Difference between revisions of "Team:UCLA/Notebook/Spider Silk Genetics/22 July 2015"

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*Ran digest on 1.5% TAE gel. Used 2 uL of 50 bp ladder.
 
*Ran digest on 1.5% TAE gel. Used 2 uL of 50 bp ladder.
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[[File:7 22 2015 UCLA ICA.jpg|none|thumb|500px|'''Fig. 1'''BsaI Digestion of MaSp2 Seq AB. The Expected product size is 102 bp.]]
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*The digestion worked, but it is not as complete as previous digestions have been. Compare to [https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/13_July_2015#BsaI Digestion of MaSp2 Plasmids and Gel Purification previously].

Revision as of 20:29, 26 July 2015

iGEM UCLA




7/22/2015

Transformation Results

  • Colonies are present on all plates from yesterday.

Miniprep

  • Used Zymo Classic kit to prep M2-9(T7) and M2-SeqAB
Concentration (ng/uL) A 260/280
M2-9(T7)-1 590.49 1.86
M2-9(T7)-2 614.07 1.84
M2-9(T7)-3 392.19 1.84
M2-Seq AB 1117.69 1.88

BsaI Digestion for M2-Seq AB

  • Digest 5 ug of M2-SeqAB plasmid in 50 uL reaction with 4 uL of BsaI.
Volume (uL)
BsaI 4
10x Cutsmart 5
DNA 4.47
ddH2O 36.53
  • Ran digest on 1.5% TAE gel. Used 2 uL of 50 bp ladder.
Fig. 1BsaI Digestion of MaSp2 Seq AB. The Expected product size is 102 bp.