Team:Genspace/Notebook
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SuperFUNd Gowanus!
The Gowanus Canal is a heavily polluted waterway that runs through Brooklyn NY. A designated superfund site, it is slated for cleanup but nearby residents are concerned about the results. Our team is developing a biosensor for waste pollution, giving the community real time access to data on the health of the canal. Additionally we have mined the canal for extremophiles with interesting properties.
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Notebook
Week 1 (June 2 - June 8)
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Wet Lab Overview
We have finally done stuff! Student team members continued their initial safety training, as well as learning how to do basic techniques (e.g. LB preps, 60% Glycerol stock, Colony selections and Plasmid preps). Meanwhile, our senior team members came up with a more detailed plan than just "biosensor". By modifying Lsr Operons, we hope to use Quorum sensing to detect the presence of E.coli bacteria other than those that we use. Training continued today (07/02/15) by introducing students to plasma preps. The "Quorum" group looked up two parts we need for the sensing project(LsrR and such), and decided to teach the students how to take DNA out of the registry. The first day of Wet Lab training began on the 7th! We started working on amplification of metallothionein genes - so far nixA and GST-PMT appear to be working.
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Dry Lab Overview
Today was entirely dedicated to researching, spiting the team in two to make the most efficient use of our time. The adults and undergraduates focused on quorum sensing. The high school students looked through different promoters that activated in the presence of molecules common to human sewage.
Inspiration
You can see what others teams have done to organize their notes: