Difference between revisions of "Team:UCLA/Notebook/Protein Cages/12 July 2015"

 
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Phillip's notes:
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Introduction:  Starter culture will be done today.
 
Introduction:  Starter culture will be done today.

Latest revision as of 02:09, 16 July 2015

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Phillip's notes:


Introduction: Starter culture will be done today.

Procedures:

10mL of LB was added to a culture tube. For a final concentration of 100ug/mL of carbenicillin, 10uL of 100mg/mL was added to the 10mL of LB. Using a sterile pipette tip, and pipette, a single colony was streaked and the entire tip was ejected into the culture tube. The tube was then incubated at 37C at 5:50PM. In addition, another 1L of LB was made and autoclaved for 30 minutes.