Team:UCLA/Notebook/Protein Cages/21 August 2015

iGEM UCLA




Intro: Grew the starter culture overnight for ~16 hours. Refreshed into 300 mL expression culture. Induced with IPTG.

Expression Culture:

OD with 4 mL of starter culture: 0.023

OD with all of starter culture: 0.049

Incubation with all of starter culture began at 1:25 pm.

OD after one hour: 0.073

OD after two hours: 0.112

OD after 3.5 hours: 0.443

OD after 4.5 hours: 1.091

Induced with IPTG to 1mM concentration at ~6:20pm.

OD with IPTG after one hour: 1.370