Team:UC San Diego/Notebook
TIMELINE
September 24-28
Giant Jamboree!
Computational
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WEEK 14Wet Lab
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WEEK 14Title of section 2
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Section 13
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WEEK 13Wet Lab
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WEEK 13
Section 13
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WEEK 12week ##
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WEEK 12Section 13
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WEEK 11Section 13
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WEEK 11week ##
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WEEK 11Section 13
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WEEK 10Section 13
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WEEK 10week 10
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WEEK 10week 9
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WEEK 9/span>week 9
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WEEK 9week 9
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WEEK 9
Section 13
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WEEK 8week 8
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WEEK 8Section 13
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WEEK 7Section 13
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WEEK 7week 7
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WEEK 7Section 13
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WEEK 6Section 13
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WEEK 6week 6
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WEEK 6Section 13
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WEEK 5Section 13
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WEEK 5week 5
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WEEK 5Section 13
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WEEK 4Section 13
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WEEK 4week 4
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WEEK 4Section 13
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WEEK 3Section 13
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WEEK 3week 3
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WEEK 3Website
More website ideas.
WEEK 2Computational
Read papers on drive
Read about metabolic control analysis
Compiled notes on bioluminescent system
Literature research:understanding bioluminescent mechanism
> aldehyde synthesis
> FMNH2 production
> light production
Familiarized with COBRA module
Primer to Genome-Scale Modeled and COBRA Tutorial (Jahir)
Continued literature search for key mechanisms regarding our auto-induced bioluminescent reaction in a yeast model
>auto-induced therefore no coupling between cells (removed LuxI and LuxR genes)
Wet Lab
Created a preliminary design for the plasmids using ApE.
Improved them over the week by adding tags, removed illegal restriction sites, and changing repetitive sequences.
Design the interlab protocol sequence.
Website
Discussed and designed website prototypes.
Week 1Computational
Developed Primer to MATLAB Programming
> lecture material from MIT course and UCSD course
> reading material from UCSD course
> study and practice material MIT course and UCSD course
Wet Lab
+ Found genes coding for fatty acid reductase complex that have been validated in an in vitro synthesis paper.
+ Planned how to assemble our plasmids and determined nucleotide sequences for lux A-E of Photobacterium Phosphoreum.
+ Compared the amino acid sequences to those of other organisms on BLAST to check for significant discrepancies in our sequence.