Team:SZU China/Notebook

Notebook

Combine Biological Unnatural Amino Acid（UAA）Orthogonal
genectical system with logic "AND" gate gene circuit
to detect bladder cancer cells precisely

• 4/10/13 April 2nd - April 23rd

  To discuss the decision of team project: CANCER SNIPER BLADDER

• 4/11/13 April 28th - June 20th

  Finished the project system in shuttle vector

• 4/13/13 June 26th

  Transfected the two plasmid system in 5637 and T24 bladder cancer cells,and got a good result in Rluc(with amber mutation). Moreover,we got the control experiments data in human bladder fiber epithelial normal cells(HFC).

• 4/15/13 June 30th

  Improved the original BioBrick TERT (BBa_K1722001).

• 4/16/13 July 10th - July 25th

  In order to increase the expression of the system,we remoulded the system in three palsmid.

• 4/16/13 July 12th

  We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.

• 4/16/13 July 18th

  Began to distribute questionnaires Onlinehttp://www.sojump.com/jq/5569949.aspx

• 4/16/13 July 28th

  Transfected the three plasmid system in the T24 and 5637 bladder cancer cells,but its result was not so good.Therefore,we continued to use the two plasmid system.

• 4/16/13 July 30th

  In order to increase the expression of the system,we remoulded the system in three palsmid.