCCGCTG CTGGGCATGTACCGCGTGAAGGACTTTGAAGCCGCCATGGACAAAGCCGAAGCCCTGGTC ACCCTCGGGGGTATCGGTCACACGAGCGGTCTGTACACGAACCAAGACACGCAGACGGAA CGCGTGATGGCGTTTGGCTTCCGCATGAAGACGGCGCGCATCCTGATCAACACGCCGGCG TCCCAAGGTGGTATTGGCGACCTGTATAACTTTAAGCTGGCCCCCTCGCTCACGCTGGGC TGCGGCAGCTGGGGTGGGAATTCGATTAGCGAGAACGTCGGCCCGTCGCACCTCATCAAT AAGAAAATGGTCGCGAAACGCGCGGAGAACATGCTCTGGCACAAGCTCCCGTCGTCGATC TACTTCCGACGGGGGTCGCTCCCGATTGCCCTCGAAGAACTCAGCGATAAAAAGCGAGCG CTGATCGTCACGGATAAGTACCTCTTTAATAACGGCTATTGCGATGAGACCATCAAAATT CTCAAGTCGCAGGGGCTCGAAACGGAGATCTTCTACGAAGTGGAAGCGGACCCGACGCTC GCGATTGTCACGCAGGGCGCGAAGATGGCCCAATCGTTCCAGCCGGATGTGATCATTGCC CTGGGTGGGGGCAGCCCGATGGATGCCGCCAAGATTATTTGGGTCATGTACGAGCACCCG GACGTCGATTTTGCGGACCTCGCCCTCCGGTTTATGGACATTCGCAAGCGCATCTACAAG TTCCCGAAACTGGGGGTCAAAGCGCAGATGGTGGCCATCCCGACCACGTCGGGCACCGGC AGCGAGGTGACGCCGTTCGCGGTCGTCACCGATGAAAAGACGGGTATGAAGTACCCCATT GCCGATTATCAACTGACGCCCAACATGGCGATCGTGGATCCGAACCTGGTGATGGATATG CCGAAGTCGCTGACCGCCTTCGGGGGGATTGACGCGGTGACCCACGCGCTCGAGGCCTAC GTCTCGGTCATGGCCAACGAATACTCGGACGGCCAGGCGCTCCAAGCGCTGGATCTCCTC TTCAAGCACCTCCCGGACAGCTACAACCACGGTGCCGCGGCGCCCATCGCCCGGGAAAAG GTCCACAATGGCGCGACGATTGCGGGGATCGCGTTCGCCAACGCCTTCCTCGGGATTTGT CACAGCATGGCCCATAAGCTGGGGGCCGAATTTCACCTGGCCCACGGGCTCGCCAATGCC CTGCTCATTAACAACGTCATCCGGTTCAACGCGACGGACATGCCGACGAAGCAGGCGGCG TTTTCGCAGTACGATCGACCCAAGGCGCTGTGCCGCTACGCGAAAATCGCCGAATATCTG AACCTGGGCGGTACGACGGATGAGGAGAAGGTGGAGGCCCTCATTGAAGAAGTCGACCAG CTGAAAAAAACCATTGGTATTCCGGCGAGCATTCGAGAGGCGGGTGTCAACGAGGCCGAC TTTCTGGCCAAACTCGATGAACTCGCCGAGGACGCGTTTGACGATCAATGTACGGGCGCC AATCCGCGCTACCCGCTGATTGAGGAACTGAAGCAAGTCCTCCTCGCGTCGTTCTACGGC ACGGCCTATTCGGACAAGTGA
>Codon optimized Zymomonas mobilis pyruvate decarboxylase for expression in Haloferax Volcanii
ATGTCGTACACGGTCGGCACGTACCTCGCCGAGCGCCTCGTCCAGATCGGCCTCAAACAC CACTTCGCGGTGGCCGGAGATTACAACCTGGTCCTGCTCGACAACCTCCTCCTCAACAAA AACATGGAGCAGGTCTACTGCTGCAACGAACTGAATTGCGGTTTTTCCGCCGAGGGCTAC GCGCGAGCGAAAGGTGCCGCGGCGGCGGTCGTGACGTACTCGGTCGGGGCCCTGTCGGCC TTCGACGCGATCGGAGGAGCGTATGCGGAGAACCTCCCCGTCATCCTGATCTCGGGTGCG CCGAATAACAACGATCATGCCGCCGGTCACGTCCTCCACCACGCCCTGGGCAAGACGGAT TACCACTACCAGCTCGAAATGGCGAAGAACATTACCGCCGCCGCGGAAGCCATCTACACG CCCGAAGAGGCGCCGGCCAAGATTGATCATGTCATTAAGACGGCCCTGCGAGAGAAAAAG CCGGTCTACCTCGAGATCGCCTGTAACATTGCCTCGATGCCGTGTGCCGCCCCCGGTCCC GCGTCGGCGCTCTTCAATGATGAAGCCTCGGACGAAGCGTCGCTCAATGCGGCCGTCGAG GAGACGCTGAAATTCATCGCCAACCGCGACAAGGTGGCGGTCCTCGTCGGCTCGAAGCTG CGAGCCGCGGGTGCCGAGGAAGCCGCCGTCAAGTTCGCGGATGCGCTGGGGGGAGCGGTC GCCACGATGGCGGCCGCGAAAAGCTTCTTCCCGGAAGAGAACCCGCACTATATCGGTACG TCGTGGGGTGAGGTCTCCTATCCGGGTGTGGAGAAGACGATGAAGGAGGCGGACGCGGTC ATCGCGCGCTCGCCCCGGTGTTCAACGACTACAGCACCACGGGCTGGACGGACATCCCGG ACCCGAAAAAACTCGTGCTCGCGGAGCCGCGATCCGTCGTCGTGAATGGCATTCGCTTCC CGTCGGTCCACCTCAAGGACTACCTCACGCGCCTGGCCCAAAAGGTCTCGAAGAAAACCG GAGCCCTGGACTTCTTCAAGTCGCTGAATGCCGGTGAACTCAAGAAAGCGGCGCCCGCGG ATCCCTCCGCCCCGCTCGTGAACGCGGAAATCGCCCGCCAGGTCGAAGCGCTCCTGACGC CGAACACGACGGTGATTGCCGAGACGGGCGACTCGTGGTTCAATGCCCAACGCATGAAGC TCCCGAACGGAGCGCGGGTCGAATACGAGATGCAGTGGGGCCACATCGGCTGGTCGGTGC CGGCCGCCTTCGGCTATGCGGTGGGAGCCCCGGAACGGCGAAATATTCTCATGGTCGGCG ACGGCTCGTTTCAGCTCACGGCGCAGGAGGTCGCCCAGATGGTCCGACTGAAACTCCCGG TCATTATCTTCCTCATCAACAACTACGGCTACACGATCGAGGTCATGATCCATGATGGGC CCTACAACAACATCAAAAACTGGGACTACGCGGGCCTGATGGAAGTCTTCAACGGCAATG GCGGATACGACAGCGGTGCGGGCAAGGGTCTCAAAGCGAAAACGGGTGGCGAACTGGCCG AGGCCATCAAGGTCGCGCTGGCGAATACGGATGGCCCGACGCTCATCGAGTGCTTCATTG GCCGCGAAGACTGCACGGAAGAACTCGTCAAATGGGGCAAGCGCGTGGCCGCCGCCAATA GCCGCAAACCGGTCAACAAGCTCCTCTGA
>Codon Optimized Haloarcula Hispanica beta-glucosidase
ATGGCACATGACACGACTGACGACGGTATGCAGGCGTCTCGGCGGACATTTCTGAAAGCG ACCGGTGTGGCCACGGCAGCGGCCGCCACCGGCGCAGGTGTTGGGACCGCCGCGGCACAA CCGGACGTCGACAGTCTCATTGGCGACCTCACCCTCGAGCAGAAAGCGGCCCAGATGACG CAGGTCGCGATCTCGTCGTTCGAAGCCGAACCGGAAGAGTCGAATGTGCCGGATTCGTTT GGGGTCGACACGGTCGGCGAGTACTTCAGCGAGCTGGGCATCGGGTCGATCCTGTCGGGT GGCGCGGAACCGCCGTCGTTTGACGCGAACGCGGTCGTCCAGGGGATCAACGCCCTGCAG CAATACAACCTCGACAACGCGGACCACGACATCCCGTTCCTCTACGGGGTGGATGCGACG CACGGCAACGGCCTCCTGGAAGGGGCCACCGTCTTCCCGCAACGCCTCAACATGGGCGTC ACGCGAGATCTGTCGCTCATCGCCGAAGCGGAACGCCACACGTCGGACGCCACGGCGAGC ATGGGTGCCCATTGGACCTTTGCGCCGACGACGGACCTCCAACGCGACCCGCGCTGGGGC CGATTTTTCGAAGGCATCTCGGAAGACCCGAAGCTGGAGGCGGATGTCAGCCGAGCCCGA GCGCGAGCCCTCGAAGACGACGATCGCCTCACGGCCTGCGTGAAGCACTTCGCGGCGTAC TCGGTGCCGAACAATGGCAATGATCGAGCGCCCGCCAGCACGAGCCTCCGAGACCTCCGC ACGAACATCCTCCCGCCGTACCGAGAGGCGCTGGAATCGGAGCCCGGCACGGTCATGGTC AACTCGGGTAGCATCAACGGCGTGCCCGCCCACGCCTCCCACTGGCTCCTCACGACGCTC CTCCGCGACACGTACGGCTACGAGGGGATGGTCGTGTCGGATTGGGACGATCTCAATCGC ATGATCACGAATCACGACTATGCCCCCGATTTCGAGACGGCGACGGAGATGGCGATCAAT GCGGGCGTCGATATGTATATGATCGGTAACGGTGGGGATGCCCCGGGTCCCGTCCAATTC ATCGACACCGTGGTGTCGCTGGTCGAGGACGGTGCGATCCCGATGGAGCGAATCGACGAG GCGGTCCGCCGCATTCTCGAGCTCAAGGCCGACCTGGGCCTCTTTGAACAACCGACGGTC GACGAATCGCGAATTGAGACGGTCCTGGGTGGTGCGCAGGAGACCGCCGAGACGATGGCG AAAGAGTCGATGGTGCTCCTGAAGAACACCGACGATACGCTCCCGCTCTCGGGCGATGAG TCCGTCCTCCTGACGGGTCCGGGTGTGGACTCGAATGGCAATAATACGCGAGCCCTCATG CAGCATGGTGGTTGGACGCTCGGCTGGCAAGGCGCCAGCGCCGGTGGCCCGTTTCCGCGC CAGAACCTGCTGGAGGCCGAACTCCGAGCGCGAGTCGGCTCCCTCACGCACGTCCCGACG AGCTACGAGAATACCACCTGGTGGGCGGGTGAAGGCGACGGCGAGAACCAGCAGTCGGAT GAAAATGGTAATTTCGACTTCACGGCGGAGCAGCGCTCGCGGGTCGAATCCGCCGGTCCC GAATCGGATGTGGTGGTCGTGGTCCTGGGGGAAGGCACGCACAACGAGGGCTTCGGCGAC CGAGATGAACTCGTGCTCGACGAGTCGCAGCAGGCGCTCCTCGATACCGTGGTGGAATCG ACGGATGACAGCACGCCGATCATCGGGGTCATGCTGGCCGGCTCGCCCCGAGGCTCCCCC GAGACGTTTAGCCAGCTCGATGCCCTCCTCTTTGCGGGTCAACCGGGTAGCGACGGGGGT GTCGCGATTGCGGAGACGCTGGTCGGCGAGTACAACCCCTCGGGTAAGCTCCCGTTCTCG TGGCCGGAGAATGTCGGCACGACCCCGGTCCAGTACACGCGCTACGACCCGACGTCCACG GGTGGTACGGACAACACGGCGATCTACGAATATGGGCACGGCCTGTCGTACACGGATTTC GAGTACGGCTCGGTCAGCGTGACGCAGCCGACCGTCGGCAGCCCGGCCAACCAATCCGAG GTCACCGTGTCGGTGGAGGTGACGAACACGGGTCGCATGGCCGGTGAGCACATTGTCGAG GTCTTCAACACGCAGTCGTATGGGACGGTCCTCCAACCGCGACGACGACTCCTCGGTTAC GAACGCGTCTCGCTGGGTCCCGGGGAACTGACGACCGTGGATGTCACGGTGGATCTCACG GCCCTGGAAGTGGTGCCCGGTGACGTGCTGGGCCTCGGTCCGAAGGTCGTCGAAGCGGGG GAATACGAGCTGACGGTGGGCCAAGACGGCCCGACGGCCACCCTCACCGTCCAGAATACG GCGTCGATCACGGATCCCGATCCGGTGCCGGGTCTGCCGGACTGGTCCGGCTCCACGCCC GGTCGCGGCAACTCGGGCAACAACGGCAACGGTAACAATACGTCGCGAGACCCCACGATG GAGGATGTCGTGGACCTCCTGGATGAGGTCCGCGGTCGCTCGTAG