Difference between revisions of "Team:UCLA/Notebook/Protein Cages/12 July 2015"
(Created page with "{{Template:UCLA}} Introduction: Starter culture will be done today. Procedures: 10mL of LB was added to a culture tube. For a final concentration of 100ug/mL of carbenicilli...") |
|||
| Line 5: | Line 5: | ||
Procedures: | Procedures: | ||
| − | 10mL of LB was added to a culture tube. For a final concentration of 100ug/mL of carbenicillin, 10uL of 100mg/mL was added to the 10mL of LB. Using a sterile pipette tip, and pipette, a single colony was streaked and the entire tip was ejected into the culture tube. The tube was then incubated at 37C at 5:50PM. | + | 10mL of LB was added to a culture tube. For a final concentration of 100ug/mL of carbenicillin, 10uL of 100mg/mL was added to the 10mL of LB. Using a sterile pipette tip, and pipette, a single colony was streaked and the entire tip was ejected into the culture tube. The tube was then incubated at 37C at 5:50PM. In addition, another 1L of LB was made and autoclaved for 30 minutes. |
Revision as of 02:01, 16 July 2015
Introduction: Starter culture will be done today.
Procedures:
10mL of LB was added to a culture tube. For a final concentration of 100ug/mL of carbenicillin, 10uL of 100mg/mL was added to the 10mL of LB. Using a sterile pipette tip, and pipette, a single colony was streaked and the entire tip was ejected into the culture tube. The tube was then incubated at 37C at 5:50PM. In addition, another 1L of LB was made and autoclaved for 30 minutes.