Team:Czech Republic/Microfluidics
Microfluidics
Contents
Introduction
Microfluidic technology showed different perspective of the fluid and cell handling. The diffusion processes are slow, and the inertial effects are negligible on micro-scale with low Reynolds number [6,55]. It became a valuable tool enabling complex control of the cellular microenvironment.
Soft lithography description [ Fikar2015].
Soft-lithography
Microfluidic channels were formed using PDMS soft-lithography technology, which has proven biocompatibility and can be readily applied in available laminar flow cabinets (photomask and silicon master fabrication is outsourced). Silicon masters were required for the PDMS molding. PDMS molds were bonded to the glass substrates to form encapsulated microfluidic devices using air plasma technology.
Experimental setup
Microfluidic experiments were conducted on a platform already established by the Georgiev lab. The laboratory is equipped with precise microfluidic syringe system (neMESYS Low Pressure Syringe Pumps), and microscopic station enabling fluorescence imaging and live cell microscopy (Olympus IX83).
Appendix
Personnel
Martin Cienciala - Responsible person
Pavel Fikar - Scientific advisor
References
- ↑ Lin, C.-H., Choi, a., & Bennett, R. J. (2011). Defining pheromone-receptor signaling in Candida albicans and related asexual Candida species. Molecular Biology of the Cell, 22(24), 4918–4930. doi:10.1091/mbc.E11-09-0749