Team:NRP-UEA-Norwich/Parts

Parts

BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents	Backbone	Description	Function	Part number
GlgX	pSB1C3	Debranching enzyme (E.coli)	Coding sequence only	BBa_K1618025
GlgB	pSB1C3	Branching Enzyme (E.coli)	Coding sequence only	BBa_K1618000
MAO	pSB1C3	Acyl-transferase Candidate 1	Coding sequence only	BBa_K1618001
Rv3030	pSB1C3	Acylation candidate 2	Coding sequence only	BBa_K1618002
Rv3034c	pSB1C3	Acylation candidate 3	Coding sequence only	BBa_K1618003
Rv3037c	pSB1C3	Acylation candidate 4	Coding sequence only	BBa_K1618004

COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents	Backbone	Description	Function	Part number
P-Lac(R0010)_RBS(B0034)_GlgX(K1618026)	pSB1C3	Debranching enzyme expression construct	Removes glycogen branches	BBa_K1618026
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)	pSB1C3	Branching enzyme expression construct	Add glycogen branches	BBa_K1618022
P-Lac(R0010)_RBS(B0034)_MAO(K1618001)	pSB1C3	Acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618005
P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002)	pSB1C3	Acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618007
P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003)	pSB1C3	Acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618008
P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004)	pSB1C3	Acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618009
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_MAO(K1618001)	pSB1C3	Branching enzyme + acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618023
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002)	pSB1C3	Branching enzyme + acyl-transferase expression construct	)Transfers acyl groups to glycogen	BBa_K1618023
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003)	pSB1C3	Branching enzyme + acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618024
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004)	pSB1C3	BBranching enzyme + acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618027

COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (CODING SEQUNCES WERE FROM Coprococcus sp. L2-50 (DSM 16842)

Part Contents	Backbone	Description	Function	Part number
P-Lac(R0010)_RBS(B0034)_Buk	pSB1C3	Butyrate kinase expression contruct	Catalyzes the conversionbutyrate to/from butyryl	BBa_K1618012
P-Lac(R0010)_RBS(B0034)_PTB	pSB1C3	Phosphotransbutrylase expression construct	Catalyzes the conversion butyryl to/from butyryl CoA	BBa_K1618013
P-Lac(R0010)_RBS(B0034)_Buk_PTB	pSB1C3	utyrate to butyryl CoA pathway	Catalyzes the conversion butyryl to/from butyryl CoA	BBa_K1618020
P-Lac(R0010)_RBS(B0034)_THL	pSB1C3	Thiolase expression construct	Catalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA	BBa_K1618014
P-Lac(R0010)_RBS(B0034)_CRO	pSB1C3	Crotonase expression construct	Catalyzes conversion of3-hydroxybutyryl-CoA to Crotonyl-CoA	BBa_K1618015
P-Lac(R0010)_RBS(B0034)_BHBD	pSB1C3	β-hydroxybutyryl-CoA dehydrogenase expression	Catalyzes conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA	BBa_K1618016
P-Lac(R0010)_RBS(B0034)_BCD	pSB1C3	Butyryl-CoA dehydrogenase expression construct	Catalyzes conversion of Crotonyl-CoA to Butyryl-CoA	BBa_K1618017
P-Lac(R0010)_RBS(B0034)_EFTb	pSB1C3	Electron transfer protein β expression construct	Catalyzes conversion of acetate to butyrate	BBa_K1618018
P-Lac(R0010)_RBS(B0034)_EFTa	pSB1C3	Electron transfer protein α expression construct	Catalyzes conversion of acetate to butyrate	BBa_K1618019
P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD	pSB1C3	Acetyl-CoA to 3-crotonyl-CoA pathway	Catalyzes conversion of Acetyl-CoA to 3-crotonyl-CoA	--------
P-Lac(R0010)_RBS(B0034)_EFTa	pSB1C3	Crotonyl-CoA to Butyryl-coA pathway	Catalyzes conversion of Crotonyl-CoA to Butyryl-coA	-----------
P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa	pSB1C3	Acetyl-coA to Butryl-CoA pathway	Catalyzes conversion of Acetyl-coA to Butryl-CoA	BBa_K1618021
P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTa	pSB1C3	Acetyl coA to butyrate complete biosynthetic pathway	Catalyzes conversion of Acetyl coA to butyrate	----------

Plac-GlgXpSB1C3Debranching enzyme with IPTG inducible PromoterRemoves E. coli glycogen branchesBBa_K1618025 GlgXpSB1C3Debranching enzyme  BBa_K1618026 Plac-GlgBpSB1C3Branching enzyme with IPTG inducible PromoterAdd E. coli glycogen branchesBBa_K1618022 GlgBpSB1C3Branching Enzyme  BBa_K1618000 Plac-MAOpSB1C3Acylation candidate 1 with IPTG inducible PromoterAcylates glycogen? (1)BBa_K1618005 MAOpSB1C3Acylation Candidate 1  BBa_K1618001 Plac-Rv3030pSB1C3Acylation candidate 2 with IPTG inducible PromoterAcylates glycogen? (2)BBa_K1618007 Rv3030pSB1C3Acylation candidate 2 BBa_K1618002 Plac-Rv3034cpSB1C3Acylation candidate 3 with IPTG inducible PromoterAcylates glycogen? (3) BBa_K1618008 Rv3034cpSB1C3Acylation candidate 3  BBa_K1618003 Plac-Rv3037cpSB1C3Acylation candidate 4 with IPTG inducible PromoterAcylates glycogen? (4) BBa_K1618009 Rv3037cpSB1C3Acylation candidate 4  BBa_K1618004 Plac-GlgB-Rv3030pSB1C3Branching enzyme + Acylation candidate 2 with IPTG inducible PromoterMakes acetylated branched glycogen? (2)BBa_K1618023 Plac-GlgB-Rv3034cpSB1C3Branching enzyme + Acylation candidate 3 with IPTG inducible PromoterMakes acetylated branched glycogen? (3)BBa_K1618024 Plac-GlgB-Rv3037cpSB1C3Branching enzyme + Acylation candidate 4 with IPTG inducible PromoterMakes acetylated branched glycogen? (4)BBa_K1618027 Plac-BukpSB1C3Butyrate kinase with IPTG inducible PromoterReversible reaction that makes butyrate from butyryl phosphateBBa_K1618012 Plac-PTBpSB1C3Phosphotransbutrylase with IPTG inducible PromoterReversible reaction that makes butyryl-phosphate from butyryl-CoABBa_K1618013 Plac-THLpSB1C3Thiolase with IPTG inducible PromoterMakes Acetoacetyl-CoA from Acetyl-CoABBa_K1618014 Plac-CROpSB1C3Crotonase with IPTG inducible PromoterMakes Crotonyl-CoA from 3-hydroxybutyryl-CoABBa_K1618015 Plac-BHBDpSB1C3β-hydroxybutyryl-CoA dehydrogenase with IPTG inducible PromoterMakes 3-hydroxybutyryl-CoA from Acetoacetyl-CoA BBa_K1618016 Plac-BCDpSB1C3Butyryl-CoA dehydrogenase with IPTG inducible PromoterMakes Butyryl-CoA from Crotonyl-CoABBa_K1618017 Plac-EFTbpSB1C3Electron transfer protein β with IPTG inducible PromoterMakes butyrate from acetate part 5BBa_K1618018 Plac-EFTapSB1C3Electron transfer protein α with IPTG inducible PromoterMakes butyrate from acetate part 6BBa_K1618019 Plac-Buk-PTBpSB1C3Butyrate to butyryl CoA pathway with IPTG inducible Promoter Reversible reaction that makes Butyrate from Butyryl-CoABBa_K1618020 Plac-THL-CRO-BHBD-BCD-EFTb-EFTapSB1C3Acetyl-CoA to Butyrate pathway with IPTG inducible Promoter Makes butyrate from Acetyl-CoA fullBBa_K1618021 35S-MAO?Acylation candidate 1 with 35S PromoterAcetylates starch? (1)BBa_K1618033 35S-Rv3030?Acylation candidate 2 with 35S PromoterAcetylates starch? (2)BBa_K1618034 35S-Rv3034c?Acylation candidate 3 with 35S PromoterAcetylates starch? (3)BBa_K1618035 35S-Rv3037c?Acylation candidate 4 with 35S PromoterAcetylates starch? (4)BBa_K1618036 35S-GFP-MAO?Acylation candidate 1 with 35S Promoter + GFPAcetylates starch? (1) + GFP tagBBa_K1618029 35S-GFP-Rv3030?Acylation candidate 2 with 35S Promoter + GFPAcetylates starch? (2) + GFP tagBBa_K1618030 35S-GFP-Rv3034c?Acylation candidate 3 with 35S Promoter + GFPAcetylates starch? (3) + GFP tagBBa_K1618031 35S-GFP-Rv3037c?Acylation candidate 4 with 35S Promoter + GFPAcetylates starch? (4) + GFP tagBBa_K1618032 Chloroplast Transit Peptide? Chloroplast Transit PeptideBBa_K1618028 35s Terminator? TerminatorBBa_K1618037

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