Difference between revisions of "Team:Michigan/Notebook/September"
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| − | <li>cloning not working probably because the insert is almost as big as backbone | + | <li>cloning not working probably because the insert is almost as big as backbone let’s transform LacZ again</li> |
| − | let’s transform LacZ again</li> | + | <li> transform in vitro LacZ part again</li> |
| − | <li transform in vitro LacZ part again</li> | + | |
<li>plasmid is ready, digest with HindiIII and A</li> | <li>plasmid is ready, digest with HindiIII and A</li> | ||
<li>gel extract plasmid, measure dna concentration</li> | <li>gel extract plasmid, measure dna concentration</li> | ||
Revision as of 03:50, 19 September 2015
September
Work plan:
LacZ into Thrombin plasmid:
- cloning not working probably because the insert is almost as big as backbone let’s transform LacZ again
- transform in vitro LacZ part again
- plasmid is ready, digest with HindiIII and A
- gel extract plasmid, measure dna concentration
LacZ into Gliadin plasmid:
Pfldh plasmid with GFP:
measure fluorescence
Things to order:
- aptamer and trigger for Gliadin
- switch for Gliadin with GFP (check design folder)
- PfLDH protein
- aptamer and trigger for Pfldh
- aptamer and trigger for Kanamycin
